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Portable Device for Potentiometric Determination of Antioxidant Capacity

For the first time, a prototype of a portable device for the potentiometric determination of antioxidant capacity based on a new measurement principle is proposed. A feature of the approach is the use of an electrochemical microcell with separated spaces and two identical electrodes with immobilized...

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Autores principales: Ivanova, Alla V., Markina, Maria G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536404/
https://www.ncbi.nlm.nih.gov/pubmed/37765901
http://dx.doi.org/10.3390/s23187845
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author Ivanova, Alla V.
Markina, Maria G.
author_facet Ivanova, Alla V.
Markina, Maria G.
author_sort Ivanova, Alla V.
collection PubMed
description For the first time, a prototype of a portable device for the potentiometric determination of antioxidant capacity based on a new measurement principle is proposed. A feature of the approach is the use of an electrochemical microcell with separated spaces and two identical electrodes with immobilized reagents. An antioxidant solution is introduced into one half-cell, and the antioxidants interact with the reagents. The other half-cell contains only reagents. The potential difference between the electrodes is due to the change in the ratio of the oxidized and reduced form of the reagents, which occurs as a result of the reaction with the antioxidants in one of the half-cells and is related to their concentration. The range of linearity of the microcell with immobilized reagents is 40–4000 μM-eq, and the limit of detection is 20 μM-eq. The device was successfully tested in the analysis of standard antioxidant solutions. The recoveries were (92–113)%, and the relative standard deviation did not exceed 15%. A good correlation was found between the data obtained by the approach and the potentiometric method in a macrocell for fruit juice analysis. Pearson’s coefficient for the obtained experimental data was 0.9955. The proposed portable device is promising and can be used in field conditions.
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spelling pubmed-105364042023-09-29 Portable Device for Potentiometric Determination of Antioxidant Capacity Ivanova, Alla V. Markina, Maria G. Sensors (Basel) Article For the first time, a prototype of a portable device for the potentiometric determination of antioxidant capacity based on a new measurement principle is proposed. A feature of the approach is the use of an electrochemical microcell with separated spaces and two identical electrodes with immobilized reagents. An antioxidant solution is introduced into one half-cell, and the antioxidants interact with the reagents. The other half-cell contains only reagents. The potential difference between the electrodes is due to the change in the ratio of the oxidized and reduced form of the reagents, which occurs as a result of the reaction with the antioxidants in one of the half-cells and is related to their concentration. The range of linearity of the microcell with immobilized reagents is 40–4000 μM-eq, and the limit of detection is 20 μM-eq. The device was successfully tested in the analysis of standard antioxidant solutions. The recoveries were (92–113)%, and the relative standard deviation did not exceed 15%. A good correlation was found between the data obtained by the approach and the potentiometric method in a macrocell for fruit juice analysis. Pearson’s coefficient for the obtained experimental data was 0.9955. The proposed portable device is promising and can be used in field conditions. MDPI 2023-09-13 /pmc/articles/PMC10536404/ /pubmed/37765901 http://dx.doi.org/10.3390/s23187845 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ivanova, Alla V.
Markina, Maria G.
Portable Device for Potentiometric Determination of Antioxidant Capacity
title Portable Device for Potentiometric Determination of Antioxidant Capacity
title_full Portable Device for Potentiometric Determination of Antioxidant Capacity
title_fullStr Portable Device for Potentiometric Determination of Antioxidant Capacity
title_full_unstemmed Portable Device for Potentiometric Determination of Antioxidant Capacity
title_short Portable Device for Potentiometric Determination of Antioxidant Capacity
title_sort portable device for potentiometric determination of antioxidant capacity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536404/
https://www.ncbi.nlm.nih.gov/pubmed/37765901
http://dx.doi.org/10.3390/s23187845
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