Cargando…

Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics

The ability to simultaneously heat and image samples using transmitted light is crucial for several biological applications. However, existing techniques such as heated stage microscopes, thermal cyclers equipped with imaging capabilities, or non-contact heating systems are often bulky, expensive, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Laman, Alex, Das, Debayan, Priye, Aashish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535971/
https://www.ncbi.nlm.nih.gov/pubmed/37765775
http://dx.doi.org/10.3390/s23187718
_version_ 1785112756025819136
author Laman, Alex
Das, Debayan
Priye, Aashish
author_facet Laman, Alex
Das, Debayan
Priye, Aashish
author_sort Laman, Alex
collection PubMed
description The ability to simultaneously heat and image samples using transmitted light is crucial for several biological applications. However, existing techniques such as heated stage microscopes, thermal cyclers equipped with imaging capabilities, or non-contact heating systems are often bulky, expensive, and complex. This work presents the development and characterization of a Miniaturized Optically-clear Thermal Enclosure (MOTE) system—an open-source, inexpensive, and low-powered modular system—capable of convectively heating samples while simultaneously imaging them with transmitted light. We develop and validate a computational fluid dynamics (CFD) model to design and optimize the heating chamber. The model simulates velocity and temperature profiles within the heating chamber for various chamber materials and sizes. The computational model yielded an optimal chamber dimension capable of achieving a stable temperature ranging from ambient to 95 °C with a spatial discrepancy of less than 1.5 °C, utilizing less than 8.5 W of power. The dual-functionality of the MOTE system, enabling synchronous heating and transmitted light imaging, was demonstrated through the successful execution of paper-based LAMP reactions to detect λ DNA samples in real-time down to 10 copies/µL of the target concentration. The MOTE system offers a promising and flexible platform for various applications, from molecular diagnostics to biochemical analyses, cell biology, genomics, and education.
format Online
Article
Text
id pubmed-10535971
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105359712023-09-29 Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics Laman, Alex Das, Debayan Priye, Aashish Sensors (Basel) Article The ability to simultaneously heat and image samples using transmitted light is crucial for several biological applications. However, existing techniques such as heated stage microscopes, thermal cyclers equipped with imaging capabilities, or non-contact heating systems are often bulky, expensive, and complex. This work presents the development and characterization of a Miniaturized Optically-clear Thermal Enclosure (MOTE) system—an open-source, inexpensive, and low-powered modular system—capable of convectively heating samples while simultaneously imaging them with transmitted light. We develop and validate a computational fluid dynamics (CFD) model to design and optimize the heating chamber. The model simulates velocity and temperature profiles within the heating chamber for various chamber materials and sizes. The computational model yielded an optimal chamber dimension capable of achieving a stable temperature ranging from ambient to 95 °C with a spatial discrepancy of less than 1.5 °C, utilizing less than 8.5 W of power. The dual-functionality of the MOTE system, enabling synchronous heating and transmitted light imaging, was demonstrated through the successful execution of paper-based LAMP reactions to detect λ DNA samples in real-time down to 10 copies/µL of the target concentration. The MOTE system offers a promising and flexible platform for various applications, from molecular diagnostics to biochemical analyses, cell biology, genomics, and education. MDPI 2023-09-07 /pmc/articles/PMC10535971/ /pubmed/37765775 http://dx.doi.org/10.3390/s23187718 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Laman, Alex
Das, Debayan
Priye, Aashish
Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
title Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
title_full Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
title_fullStr Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
title_full_unstemmed Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
title_short Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
title_sort miniaturized non-contact heating and transmitted light imaging using an inexpensive and modular 3d-printed platform for molecular diagnostics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535971/
https://www.ncbi.nlm.nih.gov/pubmed/37765775
http://dx.doi.org/10.3390/s23187718
work_keys_str_mv AT lamanalex miniaturizednoncontactheatingandtransmittedlightimagingusinganinexpensiveandmodular3dprintedplatformformoleculardiagnostics
AT dasdebayan miniaturizednoncontactheatingandtransmittedlightimagingusinganinexpensiveandmodular3dprintedplatformformoleculardiagnostics
AT priyeaashish miniaturizednoncontactheatingandtransmittedlightimagingusinganinexpensiveandmodular3dprintedplatformformoleculardiagnostics