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Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites

Boswellia serrata Roxb. extract (BSE), rich in boswellic acids, is well known as a potent anti-inflammatory natural drug. However, due to its limited aqueous solubility, BSE inclusion into an appropriate carrier, capable of improving its release in the biological target, would be highly desirable. S...

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Autores principales: Cometa, Stefania, Busto, Francesco, Castellaneta, Andrea, Cochis, Andrea, Najmi, Ziba, Rizzi, Rosanna, Losito, Ilario, De Giglio, Elvira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537998/
https://www.ncbi.nlm.nih.gov/pubmed/37764225
http://dx.doi.org/10.3390/molecules28186449
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author Cometa, Stefania
Busto, Francesco
Castellaneta, Andrea
Cochis, Andrea
Najmi, Ziba
Rizzi, Rosanna
Losito, Ilario
De Giglio, Elvira
author_facet Cometa, Stefania
Busto, Francesco
Castellaneta, Andrea
Cochis, Andrea
Najmi, Ziba
Rizzi, Rosanna
Losito, Ilario
De Giglio, Elvira
author_sort Cometa, Stefania
collection PubMed
description Boswellia serrata Roxb. extract (BSE), rich in boswellic acids, is well known as a potent anti-inflammatory natural drug. However, due to its limited aqueous solubility, BSE inclusion into an appropriate carrier, capable of improving its release in the biological target, would be highly desirable. Starting with this requirement, new hybrid composites based on the inclusion of BSE in a lamellar solid layered double hydroxide (LDH), i.e., magnesium aluminum carbonate, were developed and characterized in the present work. The adopted LDH exhibited a layered crystal structure, comprising positively charged hydroxide layers and interlayers composed of carbonate anions and water molecules; thus, it was expected to embed negatively charged boswellic acids. In the present case, a calcination process was also adopted on the LDH to increase organic acid loading, based on the replacement of the original inorganic anions. An accurate investigation was carried out by TGA, PXRD, FT-IR/ATR, XPS, SEM, and LC-MS to ascertain the nature, interaction, and quantification of the active molecules of the vegetal extract loaded in the developed hybrid materials. As a result, the significant disruption of the original layered structure was observed in the LDH subjected to calcination (LDHc), and this material was able to include a higher amount of organic acids when its composite with BSE was prepared. However, in vitro tests on the composites’ bioactivity, expressed in terms of antimicrobial and anti-inflammatory activity, evidenced LDH–BSE as a better material compared to BSE and to LDHc–BSE, thus suggesting that, although the embedded organic acid amount was lower, they could be more available since they were not firmly bound to the clay. The composite was able to significantly decrease the number of viable pathogens such as Escherichia coli and Staphylococcus aureus, as well as the internalization of toxic active species into human cells imposing oxidative stress, in comparison to the BSE.
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spelling pubmed-105379982023-09-29 Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites Cometa, Stefania Busto, Francesco Castellaneta, Andrea Cochis, Andrea Najmi, Ziba Rizzi, Rosanna Losito, Ilario De Giglio, Elvira Molecules Article Boswellia serrata Roxb. extract (BSE), rich in boswellic acids, is well known as a potent anti-inflammatory natural drug. However, due to its limited aqueous solubility, BSE inclusion into an appropriate carrier, capable of improving its release in the biological target, would be highly desirable. Starting with this requirement, new hybrid composites based on the inclusion of BSE in a lamellar solid layered double hydroxide (LDH), i.e., magnesium aluminum carbonate, were developed and characterized in the present work. The adopted LDH exhibited a layered crystal structure, comprising positively charged hydroxide layers and interlayers composed of carbonate anions and water molecules; thus, it was expected to embed negatively charged boswellic acids. In the present case, a calcination process was also adopted on the LDH to increase organic acid loading, based on the replacement of the original inorganic anions. An accurate investigation was carried out by TGA, PXRD, FT-IR/ATR, XPS, SEM, and LC-MS to ascertain the nature, interaction, and quantification of the active molecules of the vegetal extract loaded in the developed hybrid materials. As a result, the significant disruption of the original layered structure was observed in the LDH subjected to calcination (LDHc), and this material was able to include a higher amount of organic acids when its composite with BSE was prepared. However, in vitro tests on the composites’ bioactivity, expressed in terms of antimicrobial and anti-inflammatory activity, evidenced LDH–BSE as a better material compared to BSE and to LDHc–BSE, thus suggesting that, although the embedded organic acid amount was lower, they could be more available since they were not firmly bound to the clay. The composite was able to significantly decrease the number of viable pathogens such as Escherichia coli and Staphylococcus aureus, as well as the internalization of toxic active species into human cells imposing oxidative stress, in comparison to the BSE. MDPI 2023-09-05 /pmc/articles/PMC10537998/ /pubmed/37764225 http://dx.doi.org/10.3390/molecules28186449 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cometa, Stefania
Busto, Francesco
Castellaneta, Andrea
Cochis, Andrea
Najmi, Ziba
Rizzi, Rosanna
Losito, Ilario
De Giglio, Elvira
Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites
title Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites
title_full Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites
title_fullStr Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites
title_full_unstemmed Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites
title_short Development, Analytical Characterization, and Bioactivity Evaluation of Boswellia serrata Extract-Layered Double Hydroxide Hybrid Composites
title_sort development, analytical characterization, and bioactivity evaluation of boswellia serrata extract-layered double hydroxide hybrid composites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537998/
https://www.ncbi.nlm.nih.gov/pubmed/37764225
http://dx.doi.org/10.3390/molecules28186449
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