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Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein

Nairobi sheep disease (NSD), caused by the viral agent NSD virus (NSDV), is a haemorrhagic fever disease affecting and inducing high mortality in sheep and goat populations. NSDV belongs to the genus Orthonairovirus of the Nairoviridae family from the order Bunyavirales. Other viruses circulating in...

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Autores principales: Maze, Emmanuel A., Chrun, Tiphany, Booth, George, Limon, Georgina, Charleston, Bryan, Lambe, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536980/
https://www.ncbi.nlm.nih.gov/pubmed/37766282
http://dx.doi.org/10.3390/v15091876
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author Maze, Emmanuel A.
Chrun, Tiphany
Booth, George
Limon, Georgina
Charleston, Bryan
Lambe, Teresa
author_facet Maze, Emmanuel A.
Chrun, Tiphany
Booth, George
Limon, Georgina
Charleston, Bryan
Lambe, Teresa
author_sort Maze, Emmanuel A.
collection PubMed
description Nairobi sheep disease (NSD), caused by the viral agent NSD virus (NSDV), is a haemorrhagic fever disease affecting and inducing high mortality in sheep and goat populations. NSDV belongs to the genus Orthonairovirus of the Nairoviridae family from the order Bunyavirales. Other viruses circulating in livestock such as Crimean–Congo haemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV) are members of the same genus and are reported to share antigenic features. There are very few available materials to study NSDV infection both in vitro and in vivo. In the present work, we characterised two monoclonal antibodies generated in mice that recognise NSDV specifically but not CCHFV or DUGV, along with a potential use to define virus-infected cells, using flow cytometry. We believe this tool can be useful for research, but also NSDV diagnostics, especially through immunological staining.
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spelling pubmed-105369802023-09-29 Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein Maze, Emmanuel A. Chrun, Tiphany Booth, George Limon, Georgina Charleston, Bryan Lambe, Teresa Viruses Brief Report Nairobi sheep disease (NSD), caused by the viral agent NSD virus (NSDV), is a haemorrhagic fever disease affecting and inducing high mortality in sheep and goat populations. NSDV belongs to the genus Orthonairovirus of the Nairoviridae family from the order Bunyavirales. Other viruses circulating in livestock such as Crimean–Congo haemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV) are members of the same genus and are reported to share antigenic features. There are very few available materials to study NSDV infection both in vitro and in vivo. In the present work, we characterised two monoclonal antibodies generated in mice that recognise NSDV specifically but not CCHFV or DUGV, along with a potential use to define virus-infected cells, using flow cytometry. We believe this tool can be useful for research, but also NSDV diagnostics, especially through immunological staining. MDPI 2023-09-05 /pmc/articles/PMC10536980/ /pubmed/37766282 http://dx.doi.org/10.3390/v15091876 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Maze, Emmanuel A.
Chrun, Tiphany
Booth, George
Limon, Georgina
Charleston, Bryan
Lambe, Teresa
Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein
title Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein
title_full Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein
title_fullStr Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein
title_full_unstemmed Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein
title_short Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein
title_sort generation and characterisation of monoclonal antibodies against nairobi sheep disease virus nucleoprotein
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536980/
https://www.ncbi.nlm.nih.gov/pubmed/37766282
http://dx.doi.org/10.3390/v15091876
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