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The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth
The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variatio...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536011/ https://www.ncbi.nlm.nih.gov/pubmed/37765437 http://dx.doi.org/10.3390/plants12183274 |
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author | Miwa, Yuka Awanthi, Mahanama Geegana Gamage Soga, Kouichi Tanaka, Atsuko Ito, Michihiro Numata, Yuichiro Sato, Yoichi Konishi, Teruko |
author_facet | Miwa, Yuka Awanthi, Mahanama Geegana Gamage Soga, Kouichi Tanaka, Atsuko Ito, Michihiro Numata, Yuichiro Sato, Yoichi Konishi, Teruko |
author_sort | Miwa, Yuka |
collection | PubMed |
description | The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW, and HC-I contained mainly fucoidan composed of Fucose, Glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.6~0.7 and 1.0:0.3:0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslinking with cellulose and strengthening the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and the reduction in water content during the growth process. |
format | Online Article Text |
id | pubmed-10536011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105360112023-09-29 The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth Miwa, Yuka Awanthi, Mahanama Geegana Gamage Soga, Kouichi Tanaka, Atsuko Ito, Michihiro Numata, Yuichiro Sato, Yoichi Konishi, Teruko Plants (Basel) Article The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW, and HC-I contained mainly fucoidan composed of Fucose, Glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.6~0.7 and 1.0:0.3:0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslinking with cellulose and strengthening the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and the reduction in water content during the growth process. MDPI 2023-09-15 /pmc/articles/PMC10536011/ /pubmed/37765437 http://dx.doi.org/10.3390/plants12183274 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Miwa, Yuka Awanthi, Mahanama Geegana Gamage Soga, Kouichi Tanaka, Atsuko Ito, Michihiro Numata, Yuichiro Sato, Yoichi Konishi, Teruko The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth |
title | The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth |
title_full | The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth |
title_fullStr | The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth |
title_full_unstemmed | The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth |
title_short | The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth |
title_sort | cell wall characterization of brown alga cladosiphon okamuranus during growth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536011/ https://www.ncbi.nlm.nih.gov/pubmed/37765437 http://dx.doi.org/10.3390/plants12183274 |
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