Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique

A preliminary exploration of the physiology and morphology of the zebrafish embryo (ZFE) during the late-blastula and early-gastrula stages through its electrical properties was performed, applying the electrorotation (ROT) technique. This method, based on induced polarizability at the interfaces, w...

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Autores principales: Sanchis-Otero, Aránzazu, Reina-Maldonado, María Teresa, Roldán, José, Barragán, Vicenta María, Muñoz, Sagrario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537415/
https://www.ncbi.nlm.nih.gov/pubmed/37755207
http://dx.doi.org/10.3390/membranes13090785
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author Sanchis-Otero, Aránzazu
Reina-Maldonado, María Teresa
Roldán, José
Barragán, Vicenta María
Muñoz, Sagrario
author_facet Sanchis-Otero, Aránzazu
Reina-Maldonado, María Teresa
Roldán, José
Barragán, Vicenta María
Muñoz, Sagrario
author_sort Sanchis-Otero, Aránzazu
collection PubMed
description A preliminary exploration of the physiology and morphology of the zebrafish embryo (ZFE) during the late-blastula and early-gastrula stages through its electrical properties was performed, applying the electrorotation (ROT) technique. This method, based on induced polarizability at the interfaces, was combined with an analytical spherical shell model to obtain the best fit of empirical data and the desired information, providing a means of understanding the role of different membranes. Suspended in two solutions of low conductivity, the major compartments of the ZFE were electrically characterized, considering morphological data from both observed records and data from the literature. Membrane integrity was also analyzed for dead embryos. The low permeability and relatively high permittivity obtained for the chorion probably reflected both its structural characteristics and external conditions. Reasonable values were derived for perivitelline fluid according to the influx of water that occurs after the fertilization of the oocyte. The so-called yolk membrane, which comprises three different and contiguous layers at the epiboly stage, showed atypical electrical values of the membrane, as did the yolk core with a relatively low permittivity. The internal morphological complexity of the embryo itself could be addressed in future studies by developing an accurate geometric model.
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spelling pubmed-105374152023-09-29 Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique Sanchis-Otero, Aránzazu Reina-Maldonado, María Teresa Roldán, José Barragán, Vicenta María Muñoz, Sagrario Membranes (Basel) Article A preliminary exploration of the physiology and morphology of the zebrafish embryo (ZFE) during the late-blastula and early-gastrula stages through its electrical properties was performed, applying the electrorotation (ROT) technique. This method, based on induced polarizability at the interfaces, was combined with an analytical spherical shell model to obtain the best fit of empirical data and the desired information, providing a means of understanding the role of different membranes. Suspended in two solutions of low conductivity, the major compartments of the ZFE were electrically characterized, considering morphological data from both observed records and data from the literature. Membrane integrity was also analyzed for dead embryos. The low permeability and relatively high permittivity obtained for the chorion probably reflected both its structural characteristics and external conditions. Reasonable values were derived for perivitelline fluid according to the influx of water that occurs after the fertilization of the oocyte. The so-called yolk membrane, which comprises three different and contiguous layers at the epiboly stage, showed atypical electrical values of the membrane, as did the yolk core with a relatively low permittivity. The internal morphological complexity of the embryo itself could be addressed in future studies by developing an accurate geometric model. MDPI 2023-09-09 /pmc/articles/PMC10537415/ /pubmed/37755207 http://dx.doi.org/10.3390/membranes13090785 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sanchis-Otero, Aránzazu
Reina-Maldonado, María Teresa
Roldán, José
Barragán, Vicenta María
Muñoz, Sagrario
Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique
title Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique
title_full Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique
title_fullStr Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique
title_full_unstemmed Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique
title_short Investigation of Zebrafish Embryo Membranes at Epiboly Stage through Electrorotation Technique
title_sort investigation of zebrafish embryo membranes at epiboly stage through electrorotation technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537415/
https://www.ncbi.nlm.nih.gov/pubmed/37755207
http://dx.doi.org/10.3390/membranes13090785
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