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Portable Device for Potentiometric Determination of Antioxidant Capacity
For the first time, a prototype of a portable device for the potentiometric determination of antioxidant capacity based on a new measurement principle is proposed. A feature of the approach is the use of an electrochemical microcell with separated spaces and two identical electrodes with immobilized...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536404/ https://www.ncbi.nlm.nih.gov/pubmed/37765901 http://dx.doi.org/10.3390/s23187845 |
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author | Ivanova, Alla V. Markina, Maria G. |
author_facet | Ivanova, Alla V. Markina, Maria G. |
author_sort | Ivanova, Alla V. |
collection | PubMed |
description | For the first time, a prototype of a portable device for the potentiometric determination of antioxidant capacity based on a new measurement principle is proposed. A feature of the approach is the use of an electrochemical microcell with separated spaces and two identical electrodes with immobilized reagents. An antioxidant solution is introduced into one half-cell, and the antioxidants interact with the reagents. The other half-cell contains only reagents. The potential difference between the electrodes is due to the change in the ratio of the oxidized and reduced form of the reagents, which occurs as a result of the reaction with the antioxidants in one of the half-cells and is related to their concentration. The range of linearity of the microcell with immobilized reagents is 40–4000 μM-eq, and the limit of detection is 20 μM-eq. The device was successfully tested in the analysis of standard antioxidant solutions. The recoveries were (92–113)%, and the relative standard deviation did not exceed 15%. A good correlation was found between the data obtained by the approach and the potentiometric method in a macrocell for fruit juice analysis. Pearson’s coefficient for the obtained experimental data was 0.9955. The proposed portable device is promising and can be used in field conditions. |
format | Online Article Text |
id | pubmed-10536404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105364042023-09-29 Portable Device for Potentiometric Determination of Antioxidant Capacity Ivanova, Alla V. Markina, Maria G. Sensors (Basel) Article For the first time, a prototype of a portable device for the potentiometric determination of antioxidant capacity based on a new measurement principle is proposed. A feature of the approach is the use of an electrochemical microcell with separated spaces and two identical electrodes with immobilized reagents. An antioxidant solution is introduced into one half-cell, and the antioxidants interact with the reagents. The other half-cell contains only reagents. The potential difference between the electrodes is due to the change in the ratio of the oxidized and reduced form of the reagents, which occurs as a result of the reaction with the antioxidants in one of the half-cells and is related to their concentration. The range of linearity of the microcell with immobilized reagents is 40–4000 μM-eq, and the limit of detection is 20 μM-eq. The device was successfully tested in the analysis of standard antioxidant solutions. The recoveries were (92–113)%, and the relative standard deviation did not exceed 15%. A good correlation was found between the data obtained by the approach and the potentiometric method in a macrocell for fruit juice analysis. Pearson’s coefficient for the obtained experimental data was 0.9955. The proposed portable device is promising and can be used in field conditions. MDPI 2023-09-13 /pmc/articles/PMC10536404/ /pubmed/37765901 http://dx.doi.org/10.3390/s23187845 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ivanova, Alla V. Markina, Maria G. Portable Device for Potentiometric Determination of Antioxidant Capacity |
title | Portable Device for Potentiometric Determination of Antioxidant Capacity |
title_full | Portable Device for Potentiometric Determination of Antioxidant Capacity |
title_fullStr | Portable Device for Potentiometric Determination of Antioxidant Capacity |
title_full_unstemmed | Portable Device for Potentiometric Determination of Antioxidant Capacity |
title_short | Portable Device for Potentiometric Determination of Antioxidant Capacity |
title_sort | portable device for potentiometric determination of antioxidant capacity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536404/ https://www.ncbi.nlm.nih.gov/pubmed/37765901 http://dx.doi.org/10.3390/s23187845 |
work_keys_str_mv | AT ivanovaallav portabledeviceforpotentiometricdeterminationofantioxidantcapacity AT markinamariag portabledeviceforpotentiometricdeterminationofantioxidantcapacity |