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Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia

Bacteria, designated as A1.1 and A1.2, were isolated from poultry waste based on the ability to form ammonia on LB nutrient medium. Whole genome sequencing identified the studied strains as Peribacillus frigoritolerans VKM B-3700D (A1.1) and Bacillus subtilis VKM B-3701D (A1.2) with genome sizes of...

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Autores principales: Senchenkov, Vladislav Yu., Lyakhovchenko, Nikita S., Nikishin, Ilya A., Myagkov, Dmitry A., Chepurina, Anna A., Polivtseva, Valentina N., Abashina, Tatiana N., Delegan, Yanina A., Nikulicheva, Tatiana B., Nikulin, Ivan S., Bogun, Alexander G., Solomentsev, Viktor I., Solyanikova, Inna P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537646/
https://www.ncbi.nlm.nih.gov/pubmed/37764079
http://dx.doi.org/10.3390/microorganisms11092235
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author Senchenkov, Vladislav Yu.
Lyakhovchenko, Nikita S.
Nikishin, Ilya A.
Myagkov, Dmitry A.
Chepurina, Anna A.
Polivtseva, Valentina N.
Abashina, Tatiana N.
Delegan, Yanina A.
Nikulicheva, Tatiana B.
Nikulin, Ivan S.
Bogun, Alexander G.
Solomentsev, Viktor I.
Solyanikova, Inna P.
author_facet Senchenkov, Vladislav Yu.
Lyakhovchenko, Nikita S.
Nikishin, Ilya A.
Myagkov, Dmitry A.
Chepurina, Anna A.
Polivtseva, Valentina N.
Abashina, Tatiana N.
Delegan, Yanina A.
Nikulicheva, Tatiana B.
Nikulin, Ivan S.
Bogun, Alexander G.
Solomentsev, Viktor I.
Solyanikova, Inna P.
author_sort Senchenkov, Vladislav Yu.
collection PubMed
description Bacteria, designated as A1.1 and A1.2, were isolated from poultry waste based on the ability to form ammonia on LB nutrient medium. Whole genome sequencing identified the studied strains as Peribacillus frigoritolerans VKM B-3700D (A1.1) and Bacillus subtilis VKM B-3701D (A1.2) with genome sizes of 5462638 and 4158287 bp, respectively. In the genome of B. subtilis VKM B-3701D, gene clusters of secondary metabolites of bacillin, subtilisin, bacilisin, surfactin, bacilliacin, fengycin, sactipeptide, and ratipeptide (spore killing factor) with potential antimicrobial activity were identified. Clusters of coronimine and peninodin production genes were found in P. frigoritolerans VKM B-3700D. Information on coronimine in bacteria is extremely limited. The study of the individual properties of the strains showed that the cultures are capable of biosynthesis of a number of enzymes, including amylases. The B. subtilis VKM V-3701D inhibited the growth of bacterial test cultures and reduced the growth rate of the mold fungus Aspergillus unguis VKM F-1754 by 70% relative to the control. The antimicrobial activity of P. frigoritolerans VKM V-3700D was insignificant. At the same time, a mixture of cultures P. frigoritolerans VKM B-3700D/B. subtilis VKM B-3701D reduced the growth rate of A. unguis VKM F-1754 by 24.5%. It has been shown that strain A1.1 is able to use nitrogen compounds for assimilation processes. It can be assumed that P. frigoritolerans VKM V-3700D belongs to the group of nitrifying or denitrifying microorganisms, which may be important in developing methods for reducing nitrogen load and eutrophication.
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spelling pubmed-105376462023-09-29 Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia Senchenkov, Vladislav Yu. Lyakhovchenko, Nikita S. Nikishin, Ilya A. Myagkov, Dmitry A. Chepurina, Anna A. Polivtseva, Valentina N. Abashina, Tatiana N. Delegan, Yanina A. Nikulicheva, Tatiana B. Nikulin, Ivan S. Bogun, Alexander G. Solomentsev, Viktor I. Solyanikova, Inna P. Microorganisms Article Bacteria, designated as A1.1 and A1.2, were isolated from poultry waste based on the ability to form ammonia on LB nutrient medium. Whole genome sequencing identified the studied strains as Peribacillus frigoritolerans VKM B-3700D (A1.1) and Bacillus subtilis VKM B-3701D (A1.2) with genome sizes of 5462638 and 4158287 bp, respectively. In the genome of B. subtilis VKM B-3701D, gene clusters of secondary metabolites of bacillin, subtilisin, bacilisin, surfactin, bacilliacin, fengycin, sactipeptide, and ratipeptide (spore killing factor) with potential antimicrobial activity were identified. Clusters of coronimine and peninodin production genes were found in P. frigoritolerans VKM B-3700D. Information on coronimine in bacteria is extremely limited. The study of the individual properties of the strains showed that the cultures are capable of biosynthesis of a number of enzymes, including amylases. The B. subtilis VKM V-3701D inhibited the growth of bacterial test cultures and reduced the growth rate of the mold fungus Aspergillus unguis VKM F-1754 by 70% relative to the control. The antimicrobial activity of P. frigoritolerans VKM V-3700D was insignificant. At the same time, a mixture of cultures P. frigoritolerans VKM B-3700D/B. subtilis VKM B-3701D reduced the growth rate of A. unguis VKM F-1754 by 24.5%. It has been shown that strain A1.1 is able to use nitrogen compounds for assimilation processes. It can be assumed that P. frigoritolerans VKM V-3700D belongs to the group of nitrifying or denitrifying microorganisms, which may be important in developing methods for reducing nitrogen load and eutrophication. MDPI 2023-09-05 /pmc/articles/PMC10537646/ /pubmed/37764079 http://dx.doi.org/10.3390/microorganisms11092235 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Senchenkov, Vladislav Yu.
Lyakhovchenko, Nikita S.
Nikishin, Ilya A.
Myagkov, Dmitry A.
Chepurina, Anna A.
Polivtseva, Valentina N.
Abashina, Tatiana N.
Delegan, Yanina A.
Nikulicheva, Tatiana B.
Nikulin, Ivan S.
Bogun, Alexander G.
Solomentsev, Viktor I.
Solyanikova, Inna P.
Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia
title Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia
title_full Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia
title_fullStr Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia
title_full_unstemmed Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia
title_short Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia
title_sort whole-genome sequencing and biotechnological potential assessment of two bacterial strains isolated from poultry farms in belgorod, russia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537646/
https://www.ncbi.nlm.nih.gov/pubmed/37764079
http://dx.doi.org/10.3390/microorganisms11092235
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