Cargando…
Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study
BACKGROUND: Recent research has established the correlation between gut microbiota and periodontitis via oral-gut axis. Intestinal dysbiosis may play a pivotal bridging role in extra-oral inflammatory comorbidities caused by periodontitis. However, it is unclear whether the link is merely correlativ...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537583/ https://www.ncbi.nlm.nih.gov/pubmed/37770955 http://dx.doi.org/10.1186/s12967-023-04559-9 |
_version_ | 1785113135895543808 |
---|---|
author | Ye, Xinjian Liu, Bin Bai, Yijing Cao, Yue Lin, Sirui Lyu, Linshuoshuo Meng, Haohao Dai, Yuwei Ye, Ding Pan, Weiyi Wang, Zhiyong Mao, Yingying Chen, Qianming |
author_facet | Ye, Xinjian Liu, Bin Bai, Yijing Cao, Yue Lin, Sirui Lyu, Linshuoshuo Meng, Haohao Dai, Yuwei Ye, Ding Pan, Weiyi Wang, Zhiyong Mao, Yingying Chen, Qianming |
author_sort | Ye, Xinjian |
collection | PubMed |
description | BACKGROUND: Recent research has established the correlation between gut microbiota and periodontitis via oral-gut axis. Intestinal dysbiosis may play a pivotal bridging role in extra-oral inflammatory comorbidities caused by periodontitis. However, it is unclear whether the link is merely correlative or orchestrated by causative mechanistic interactions. This two-sample Mendelian randomization (MR) study was performed to evaluate the potential bidirectional causal relationships between gut microbiota and periodontitis. MATERIALS AND METHODS: A two-sample MR analysis was performed using summary statistics from genome-wide association studies (GWAS) for gut microbiota (n = 18,340) and periodontitis (cases = 12,251; controls = 22,845). The inverse-variance weighted (IVW) method was used for the primary analysis, and we employed sensitivity analyses to assess the robustness of the main results. The PhenoScanner database was then searched for pleiotropy SNPs associated with potential confounders. In order to identify the possibly influential SNPs, we further conducted the leave-one-out analysis. Finally, a reverse MR analysis was performed to evaluate the possibility of links between periodontitis and genetically predicted gut microbiota alternation. RESULTS: 2,699 single nucleotide polymorphisms (SNPs) associated with 196 microbiota genera were selected as instrumental variables (IVs). IVW method suggested that order Enterobacteriales (OR: 1.35, 95% CI 1.10–1.66), family Bacteroidales S24.7group (OR: 1.22, 95% CI 1.05–1.41), genus Lachnospiraceae UCG008 (OR: 1.16, 95% CI 1.03–1.31), genus Prevotella 7 (OR: 1.11, 95% CI 1.01–1.23), and order Pasteurellales (OR: 1.12, 95% CI 1.00–1.26) may be associated with a higher risk of periodontitis, while genus Ruminiclostridium 6 may be linked to a lower risk (OR: 0.82, 95% CI 0.70–0.95). The sensitivity and heterogeneity analyses yielded no indication of horizontal pleiotropy or heterogeneity. Only the association between order Enterobacteriales and the likelihood of periodontitis remained consistent across all alternative MR approaches. In the reverse MR analysis, four microbiota genera were genetically predicted to be down-regulated in periodontitis, whereas two were predicted to be up-regulated. CONCLUSIONS: The present MR analysis demonstrated the potential bidirectional causal relationships between gut microbiota and periodontitis. Our research provided fresh insights for the prevention and management of periodontitis. Future research is required to support the finding of our current study. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-023-04559-9. |
format | Online Article Text |
id | pubmed-10537583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105375832023-09-29 Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study Ye, Xinjian Liu, Bin Bai, Yijing Cao, Yue Lin, Sirui Lyu, Linshuoshuo Meng, Haohao Dai, Yuwei Ye, Ding Pan, Weiyi Wang, Zhiyong Mao, Yingying Chen, Qianming J Transl Med Research BACKGROUND: Recent research has established the correlation between gut microbiota and periodontitis via oral-gut axis. Intestinal dysbiosis may play a pivotal bridging role in extra-oral inflammatory comorbidities caused by periodontitis. However, it is unclear whether the link is merely correlative or orchestrated by causative mechanistic interactions. This two-sample