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Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane

In this study, a polyacrylonitrile nanofiber membrane was first hydrolyzed and then functionalized with tris(hydroxymethyl)aminomethane (P-Tris), then used as an affinity nanofiber membrane for lysozyme adsorption in membrane chromatography. The dynamic adsorption behavior of lysozyme was investigat...

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Autores principales: Chen, Kuei-Hsiang, Lai, You-Ren, Hanh, Nguyen The Duc, Wang, Steven S.-S., Chang, Yu-Kaung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537428/
https://www.ncbi.nlm.nih.gov/pubmed/37755183
http://dx.doi.org/10.3390/membranes13090761
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author Chen, Kuei-Hsiang
Lai, You-Ren
Hanh, Nguyen The Duc
Wang, Steven S.-S.
Chang, Yu-Kaung
author_facet Chen, Kuei-Hsiang
Lai, You-Ren
Hanh, Nguyen The Duc
Wang, Steven S.-S.
Chang, Yu-Kaung
author_sort Chen, Kuei-Hsiang
collection PubMed
description In this study, a polyacrylonitrile nanofiber membrane was first hydrolyzed and then functionalized with tris(hydroxymethyl)aminomethane (P-Tris), then used as an affinity nanofiber membrane for lysozyme adsorption in membrane chromatography. The dynamic adsorption behavior of lysozyme was investigated in a flow system under various operating parameters, including adsorption pHs, initial feed lysozyme concentration, loading flow rate, and the number of stacked membrane layers. Four different kinetic models, pseudo-first-order, pseudo-second-order, Elovich, and intraparticle diffusion kinetic models, were applied to experimental data from breakthrough curves of lysozyme. The results showed that the dynamic adsorption results were fitted well with the pseudo-second-order kinetic model. The breakthrough curve experimental results show significant differences in the breakthrough time, the dynamic binding capacity, the length of the mass transfer zone, and the utilization rate of the membrane bed under different operating parameters. Four dynamic adsorption models (i.e., Bohart–Adams, Thomas, Yoon–Nelson, and BDST models) were used to analyze the breakthrough curve characteristics of the dynamic adsorption experiments. Among them, the Yoon–Nelson model was the best model to fit the breakthrough curve. However, some of the theoretical results based on the Thomas and Bohart–Adams model analyses of the breakthrough curve fit well with the experimental data, with an error percentage of <5%. The Bohart–Adams model has the largest difference from the experimental results; hence it is not suitable for breakthrough curve analysis. These results significantly impact dynamic kinetics studies and breakthrough curve characteristic analysis in membrane bed chromatography.
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spelling pubmed-105374282023-09-29 Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane Chen, Kuei-Hsiang Lai, You-Ren Hanh, Nguyen The Duc Wang, Steven S.-S. Chang, Yu-Kaung Membranes (Basel) Article In this study, a polyacrylonitrile nanofiber membrane was first hydrolyzed and then functionalized with tris(hydroxymethyl)aminomethane (P-Tris), then used as an affinity nanofiber membrane for lysozyme adsorption in membrane chromatography. The dynamic adsorption behavior of lysozyme was investigated in a flow system under various operating parameters, including adsorption pHs, initial feed lysozyme concentration, loading flow rate, and the number of stacked membrane layers. Four different kinetic models, pseudo-first-order, pseudo-second-order, Elovich, and intraparticle diffusion kinetic models, were applied to experimental data from breakthrough curves of lysozyme. The results showed that the dynamic adsorption results were fitted well with the pseudo-second-order kinetic model. The breakthrough curve experimental results show significant differences in the breakthrough time, the dynamic binding capacity, the length of the mass transfer zone, and the utilization rate of the membrane bed under different operating parameters. Four dynamic adsorption models (i.e., Bohart–Adams, Thomas, Yoon–Nelson, and BDST models) were used to analyze the breakthrough curve characteristics of the dynamic adsorption experiments. Among them, the Yoon–Nelson model was the best model to fit the breakthrough curve. However, some of the theoretical results based on the Thomas and Bohart–Adams model analyses of the breakthrough curve fit well with the experimental data, with an error percentage of <5%. The Bohart–Adams model has the largest difference from the experimental results; hence it is not suitable for breakthrough curve analysis. These results significantly impact dynamic kinetics studies and breakthrough curve characteristic analysis in membrane bed chromatography. MDPI 2023-08-28 /pmc/articles/PMC10537428/ /pubmed/37755183 http://dx.doi.org/10.3390/membranes13090761 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Kuei-Hsiang
Lai, You-Ren
Hanh, Nguyen The Duc
Wang, Steven S.-S.
Chang, Yu-Kaung
Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane
title Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane
title_full Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane
title_fullStr Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane
title_full_unstemmed Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane
title_short Breakthrough Curve Modeling and Analysis for Lysozyme Adsorption by Tris(hydroxymethyl)aminomethane Affinity Nanofiber Membrane
title_sort breakthrough curve modeling and analysis for lysozyme adsorption by tris(hydroxymethyl)aminomethane affinity nanofiber membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537428/
https://www.ncbi.nlm.nih.gov/pubmed/37755183
http://dx.doi.org/10.3390/membranes13090761
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