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Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat
Tartary buckwheat (Fagopyrum tataricum Gaertn.) is an important pseudocereal crop with excellent edible, nutritional and medicinal values. However, the yield of Tartary buckwheat (TB) is very low due to old-fashioned cultivation techniques, particularly unreasonable application of nitrogen fertilize...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536138/ https://www.ncbi.nlm.nih.gov/pubmed/37779724 http://dx.doi.org/10.3389/fmicb.2023.1240029 |
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author | Qiu, Qingcheng Xiang, Dabing Li, Qiang Wang, Hanlin Wan, Yan Wu, Qi Ye, Xueling Jiang, Liangzhen Fan, Yu Liu, Bingliang Liu, Yanxia Li, Han Liu, Changying |
author_facet | Qiu, Qingcheng Xiang, Dabing Li, Qiang Wang, Hanlin Wan, Yan Wu, Qi Ye, Xueling Jiang, Liangzhen Fan, Yu Liu, Bingliang Liu, Yanxia Li, Han Liu, Changying |
author_sort | Qiu, Qingcheng |
collection | PubMed |
description | Tartary buckwheat (Fagopyrum tataricum Gaertn.) is an important pseudocereal crop with excellent edible, nutritional and medicinal values. However, the yield of Tartary buckwheat (TB) is very low due to old-fashioned cultivation techniques, particularly unreasonable application of nitrogen fertilizer. To improve the understanding on the theories of nitrogen use in TB, the effects of nitrogen application on growth, as well as chemical properties and microbial community of rhizosphere soil were investigated in this study. Nitrogen application could promote the plant height, stem diameter, nitrogen accumulation and yield of TB. The relative abundance and diversity of bacteria and fungi in the rhizosphere soil of TB were improved by nitrogen fertilizer. Nitrogen application increased the abundance of beneficial bacteria such as Lysobacter and Sphingomonas in rhizosphere soil, and decreased the abundance of pathogenic fungi such as Fusarium and Plectosphaerella. The results indicated that nitrogen application changed the distribution of microbial communities in TB rhizosphere soil. Furthermore, the specific enriched or depleted microorganisms in the rhizosphere soil of four TB varieties were analyzed at OTU level. 87 specific nitrogen-responsive genes with sequence variation were identified in four varieties by integrating genomic re-sequencing and transcriptome analysis, and these genes may involve in the recruitment of specific rhizosphere microorganisms in different TB varieties. This study provided new insights into the effects of nitrogen application on TB growth and rhizosphere microbial community, and improved the understanding on the mechanisms of TB root–microbe interactions. |
format | Online Article Text |
id | pubmed-10536138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105361382023-09-29 Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat Qiu, Qingcheng Xiang, Dabing Li, Qiang Wang, Hanlin Wan, Yan Wu, Qi Ye, Xueling Jiang, Liangzhen Fan, Yu Liu, Bingliang Liu, Yanxia Li, Han Liu, Changying Front Microbiol Microbiology Tartary buckwheat (Fagopyrum tataricum Gaertn.) is an important pseudocereal crop with excellent edible, nutritional and medicinal values. However, the yield of Tartary buckwheat (TB) is very low due to old-fashioned cultivation techniques, particularly unreasonable application of nitrogen fertilizer. To improve the understanding on the theories of nitrogen use in TB, the effects of nitrogen application on growth, as well as chemical properties and microbial community of rhizosphere soil were investigated in this study. Nitrogen application could promote the plant height, stem diameter, nitrogen accumulation and yield of TB. The relative abundance and diversity of bacteria and fungi in the rhizosphere soil of TB were improved by nitrogen fertilizer. Nitrogen application increased the abundance of beneficial bacteria such as Lysobacter and Sphingomonas in rhizosphere soil, and decreased the abundance of pathogenic fungi such as Fusarium and Plectosphaerella. The results indicated that nitrogen application changed the distribution of microbial communities in TB rhizosphere soil. Furthermore, the specific enriched or depleted microorganisms in the rhizosphere soil of four TB varieties were analyzed at OTU level. 87 specific nitrogen-responsive genes with sequence variation were identified in four varieties by integrating genomic re-sequencing and transcriptome analysis, and these genes may involve in the recruitment of specific rhizosphere microorganisms in different TB varieties. This study provided new insights into the effects of nitrogen application on TB growth and rhizosphere microbial community, and improved the understanding on the mechanisms of TB root–microbe interactions. Frontiers Media S.A. 2023-09-14 /pmc/articles/PMC10536138/ /pubmed/37779724 http://dx.doi.org/10.3389/fmicb.2023.1240029 Text en Copyright © 2023 Qiu, Xiang, Li, Wang, Wan, Wu, Ye, Jiang, Fan, Liu, Liu, Li and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Qiu, Qingcheng Xiang, Dabing Li, Qiang Wang, Hanlin Wan, Yan Wu, Qi Ye, Xueling Jiang, Liangzhen Fan, Yu Liu, Bingliang Liu, Yanxia Li, Han Liu, Changying Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat |
title | Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat |
title_full | Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat |
title_fullStr | Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat |
title_full_unstemmed | Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat |
title_short | Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat |
title_sort | interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of tartary buckwheat |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536138/ https://www.ncbi.nlm.nih.gov/pubmed/37779724 http://dx.doi.org/10.3389/fmicb.2023.1240029 |
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