Mendelian randomization (MR) study was performed to evaluate the potential bidirectional causal relationships between gut microbiota and periodontitis. MATERIALS AND METHODS: A two-sample MR analysis was performed using summary statistics from genome-wide association studies (GWAS) for gut microbiota (n = 18,340) and periodontitis (cases = 12,251; controls = 22,845). The inverse-variance weighted (IVW) method was used for the primary analysis, and we employed sensitivity analyses to assess the robustness of the main results. The PhenoScanner database was then searched for pleiotropy SNPs associated with potential confounders. In order to identify the possibly influential SNPs, we further conducted the leave-one-out analysis. Finally, a reverse MR analysis was performed to evaluate the possibility of links between periodontitis and genetically predicted gut microbiota alternation. RESULTS: 2,699 single nucleotide polymorphisms (SNPs) associated with 196 microbiota genera were selected as instrumental variables (IVs). IVW method suggested that order Enterobacteriales (OR: 1.35, 95% CI 1.10–1.66), family Bacteroidales S24.7group (OR: 1.22, 95% CI 1.05–1.41), genus Lachnospiraceae UCG008 (OR: 1.16, 95% CI 1.03–1.31), genus Prevotella 7 (OR: 1.11, 95% CI 1.01–1.23), and order Pasteurellales (OR: 1.12, 95% CI 1.00–1.26) may be associated with a higher risk of periodontitis, while genus Ruminiclostridium 6 may be linked to a lower risk (OR: 0.82, 95% CI 0.70–0.95). The sensitivity and heterogeneity analyses yielded no indication of horizontal pleiotropy or heterogeneity. Only the association between order Enterobacteriales and the likelihood of periodontitis remained consistent across all alternative MR approaches. In the reverse MR analysis, four microbiota genera were genetically predicted to be down-regulated in periodontitis, whereas two were predicted to be up-regulated. CONCLUSIONS: The present MR analysis demonstrated the potential bidirectional causal relationships between gut microbiota and periodontitis. Our research provided fresh insights for the prevention and management of periodontitis. Future research is required to support the finding of our current study. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-023-04559-9. BioMed Central 2023-09-28 /pmc/articles/PMC10537583/ /pubmed/37770955 http://dx.doi.org/10.1186/s12967-023-04559-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ye, Xinjian Liu, Bin Bai, Yijing Cao, Yue Lin, Sirui Lyu, Linshuoshuo Meng, Haohao Dai, Yuwei Ye, Ding Pan, Weiyi Wang, Zhiyong Mao, Yingying Chen, Qianming Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study |
title | Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study |
title_full | Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study |
title_fullStr | Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study |
title_full_unstemmed | Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study |
title_short | Genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample Mendelian randomization study |
title_sort | genetic evidence strengthens the bidirectional connection between gut microbiota and periodontitis: insights from a two-sample mendelian randomization study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537583/ https://www.ncbi.nlm.nih.gov/pubmed/37770955 http://dx.doi.org/10.1186/s12967-023-04559-9 |
work_keys_str_mv | AT yexinjian geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT liubin geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT baiyijing geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT caoyue geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT linsirui geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT lyulinshuoshuo geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT menghaohao geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT daiyuwei geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT yeding geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT panweiyi geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT wangzhiyong geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT maoyingying geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy AT chenqianming geneticevidencestrengthensthebidirectionalconnectionbetweengutmicrobiotaandperiodontitisinsightsfromatwosamplemendelianrandomizationstudy |