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Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study

As plant-based diets become more popular, there is an interest in developing innovations to improve the bioaccessibility of plant protein. In this study, seven probiotic strains (Bifidobacterium animalis subsp. lactis B420, B. lactis Bl-04, Lactobacillus acidophilus NCFM, Lacticaseibacillus rhamnosu...

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Autores principales: Marttinen, Maija, Anjum, Mehreen, Saarinen, Markku T., Ahonen, Ilmari, Lehtinen, Markus J., Nurminen, Päivi, Laitila, Arja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535511/
https://www.ncbi.nlm.nih.gov/pubmed/37764689
http://dx.doi.org/10.3390/nu15183905
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author Marttinen, Maija
Anjum, Mehreen
Saarinen, Markku T.
Ahonen, Ilmari
Lehtinen, Markus J.
Nurminen, Päivi
Laitila, Arja
author_facet Marttinen, Maija
Anjum, Mehreen
Saarinen, Markku T.
Ahonen, Ilmari
Lehtinen, Markus J.
Nurminen, Päivi
Laitila, Arja
author_sort Marttinen, Maija
collection PubMed
description As plant-based diets become more popular, there is an interest in developing innovations to improve the bioaccessibility of plant protein. In this study, seven probiotic strains (Bifidobacterium animalis subsp. lactis B420, B. lactis Bl-04, Lactobacillus acidophilus NCFM, Lacticaseibacillus rhamnosus HN001, Lacticaseibacillus paracasei subsp. paracasei Lpc-37, Lactiplantibacillus plantarum Lp-115, and Lactococcus lactis subsp. lactis Ll-23) were evaluated for their capacity to hydrolyze soy and pea protein ingredients in an in vitro digestion model of the upper gastrointestinal tract (UGIT). Compared to the control digestion of protein without a probiotic, all the studied strains were able to increase the digestion of soy or pea protein, as evidenced by an increase in free α-amino nitrogen (FAN) and/or free amino acid concentration. The increase in FAN varied between 13 and 33% depending on the protein substrate and probiotic strain. The survival of probiotic bacteria after exposure to digestive fluids was strain-dependent and may have affected the strain’s capacity to function and aid in protein digestion in the gastrointestinal environment. Overall, our results from the standardized in vitro digestion model provide an approach to explore probiotics for improved plant protein digestion and bioaccessibility of amino acids; however, human clinical research is needed to evaluate the efficacy of probiotics on amino acid absorption and bioavailability in vivo.
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spelling pubmed-105355112023-09-29 Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study Marttinen, Maija Anjum, Mehreen Saarinen, Markku T. Ahonen, Ilmari Lehtinen, Markus J. Nurminen, Päivi Laitila, Arja Nutrients Article As plant-based diets become more popular, there is an interest in developing innovations to improve the bioaccessibility of plant protein. In this study, seven probiotic strains (Bifidobacterium animalis subsp. lactis B420, B. lactis Bl-04, Lactobacillus acidophilus NCFM, Lacticaseibacillus rhamnosus HN001, Lacticaseibacillus paracasei subsp. paracasei Lpc-37, Lactiplantibacillus plantarum Lp-115, and Lactococcus lactis subsp. lactis Ll-23) were evaluated for their capacity to hydrolyze soy and pea protein ingredients in an in vitro digestion model of the upper gastrointestinal tract (UGIT). Compared to the control digestion of protein without a probiotic, all the studied strains were able to increase the digestion of soy or pea protein, as evidenced by an increase in free α-amino nitrogen (FAN) and/or free amino acid concentration. The increase in FAN varied between 13 and 33% depending on the protein substrate and probiotic strain. The survival of probiotic bacteria after exposure to digestive fluids was strain-dependent and may have affected the strain’s capacity to function and aid in protein digestion in the gastrointestinal environment. Overall, our results from the standardized in vitro digestion model provide an approach to explore probiotics for improved plant protein digestion and bioaccessibility of amino acids; however, human clinical research is needed to evaluate the efficacy of probiotics on amino acid absorption and bioavailability in vivo. MDPI 2023-09-07 /pmc/articles/PMC10535511/ /pubmed/37764689 http://dx.doi.org/10.3390/nu15183905 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marttinen, Maija
Anjum, Mehreen
Saarinen, Markku T.
Ahonen, Ilmari
Lehtinen, Markus J.
Nurminen, Päivi
Laitila, Arja
Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study
title Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study
title_full Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study
title_fullStr Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study
title_full_unstemmed Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study
title_short Enhancing Bioaccessibility of Plant Protein Using Probiotics: An In Vitro Study
title_sort enhancing bioaccessibility of plant protein using probiotics: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535511/
https://www.ncbi.nlm.nih.gov/pubmed/37764689
http://dx.doi.org/10.3390/nu15183905
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