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Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury

Ischemia–reperfusion injury (IRI) is a common phenomenon that develops both from natural causes and during major operations. Many intracellular processes mediated by calcium ions are involved in the development of IRI. Currently, chemical calcium channel blockers are used but they have a number of l...

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Autores principales: Iurova, Elena, Rastorgueva, Eugenia, Beloborodov, Evgenii, Pogodina, Evgeniya, Fomin, Aleksandr, Sugak, Dmitrii, Viktorov, Denis, Tumozov, Ivan, Saenko, Yury
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538190/
https://www.ncbi.nlm.nih.gov/pubmed/37765122
http://dx.doi.org/10.3390/ph16091314
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author Iurova, Elena
Rastorgueva, Eugenia
Beloborodov, Evgenii
Pogodina, Evgeniya
Fomin, Aleksandr
Sugak, Dmitrii
Viktorov, Denis
Tumozov, Ivan
Saenko, Yury
author_facet Iurova, Elena
Rastorgueva, Eugenia
Beloborodov, Evgenii
Pogodina, Evgeniya
Fomin, Aleksandr
Sugak, Dmitrii
Viktorov, Denis
Tumozov, Ivan
Saenko, Yury
author_sort Iurova, Elena
collection PubMed
description Ischemia–reperfusion injury (IRI) is a common phenomenon that develops both from natural causes and during major operations. Many intracellular processes mediated by calcium ions are involved in the development of IRI. Currently, chemical calcium channel blockers are used but they have a number of limitations. In this article, we study the effect of the omega-hexatoxin-Hv1a peptide toxin, an alternative to chemical calcium channel blockers, on the mechanisms of IRI development in epithelial cell culture. The toxin was produced using solid phase peptide synthesis. IRI was caused by deprivation of glucose, serum and oxygen. The data obtained demonstrate that the omega-hexatoxin-Hv1a toxin in nanomolar concentrations is able to prevent the development of apoptosis and necrosis in epithelial cells by reducing the concentration of calcium, sodium and potassium ions, as well as by delaying rapid normalization of the pH level, affecting the mitochondrial potential and oxidative stress. This toxin can be used as an alternative to chemical calcium channel blockers for preventing tissue and organ IRI due to its low-dose requirement and high bioavailability.
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spelling pubmed-105381902023-09-29 Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury Iurova, Elena Rastorgueva, Eugenia Beloborodov, Evgenii Pogodina, Evgeniya Fomin, Aleksandr Sugak, Dmitrii Viktorov, Denis Tumozov, Ivan Saenko, Yury Pharmaceuticals (Basel) Article Ischemia–reperfusion injury (IRI) is a common phenomenon that develops both from natural causes and during major operations. Many intracellular processes mediated by calcium ions are involved in the development of IRI. Currently, chemical calcium channel blockers are used but they have a number of limitations. In this article, we study the effect of the omega-hexatoxin-Hv1a peptide toxin, an alternative to chemical calcium channel blockers, on the mechanisms of IRI development in epithelial cell culture. The toxin was produced using solid phase peptide synthesis. IRI was caused by deprivation of glucose, serum and oxygen. The data obtained demonstrate that the omega-hexatoxin-Hv1a toxin in nanomolar concentrations is able to prevent the development of apoptosis and necrosis in epithelial cells by reducing the concentration of calcium, sodium and potassium ions, as well as by delaying rapid normalization of the pH level, affecting the mitochondrial potential and oxidative stress. This toxin can be used as an alternative to chemical calcium channel blockers for preventing tissue and organ IRI due to its low-dose requirement and high bioavailability. MDPI 2023-09-18 /pmc/articles/PMC10538190/ /pubmed/37765122 http://dx.doi.org/10.3390/ph16091314 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iurova, Elena
Rastorgueva, Eugenia
Beloborodov, Evgenii
Pogodina, Evgeniya
Fomin, Aleksandr
Sugak, Dmitrii
Viktorov, Denis
Tumozov, Ivan
Saenko, Yury
Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury
title Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury
title_full Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury
title_fullStr Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury
title_full_unstemmed Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury
title_short Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury
title_sort protective effect of peptide calcium channel blocker omega-hexatoxin-hv1a on epithelial cell during ischemia–reperfusion injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538190/
https://www.ncbi.nlm.nih.gov/pubmed/37765122
http://dx.doi.org/10.3390/ph16091314
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