Cargando…

The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth

The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Miwa, Yuka, Awanthi, Mahanama Geegana Gamage, Soga, Kouichi, Tanaka, Atsuko, Ito, Michihiro, Numata, Yuichiro, Sato, Yoichi, Konishi, Teruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536011/
https://www.ncbi.nlm.nih.gov/pubmed/37765437
http://dx.doi.org/10.3390/plants12183274
_version_ 1785112765410574336
author Miwa, Yuka
Awanthi, Mahanama Geegana Gamage
Soga, Kouichi
Tanaka, Atsuko
Ito, Michihiro
Numata, Yuichiro
Sato, Yoichi
Konishi, Teruko
author_facet Miwa, Yuka
Awanthi, Mahanama Geegana Gamage
Soga, Kouichi
Tanaka, Atsuko
Ito, Michihiro
Numata, Yuichiro
Sato, Yoichi
Konishi, Teruko
author_sort Miwa, Yuka
collection PubMed
description The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW, and HC-I contained mainly fucoidan composed of Fucose, Glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.6~0.7 and 1.0:0.3:0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslinking with cellulose and strengthening the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and the reduction in water content during the growth process.
format Online
Article
Text
id pubmed-10536011
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105360112023-09-29 The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth Miwa, Yuka Awanthi, Mahanama Geegana Gamage Soga, Kouichi Tanaka, Atsuko Ito, Michihiro Numata, Yuichiro Sato, Yoichi Konishi, Teruko Plants (Basel) Article The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW, and HC-I contained mainly fucoidan composed of Fucose, Glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.6~0.7 and 1.0:0.3:0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslinking with cellulose and strengthening the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and the reduction in water content during the growth process. MDPI 2023-09-15 /pmc/articles/PMC10536011/ /pubmed/37765437 http://dx.doi.org/10.3390/plants12183274 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miwa, Yuka
Awanthi, Mahanama Geegana Gamage
Soga, Kouichi
Tanaka, Atsuko
Ito, Michihiro
Numata, Yuichiro
Sato, Yoichi
Konishi, Teruko
The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
title The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
title_full The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
title_fullStr The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
title_full_unstemmed The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
title_short The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
title_sort cell wall characterization of brown alga cladosiphon okamuranus during growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536011/
https://www.ncbi.nlm.nih.gov/pubmed/37765437
http://dx.doi.org/10.3390/plants12183274
work_keys_str_mv AT miwayuka thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT awanthimahanamageeganagamage thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT sogakouichi thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT tanakaatsuko thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT itomichihiro thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT numatayuichiro thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT satoyoichi thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT konishiteruko thecellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT miwayuka cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT awanthimahanamageeganagamage cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT sogakouichi cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT tanakaatsuko cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT itomichihiro cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT numatayuichiro cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT satoyoichi cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth
AT konishiteruko cellwallcharacterizationofbrownalgacladosiphonokamuranusduringgrowth