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Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy

Bile acids (BAs), endogenous acidic steroids synthetized from cholesterol in the liver, play a key role in the gut–liver axis physiopathology, including in hepatotoxicity, intestinal inflammatory processes, and cholesterol homeostasis. Faecal Oxo-BAs, relatively stable intermediates of oxidation/epi...

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Autores principales: Comito, Rossana, Porru, Emanuele, Interino, Nicolò, Conti, Matteo, Terragni, Rossella, Gotti, Roberto, Candela, Marco, Simoni, Patrizia, Roda, Aldo, Fiori, Jessica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535270/
https://www.ncbi.nlm.nih.gov/pubmed/37755260
http://dx.doi.org/10.3390/metabo13090980
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author Comito, Rossana
Porru, Emanuele
Interino, Nicolò
Conti, Matteo
Terragni, Rossella
Gotti, Roberto
Candela, Marco
Simoni, Patrizia
Roda, Aldo
Fiori, Jessica
author_facet Comito, Rossana
Porru, Emanuele
Interino, Nicolò
Conti, Matteo
Terragni, Rossella
Gotti, Roberto
Candela, Marco
Simoni, Patrizia
Roda, Aldo
Fiori, Jessica
author_sort Comito, Rossana
collection PubMed
description Bile acids (BAs), endogenous acidic steroids synthetized from cholesterol in the liver, play a key role in the gut–liver axis physiopathology, including in hepatotoxicity, intestinal inflammatory processes, and cholesterol homeostasis. Faecal Oxo-BAs, relatively stable intermediates of oxidation/epimerization reactions of the BA hydroxyls, could be relevant to investigating the crosstalk in the liver–gut axis and the relationship between diseases and alterations in microbiota composition. A paucity of information currently exists on faecal BA profiles in dogs with and without chronic inflammatory enteropathy (CIE). Comprehensive assessment of 31 molecules among faecal BAs and related microbiota metabolites was conducted with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Odds ratios (ORs) for associations of BAs with CIE were estimated using logistic regression. Principal component analysis was performed to find differences between the control and pathological dogs. Higher levels of primary BAs and muricholic acids, and lower levels of secondary BAs were found in pathological dogs. Higher concentrations in faecal oxo-metabolites were associated with the absence of CIE (OR < 1). This study shows a marked difference in faecal BA profiles between dogs with and without CIE. Further research will be needed to better understand the role of oxo-BAs and muricholic acids in CIE dogs.
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spelling pubmed-105352702023-09-29 Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy Comito, Rossana Porru, Emanuele Interino, Nicolò Conti, Matteo Terragni, Rossella Gotti, Roberto Candela, Marco Simoni, Patrizia Roda, Aldo Fiori, Jessica Metabolites Article Bile acids (BAs), endogenous acidic steroids synthetized from cholesterol in the liver, play a key role in the gut–liver axis physiopathology, including in hepatotoxicity, intestinal inflammatory processes, and cholesterol homeostasis. Faecal Oxo-BAs, relatively stable intermediates of oxidation/epimerization reactions of the BA hydroxyls, could be relevant to investigating the crosstalk in the liver–gut axis and the relationship between diseases and alterations in microbiota composition. A paucity of information currently exists on faecal BA profiles in dogs with and without chronic inflammatory enteropathy (CIE). Comprehensive assessment of 31 molecules among faecal BAs and related microbiota metabolites was conducted with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Odds ratios (ORs) for associations of BAs with CIE were estimated using logistic regression. Principal component analysis was performed to find differences between the control and pathological dogs. Higher levels of primary BAs and muricholic acids, and lower levels of secondary BAs were found in pathological dogs. Higher concentrations in faecal oxo-metabolites were associated with the absence of CIE (OR < 1). This study shows a marked difference in faecal BA profiles between dogs with and without CIE. Further research will be needed to better understand the role of oxo-BAs and muricholic acids in CIE dogs. MDPI 2023-08-30 /pmc/articles/PMC10535270/ /pubmed/37755260 http://dx.doi.org/10.3390/metabo13090980 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Comito, Rossana
Porru, Emanuele
Interino, Nicolò
Conti, Matteo
Terragni, Rossella
Gotti, Roberto
Candela, Marco
Simoni, Patrizia
Roda, Aldo
Fiori, Jessica
Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy
title Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy
title_full Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy
title_fullStr Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy
title_full_unstemmed Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy
title_short Metabolic Bile Acid Profile Impairments in Dogs Affected by Chronic Inflammatory Enteropathy
title_sort metabolic bile acid profile impairments in dogs affected by chronic inflammatory enteropathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535270/
https://www.ncbi.nlm.nih.gov/pubmed/37755260
http://dx.doi.org/10.3390/metabo13090980
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