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Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells

An aqueous extract of Syzygium cumini seeds was utilized to green synthesize titanium dioxide nanoparticles (TiO(2) NPs). UV-Visible, DLS, FTIR, XRD, FESEM, TEM, SAED, EDAX, and photoluminescence spectroscopy techniques were employed to characterize the prepared TiO(2) nanoparticles. The rutile crys...

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Autores principales: Rayzah, Musaed, Elderdery, Abozer Y., Alzerwi, Nasser A. N., Alzahrani, Badr, Alsrhani, Abdullah, Alsultan, Afnan, Idrees, Bandar, Rayzah, Fares, Bakhsh, Yaser, Alzahrani, Ahmed M., Subbiah, Suresh K., Mok, Pooi Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537597/
https://www.ncbi.nlm.nih.gov/pubmed/37765338
http://dx.doi.org/10.3390/plants12183174
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author Rayzah, Musaed
Elderdery, Abozer Y.
Alzerwi, Nasser A. N.
Alzahrani, Badr
Alsrhani, Abdullah
Alsultan, Afnan
Idrees, Bandar
Rayzah, Fares
Bakhsh, Yaser
Alzahrani, Ahmed M.
Subbiah, Suresh K.
Mok, Pooi Ling
author_facet Rayzah, Musaed
Elderdery, Abozer Y.
Alzerwi, Nasser A. N.
Alzahrani, Badr
Alsrhani, Abdullah
Alsultan, Afnan
Idrees, Bandar
Rayzah, Fares
Bakhsh, Yaser
Alzahrani, Ahmed M.
Subbiah, Suresh K.
Mok, Pooi Ling
author_sort Rayzah, Musaed
collection PubMed
description An aqueous extract of Syzygium cumini seeds was utilized to green synthesize titanium dioxide nanoparticles (TiO(2) NPs). UV-Visible, DLS, FTIR, XRD, FESEM, TEM, SAED, EDAX, and photoluminescence spectroscopy techniques were employed to characterize the prepared TiO(2) nanoparticles. The rutile crystal structure of TiO(2) NPs was revealed by XRD study. The TEM and FESEM images of the TiO(2) NPs revealed an average particle size of 50–100 nm. We employed EDAX to investigate the elemental compositions of TiO(2) NPs. The O-Ti-O stretching bands appeared in the FTIR spectrum of TiO(2) NPs at wavenumbers of 495 cm(−1). The absorption edge peaks of TiO(2) NPs were found in the UV-vis spectra at 397 nm. The MTT study revealed that TiO(2) NPs effectively inhibited the growth of liver cancer Hep3 and Hep-G2 cells. The results of the corresponding fluorescent staining assays showed that TiO(2) NPs significantly increased ROS generation, decreased MMP, and induced apoptosis in both liver cancer Hep3 and Hep-G2 cells. TiO(2) nanoparticles lessened SOD, CAT, and GSH levels while augmenting MDA contents in Hep3 and Hep-G2 cells. In both Hep3 and Hep-G2 cells treated with TiO(2) NPs, the Bax, CytC, p53, caspase-3, -8, and -9 expressions were remarkably augmented, while Bcl-2 expression was reduced. Overall, these findings revealed that formulated TiO(2) NPs treatment considerably inhibited growth and triggered apoptosis in Hep3 and HepG2 cells.
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spelling pubmed-105375972023-09-29 Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells Rayzah, Musaed Elderdery, Abozer Y. Alzerwi, Nasser A. N. Alzahrani, Badr Alsrhani, Abdullah Alsultan, Afnan Idrees, Bandar Rayzah, Fares Bakhsh, Yaser Alzahrani, Ahmed M. Subbiah, Suresh K. Mok, Pooi Ling Plants (Basel) Article An aqueous extract of Syzygium cumini seeds was utilized to green synthesize titanium dioxide nanoparticles (TiO(2) NPs). UV-Visible, DLS, FTIR, XRD, FESEM, TEM, SAED, EDAX, and photoluminescence spectroscopy techniques were employed to characterize the prepared TiO(2) nanoparticles. The rutile crystal structure of TiO(2) NPs was revealed by XRD study. The TEM and FESEM images of the TiO(2) NPs revealed an average particle size of 50–100 nm. We employed EDAX to investigate the elemental compositions of TiO(2) NPs. The O-Ti-O stretching bands appeared in the FTIR spectrum of TiO(2) NPs at wavenumbers of 495 cm(−1). The absorption edge peaks of TiO(2) NPs were found in the UV-vis spectra at 397 nm. The MTT study revealed that TiO(2) NPs effectively inhibited the growth of liver cancer Hep3 and Hep-G2 cells. The results of the corresponding fluorescent staining assays showed that TiO(2) NPs significantly increased ROS generation, decreased MMP, and induced apoptosis in both liver cancer Hep3 and Hep-G2 cells. TiO(2) nanoparticles lessened SOD, CAT, and GSH levels while augmenting MDA contents in Hep3 and Hep-G2 cells. In both Hep3 and Hep-G2 cells treated with TiO(2) NPs, the Bax, CytC, p53, caspase-3, -8, and -9 expressions were remarkably augmented, while Bcl-2 expression was reduced. Overall, these findings revealed that formulated TiO(2) NPs treatment considerably inhibited growth and triggered apoptosis in Hep3 and HepG2 cells. MDPI 2023-09-05 /pmc/articles/PMC10537597/ /pubmed/37765338 http://dx.doi.org/10.3390/plants12183174 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rayzah, Musaed
Elderdery, Abozer Y.
Alzerwi, Nasser A. N.
Alzahrani, Badr
Alsrhani, Abdullah
Alsultan, Afnan
Idrees, Bandar
Rayzah, Fares
Bakhsh, Yaser
Alzahrani, Ahmed M.
Subbiah, Suresh K.
Mok, Pooi Ling
Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells
title Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells
title_full Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells
title_fullStr Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells
title_full_unstemmed Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells
title_short Syzygium cumini (L.) Extract-Derived Green Titanium Dioxide Nanoparticles Induce Caspase-Dependent Apoptosis in Hepatic Cancer Cells
title_sort syzygium cumini (l.) extract-derived green titanium dioxide nanoparticles induce caspase-dependent apoptosis in hepatic cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537597/
https://www.ncbi.nlm.nih.gov/pubmed/37765338
http://dx.doi.org/10.3390/plants12183174
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