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Optimization and Characterization of a Novel Antioxidant Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic Diabetic Wounds: A Preclinical Study
[Image: see text] Nonhealed wounds are one of the most dangerous side effects of type-2 diabetes, which is linked to a high frequency of bacterial infections around the globe that eventually results in amputation of limbs. The present investigation aimed to explore the drug-loaded (naringenin) hydro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536028/ https://www.ncbi.nlm.nih.gov/pubmed/37779948 http://dx.doi.org/10.1021/acsomega.3c04441 |
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author | Raina, Neha Haque, Shafiul Tuli, Hardeep Singh Jain, Atul Slama, Petr Gupta, Madhu |
author_facet | Raina, Neha Haque, Shafiul Tuli, Hardeep Singh Jain, Atul Slama, Petr Gupta, Madhu |
author_sort | Raina, Neha |
collection | PubMed |
description | [Image: see text] Nonhealed wounds are one of the most dangerous side effects of type-2 diabetes, which is linked to a high frequency of bacterial infections around the globe that eventually results in amputation of limbs. The present investigation aimed to explore the drug-loaded (naringenin) hydrogel system for chronic wound healing. The hydrogel membranes comprising Na-alginate with F-127 and poly(vinyl alcohol) were developed to treat chronic wounds using the quality-by-design (QbD) approach. The optimized formulation was tested for various parameters, such as swelling, gel fraction, water vapor transition rate (WVTR), etc. In vitro evaluation indicated that a drug-loaded hydrogel displayed better tissue adhesiveness and can release drugs for a prolonged duration of 12 h. Scratch assay performed on L929 cell lines demonstrated good cell migration. The diabetic wound healing potential of the hydrogel membrane was assessed in streptozotocin-induced male Wistar rats (50 mg/kg). Higher rates of wound closure, re-epithelialization, and accumulation of collagen were seen in in vivo experiments. Histopathologic investigation correspondingly implied that the drug-loaded hydrogel could enhance dermal wound repair. The improved antimicrobial and antioxidant properties with expedited healing indicated that the drug-loaded hydrogel is a perfect dressing for chronic wounds. |
format | Online Article Text |
id | pubmed-10536028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105360282023-09-29 Optimization and Characterization of a Novel Antioxidant Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic Diabetic Wounds: A Preclinical Study Raina, Neha Haque, Shafiul Tuli, Hardeep Singh Jain, Atul Slama, Petr Gupta, Madhu ACS Omega [Image: see text] Nonhealed wounds are one of the most dangerous side effects of type-2 diabetes, which is linked to a high frequency of bacterial infections around the globe that eventually results in amputation of limbs. The present investigation aimed to explore the drug-loaded (naringenin) hydrogel system for chronic wound healing. The hydrogel membranes comprising Na-alginate with F-127 and poly(vinyl alcohol) were developed to treat chronic wounds using the quality-by-design (QbD) approach. The optimized formulation was tested for various parameters, such as swelling, gel fraction, water vapor transition rate (WVTR), etc. In vitro evaluation indicated that a drug-loaded hydrogel displayed better tissue adhesiveness and can release drugs for a prolonged duration of 12 h. Scratch assay performed on L929 cell lines demonstrated good cell migration. The diabetic wound healing potential of the hydrogel membrane was assessed in streptozotocin-induced male Wistar rats (50 mg/kg). Higher rates of wound closure, re-epithelialization, and accumulation of collagen were seen in in vivo experiments. Histopathologic investigation correspondingly implied that the drug-loaded hydrogel could enhance dermal wound repair. The improved antimicrobial and antioxidant properties with expedited healing indicated that the drug-loaded hydrogel is a perfect dressing for chronic wounds. American Chemical Society 2023-09-14 /pmc/articles/PMC10536028/ /pubmed/37779948 http://dx.doi.org/10.1021/acsomega.3c04441 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Raina, Neha Haque, Shafiul Tuli, Hardeep Singh Jain, Atul Slama, Petr Gupta, Madhu Optimization and Characterization of a Novel Antioxidant Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic Diabetic Wounds: A Preclinical Study |
title | Optimization and
Characterization of a Novel Antioxidant
Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic
Diabetic Wounds: A Preclinical Study |
title_full | Optimization and
Characterization of a Novel Antioxidant
Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic
Diabetic Wounds: A Preclinical Study |
title_fullStr | Optimization and
Characterization of a Novel Antioxidant
Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic
Diabetic Wounds: A Preclinical Study |
title_full_unstemmed | Optimization and
Characterization of a Novel Antioxidant
Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic
Diabetic Wounds: A Preclinical Study |
title_short | Optimization and
Characterization of a Novel Antioxidant
Naringenin-Loaded Hydrogel for Encouraging Re-Epithelization in Chronic
Diabetic Wounds: A Preclinical Study |
title_sort | optimization and
characterization of a novel antioxidant
naringenin-loaded hydrogel for encouraging re-epithelization in chronic
diabetic wounds: a preclinical study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536028/ https://www.ncbi.nlm.nih.gov/pubmed/37779948 http://dx.doi.org/10.1021/acsomega.3c04441 |
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