Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools

The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive...

Descripción completa

Detalles Bibliográficos
Autores principales: Hider, Robert Charles, Pourzand, Charareh, Ma, Yongmin, Cilibrizzi, Agostino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537325/
https://www.ncbi.nlm.nih.gov/pubmed/37764245
http://dx.doi.org/10.3390/molecules28186467
_version_ 1785113076183334912
author Hider, Robert Charles
Pourzand, Charareh
Ma, Yongmin
Cilibrizzi, Agostino
author_facet Hider, Robert Charles
Pourzand, Charareh
Ma, Yongmin
Cilibrizzi, Agostino
author_sort Hider, Robert Charles
collection PubMed
description The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed.
format Online
Article
Text
id pubmed-10537325
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105373252023-09-29 Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools Hider, Robert Charles Pourzand, Charareh Ma, Yongmin Cilibrizzi, Agostino Molecules Review The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed. MDPI 2023-09-06 /pmc/articles/PMC10537325/ /pubmed/37764245 http://dx.doi.org/10.3390/molecules28186467 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Hider, Robert Charles
Pourzand, Charareh
Ma, Yongmin
Cilibrizzi, Agostino
Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools
title Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools
title_full Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools
title_fullStr Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools
title_full_unstemmed Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools
title_short Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools
title_sort optical imaging opportunities to inspect the nature of cytosolic iron pools
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537325/
https://www.ncbi.nlm.nih.gov/pubmed/37764245
http://dx.doi.org/10.3390/molecules28186467
work_keys_str_mv AT hiderrobertcharles opticalimagingopportunitiestoinspectthenatureofcytosolicironpools
AT pourzandcharareh opticalimagingopportunitiestoinspectthenatureofcytosolicironpools
AT mayongmin opticalimagingopportunitiestoinspectthenatureofcytosolicironpools
AT cilibrizziagostino opticalimagingopportunitiestoinspectthenatureofcytosolicironpools

Ejemplares similares