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Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
BACKGROUND AND OBJECTIVE: Periodontitis is a multifactorial disease initiated by periodontal pathogens and progresses further in destruction of periodontium. Hence, the objective of this study was to test the efficacy of Ocimum basilicum seeds extract on periodontal pathogens. MATERIALS AND METHODS:...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538503/ https://www.ncbi.nlm.nih.gov/pubmed/37781327 http://dx.doi.org/10.4103/jisp.jisp_406_22 |
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author | Khot, Atrey J. Pai Ankola, Anil V. Dodamani, Suneel Sankeshwari, Roopali M. Kumar, Ram Surath Santhosh, Varkey Nadakkavukaran |
author_facet | Khot, Atrey J. Pai Ankola, Anil V. Dodamani, Suneel Sankeshwari, Roopali M. Kumar, Ram Surath Santhosh, Varkey Nadakkavukaran |
author_sort | Khot, Atrey J. Pai |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: Periodontitis is a multifactorial disease initiated by periodontal pathogens and progresses further in destruction of periodontium. Hence, the objective of this study was to test the efficacy of Ocimum basilicum seeds extract on periodontal pathogens. MATERIALS AND METHODS: O. basilicum seeds were authenticated from a recognized taxonomist. They were coarsely powdered; ethanol-based extract preparation was done by the Soxhlet method and aqueous-based extract by hot infusion procedure. Extracts so obtained were assessed for minimum inhibitory concentration, minimum bactericidal concentration, zone of inhibition, and time-kill assay of O. basilicum seeds extract on periodontal pathogens, and comparatively evaluated the effectiveness against 0.12% chlorhexidine (CHX) gluconate in triplicates. Kruskal-Wallis Test was employed wherein the statistical significance was set at P ≤ 0.05. RESULTS: The concentration of O. basilicum ethanolic extract against periodontal pathogens was determined to be 10 mg/ml, whereas 4.7 mg/ml of aqueous extract was proven effective against periodontal pathogens. Similarly, aqueous extract of O. basilicum developed a wider zone against periodontal pathogens compared to ethanol-based O. basilicum extract. Statistically significant difference found in the effectiveness between both extract and CHX. CONCLUSION: The antibacterial activity was evident in both the extracts of O. basilicum against anaerobic periodontal pathogens. However, it was more pronounced in aqueous extract, but lower compared to CHX. |
format | Online Article Text |
id | pubmed-10538503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-105385032023-09-29 Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study Khot, Atrey J. Pai Ankola, Anil V. Dodamani, Suneel Sankeshwari, Roopali M. Kumar, Ram Surath Santhosh, Varkey Nadakkavukaran J Indian Soc Periodontol Original Article BACKGROUND AND OBJECTIVE: Periodontitis is a multifactorial disease initiated by periodontal pathogens and progresses further in destruction of periodontium. Hence, the objective of this study was to test the efficacy of Ocimum basilicum seeds extract on periodontal pathogens. MATERIALS AND METHODS: O. basilicum seeds were authenticated from a recognized taxonomist. They were coarsely powdered; ethanol-based extract preparation was done by the Soxhlet method and aqueous-based extract by hot infusion procedure. Extracts so obtained were assessed for minimum inhibitory concentration, minimum bactericidal concentration, zone of inhibition, and time-kill assay of O. basilicum seeds extract on periodontal pathogens, and comparatively evaluated the effectiveness against 0.12% chlorhexidine (CHX) gluconate in triplicates. Kruskal-Wallis Test was employed wherein the statistical significance was set at P ≤ 0.05. RESULTS: The concentration of O. basilicum ethanolic extract against periodontal pathogens was determined to be 10 mg/ml, whereas 4.7 mg/ml of aqueous extract was proven effective against periodontal pathogens. Similarly, aqueous extract of O. basilicum developed a wider zone against periodontal pathogens compared to ethanol-based O. basilicum extract. Statistically significant difference found in the effectiveness between both extract and CHX. CONCLUSION: The antibacterial activity was evident in both the extracts of O. basilicum against anaerobic periodontal pathogens. However, it was more pronounced in aqueous extract, but lower compared to CHX. Wolters Kluwer - Medknow 2023 2023-09-01 /pmc/articles/PMC10538503/ /pubmed/37781327 http://dx.doi.org/10.4103/jisp.jisp_406_22 Text en Copyright: © 2023 Indian Society of Periodontology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Khot, Atrey J. Pai Ankola, Anil V. Dodamani, Suneel Sankeshwari, Roopali M. Kumar, Ram Surath Santhosh, Varkey Nadakkavukaran Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study |
title | Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study |
title_full | Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study |
title_fullStr | Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study |
title_full_unstemmed | Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study |
title_short | Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study |
title_sort | assessment of potential antimicrobial activity of ocimum basilicum extract and chlorhexidine against socransky’s complex pathogens of oral cavity: an in vitro study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538503/ https://www.ncbi.nlm.nih.gov/pubmed/37781327 http://dx.doi.org/10.4103/jisp.jisp_406_22 |
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