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First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece

The objective of the present study is to report the detection and the molecular characterization of nine bla(NDM-1)-positive Pseudomonas aeruginosa isolates, all of which belonged to the epidemic high-risk international clone ST308, and all were isolated from patients in a tertiary care hospital in...

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Autores principales: Tsilipounidaki, Katerina, Gkountinoudis, Christos-George, Florou, Zoi, Fthenakis, George C., Miriagou, Vivi, Petinaki, Efthymia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537375/
https://www.ncbi.nlm.nih.gov/pubmed/37764003
http://dx.doi.org/10.3390/microorganisms11092159
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author Tsilipounidaki, Katerina
Gkountinoudis, Christos-George
Florou, Zoi
Fthenakis, George C.
Miriagou, Vivi
Petinaki, Efthymia
author_facet Tsilipounidaki, Katerina
Gkountinoudis, Christos-George
Florou, Zoi
Fthenakis, George C.
Miriagou, Vivi
Petinaki, Efthymia
author_sort Tsilipounidaki, Katerina
collection PubMed
description The objective of the present study is to report the detection and the molecular characterization of nine bla(NDM-1)-positive Pseudomonas aeruginosa isolates, all of which belonged to the epidemic high-risk international clone ST308, and all were isolated from patients in a tertiary care hospital in Central Greece from May to July 2023.The isolates were characterized by whole genome sequencing to obtain multi-locus sequencing typing (MLST) and identify the bla(NDM1)-environment and resistome and virulence genes content. In silico MLST analysis showed that all isolates belonged to the high-risk ST308 international clone. All strains possessed 22 different genes, encoding resistance to various antimicrobial agents. Whole genome sequencing revealed that the bla(NDM-1) was chromosomally located within the integrative and conjugative element ICE(Tn4371)6385 and that it was part of one cassette along with two other resistance genes, floR and msrE. Two additional resistance cassettes were also found in the genome, which included the arrays of aph(6)-Id, aph(3″)-Ib, floR, sul2 and aadA10, qnrVC1, aac(3)-Id, dfrB5, aac(6′)-II. Additionally, the strains possessed various virulence genes, e.g., aprA, exoU, lasA, lasB, toxA, and estA. All of the isolates shared identical genomes, which showed 98% similarity with the P. aeruginosa ST308 genome (acc. no CP020703), previously reported from Singapore. To our knowledge, this is the first report of ST308 bla(NDM-1)-positive P. aeruginosa isolation in Europe, which indicates the transmission dynamics of this high-risk clone.
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spelling pubmed-105373752023-09-29 First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece Tsilipounidaki, Katerina Gkountinoudis, Christos-George Florou, Zoi Fthenakis, George C. Miriagou, Vivi Petinaki, Efthymia Microorganisms Communication The objective of the present study is to report the detection and the molecular characterization of nine bla(NDM-1)-positive Pseudomonas aeruginosa isolates, all of which belonged to the epidemic high-risk international clone ST308, and all were isolated from patients in a tertiary care hospital in Central Greece from May to July 2023.The isolates were characterized by whole genome sequencing to obtain multi-locus sequencing typing (MLST) and identify the bla(NDM1)-environment and resistome and virulence genes content. In silico MLST analysis showed that all isolates belonged to the high-risk ST308 international clone. All strains possessed 22 different genes, encoding resistance to various antimicrobial agents. Whole genome sequencing revealed that the bla(NDM-1) was chromosomally located within the integrative and conjugative element ICE(Tn4371)6385 and that it was part of one cassette along with two other resistance genes, floR and msrE. Two additional resistance cassettes were also found in the genome, which included the arrays of aph(6)-Id, aph(3″)-Ib, floR, sul2 and aadA10, qnrVC1, aac(3)-Id, dfrB5, aac(6′)-II. Additionally, the strains possessed various virulence genes, e.g., aprA, exoU, lasA, lasB, toxA, and estA. All of the isolates shared identical genomes, which showed 98% similarity with the P. aeruginosa ST308 genome (acc. no CP020703), previously reported from Singapore. To our knowledge, this is the first report of ST308 bla(NDM-1)-positive P. aeruginosa isolation in Europe, which indicates the transmission dynamics of this high-risk clone. MDPI 2023-08-26 /pmc/articles/PMC10537375/ /pubmed/37764003 http://dx.doi.org/10.3390/microorganisms11092159 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Tsilipounidaki, Katerina
Gkountinoudis, Christos-George
Florou, Zoi
Fthenakis, George C.
Miriagou, Vivi
Petinaki, Efthymia
First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece
title First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece
title_full First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece
title_fullStr First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece
title_full_unstemmed First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece
title_short First Detection and Molecular Characterization of Pseudomonas aeruginosa bla(NDM-1) ST308 in Greece
title_sort first detection and molecular characterization of pseudomonas aeruginosa bla(ndm-1) st308 in greece
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537375/
https://www.ncbi.nlm.nih.gov/pubmed/37764003
http://dx.doi.org/10.3390/microorganisms11092159
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