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Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis

Canine brucellosis caused by Brucella canis, is an infectious disease affecting dogs and wild Canidae. Clinical diagnosis is challenging, and laboratory testing is crucial for a definitive diagnosis. Various serological methods have been described, but their accuracy is uncertain due to limited vali...

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Autores principales: Perletta, Fabrizia, Di Pancrazio, Chiara, Rodomonti, Diamante, Di Febo, Tiziana, Luciani, Mirella, Krasteva, Ivanka Marinova, Maggetti, Marta, Profeta, Francesca, Salini, Romolo, De Massis, Fabrizio, Sacchini, Flavio, Tittarelli, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536495/
https://www.ncbi.nlm.nih.gov/pubmed/37764006
http://dx.doi.org/10.3390/microorganisms11092162
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author Perletta, Fabrizia
Di Pancrazio, Chiara
Rodomonti, Diamante
Di Febo, Tiziana
Luciani, Mirella
Krasteva, Ivanka Marinova
Maggetti, Marta
Profeta, Francesca
Salini, Romolo
De Massis, Fabrizio
Sacchini, Flavio
Tittarelli, Manuela
author_facet Perletta, Fabrizia
Di Pancrazio, Chiara
Rodomonti, Diamante
Di Febo, Tiziana
Luciani, Mirella
Krasteva, Ivanka Marinova
Maggetti, Marta
Profeta, Francesca
Salini, Romolo
De Massis, Fabrizio
Sacchini, Flavio
Tittarelli, Manuela
author_sort Perletta, Fabrizia
collection PubMed
description Canine brucellosis caused by Brucella canis, is an infectious disease affecting dogs and wild Canidae. Clinical diagnosis is challenging, and laboratory testing is crucial for a definitive diagnosis. Various serological methods have been described, but their accuracy is uncertain due to limited validation studies. The present study aimed to evaluate the performances of three serological tests for the diagnosis of B. canis in comparison with bacterial isolation (gold standard), in order to establish a protocol for the serological diagnosis of canine brucellosis. A panel of sera from naturally infected dogs (n = 61), from which B. canis was isolated, and uninfected dogs (n = 143), negative for B. canis isolation, were tested using microplate serum agglutination (mSAT), complement fixation performed using the Brucella ovis antigen (B. ovis-CFT), and a commercial immunofluorescence assay (IFAT). The sensitivity and specificity of the three serological methods were, respectively, the following: 96.7% (95% CI 88.8–98.7%) and 92.3 (95% CI 86.7–95.1%) for mSAT; 96.7% (95% CI 88.8–98.7%) and 96.5 (95% CI 92.1–98.2%) for B. ovis-CFT; 98.4% (95% CI 91.3–99.4%) and 99.3 (95% CI 96.2–99.8%) for IFAT. The use in of the three methods in parallel, combined with bacterial isolation and molecular methods, could improve the diagnosis of the infection in dogs.
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spelling pubmed-105364952023-09-29 Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis Perletta, Fabrizia Di Pancrazio, Chiara Rodomonti, Diamante Di Febo, Tiziana Luciani, Mirella Krasteva, Ivanka Marinova Maggetti, Marta Profeta, Francesca Salini, Romolo De Massis, Fabrizio Sacchini, Flavio Tittarelli, Manuela Microorganisms Article Canine brucellosis caused by Brucella canis, is an infectious disease affecting dogs and wild Canidae. Clinical diagnosis is challenging, and laboratory testing is crucial for a definitive diagnosis. Various serological methods have been described, but their accuracy is uncertain due to limited validation studies. The present study aimed to evaluate the performances of three serological tests for the diagnosis of B. canis in comparison with bacterial isolation (gold standard), in order to establish a protocol for the serological diagnosis of canine brucellosis. A panel of sera from naturally infected dogs (n = 61), from which B. canis was isolated, and uninfected dogs (n = 143), negative for B. canis isolation, were tested using microplate serum agglutination (mSAT), complement fixation performed using the Brucella ovis antigen (B. ovis-CFT), and a commercial immunofluorescence assay (IFAT). The sensitivity and specificity of the three serological methods were, respectively, the following: 96.7% (95% CI 88.8–98.7%) and 92.3 (95% CI 86.7–95.1%) for mSAT; 96.7% (95% CI 88.8–98.7%) and 96.5 (95% CI 92.1–98.2%) for B. ovis-CFT; 98.4% (95% CI 91.3–99.4%) and 99.3 (95% CI 96.2–99.8%) for IFAT. The use in of the three methods in parallel, combined with bacterial isolation and molecular methods, could improve the diagnosis of the infection in dogs. MDPI 2023-08-26 /pmc/articles/PMC10536495/ /pubmed/37764006 http://dx.doi.org/10.3390/microorganisms11092162 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perletta, Fabrizia
Di Pancrazio, Chiara
Rodomonti, Diamante
Di Febo, Tiziana
Luciani, Mirella
Krasteva, Ivanka Marinova
Maggetti, Marta
Profeta, Francesca
Salini, Romolo
De Massis, Fabrizio
Sacchini, Flavio
Tittarelli, Manuela
Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis
title Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis
title_full Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis
title_fullStr Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis
title_full_unstemmed Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis
title_short Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis
title_sort evaluation of three serological tests for diagnosis of canine brucellosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536495/
https://www.ncbi.nlm.nih.gov/pubmed/37764006
http://dx.doi.org/10.3390/microorganisms11092162
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