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Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes
Chitosan is obtained from the deacetylation of chitin, and it is known to possess antimicrobial activity. It has attracted attention as it may be used for treating infections caused by different types of microorganisms due to its broad spectrum. Its application in the form of micro- or nanoparticles...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538158/ https://www.ncbi.nlm.nih.gov/pubmed/37765613 http://dx.doi.org/10.3390/polym15183759 |
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author | Pereira, Sara Costa-Ribeiro, Ana Teixeira, Pilar Rodríguez-Lorenzo, Laura Prado, Marta Cerqueira, Miguel A. Garrido-Maestu, Alejandro |
author_facet | Pereira, Sara Costa-Ribeiro, Ana Teixeira, Pilar Rodríguez-Lorenzo, Laura Prado, Marta Cerqueira, Miguel A. Garrido-Maestu, Alejandro |
author_sort | Pereira, Sara |
collection | PubMed |
description | Chitosan is obtained from the deacetylation of chitin, and it is known to possess antimicrobial activity. It has attracted attention as it may be used for treating infections caused by different types of microorganisms due to its broad spectrum. Its application in the form of micro- or nanoparticles (CM/CN) has expanded its usage, as in this form, it retains its activity, and remain stable in aqueous solutions. However, inconsistencies in the results reported by different authors have been identified. In this communication, the antimicrobial activity of CN produced from different starting materials was tested against Listeria monocytogenes. It was observed that, even though all the starting materials were reported to have a molecular weight (MW) below 200 kDa and degree of deacetylation (DD) > 75%, the size of the CNs were significantly different (263 nm vs. 607 nm). Furthermore, these differences in sizes exerted a direct effect on the antimicrobial properties of the particles, as when testing the ones with the smallest size, i.e., 263 nm, a lower Minimum Inhibitory Concentration (MIC) was achieved, i.e., 0.04 mg/mL. Even though the largest particles, i.e., 607 nm, in individual experiments were able to achieve an MIC of 0.03 mg/mL, the results with CN presented great variation among replicates and up to 0.2 mg/mL were needed in other replicates. The starting material has a critical impact on the properties of the CN, and it must be carefully characterized and selected for the intended application, and MW and DD solely do not fully account for these properties. |
format | Online Article Text |
id | pubmed-10538158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105381582023-09-29 Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes Pereira, Sara Costa-Ribeiro, Ana Teixeira, Pilar Rodríguez-Lorenzo, Laura Prado, Marta Cerqueira, Miguel A. Garrido-Maestu, Alejandro Polymers (Basel) Communication Chitosan is obtained from the deacetylation of chitin, and it is known to possess antimicrobial activity. It has attracted attention as it may be used for treating infections caused by different types of microorganisms due to its broad spectrum. Its application in the form of micro- or nanoparticles (CM/CN) has expanded its usage, as in this form, it retains its activity, and remain stable in aqueous solutions. However, inconsistencies in the results reported by different authors have been identified. In this communication, the antimicrobial activity of CN produced from different starting materials was tested against Listeria monocytogenes. It was observed that, even though all the starting materials were reported to have a molecular weight (MW) below 200 kDa and degree of deacetylation (DD) > 75%, the size of the CNs were significantly different (263 nm vs. 607 nm). Furthermore, these differences in sizes exerted a direct effect on the antimicrobial properties of the particles, as when testing the ones with the smallest size, i.e., 263 nm, a lower Minimum Inhibitory Concentration (MIC) was achieved, i.e., 0.04 mg/mL. Even though the largest particles, i.e., 607 nm, in individual experiments were able to achieve an MIC of 0.03 mg/mL, the results with CN presented great variation among replicates and up to 0.2 mg/mL were needed in other replicates. The starting material has a critical impact on the properties of the CN, and it must be carefully characterized and selected for the intended application, and MW and DD solely do not fully account for these properties. MDPI 2023-09-14 /pmc/articles/PMC10538158/ /pubmed/37765613 http://dx.doi.org/10.3390/polym15183759 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Pereira, Sara Costa-Ribeiro, Ana Teixeira, Pilar Rodríguez-Lorenzo, Laura Prado, Marta Cerqueira, Miguel A. Garrido-Maestu, Alejandro Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes |
title | Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes |
title_full | Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes |
title_fullStr | Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes |
title_full_unstemmed | Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes |
title_short | Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes |
title_sort | evaluation of the antimicrobial activity of chitosan nanoparticles against listeria monocytogenes |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538158/ https://www.ncbi.nlm.nih.gov/pubmed/37765613 http://dx.doi.org/10.3390/polym15183759 |
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