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Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study

BACKGROUND AND OBJECTIVE: Periodontitis is a multifactorial disease initiated by periodontal pathogens and progresses further in destruction of periodontium. Hence, the objective of this study was to test the efficacy of Ocimum basilicum seeds extract on periodontal pathogens. MATERIALS AND METHODS:...

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Autores principales: Khot, Atrey J. Pai, Ankola, Anil V., Dodamani, Suneel, Sankeshwari, Roopali M., Kumar, Ram Surath, Santhosh, Varkey Nadakkavukaran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538503/
https://www.ncbi.nlm.nih.gov/pubmed/37781327
http://dx.doi.org/10.4103/jisp.jisp_406_22
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author Khot, Atrey J. Pai
Ankola, Anil V.
Dodamani, Suneel
Sankeshwari, Roopali M.
Kumar, Ram Surath
Santhosh, Varkey Nadakkavukaran
author_facet Khot, Atrey J. Pai
Ankola, Anil V.
Dodamani, Suneel
Sankeshwari, Roopali M.
Kumar, Ram Surath
Santhosh, Varkey Nadakkavukaran
author_sort Khot, Atrey J. Pai
collection PubMed
description BACKGROUND AND OBJECTIVE: Periodontitis is a multifactorial disease initiated by periodontal pathogens and progresses further in destruction of periodontium. Hence, the objective of this study was to test the efficacy of Ocimum basilicum seeds extract on periodontal pathogens. MATERIALS AND METHODS: O. basilicum seeds were authenticated from a recognized taxonomist. They were coarsely powdered; ethanol-based extract preparation was done by the Soxhlet method and aqueous-based extract by hot infusion procedure. Extracts so obtained were assessed for minimum inhibitory concentration, minimum bactericidal concentration, zone of inhibition, and time-kill assay of O. basilicum seeds extract on periodontal pathogens, and comparatively evaluated the effectiveness against 0.12% chlorhexidine (CHX) gluconate in triplicates. Kruskal-Wallis Test was employed wherein the statistical significance was set at P ≤ 0.05. RESULTS: The concentration of O. basilicum ethanolic extract against periodontal pathogens was determined to be 10 mg/ml, whereas 4.7 mg/ml of aqueous extract was proven effective against periodontal pathogens. Similarly, aqueous extract of O. basilicum developed a wider zone against periodontal pathogens compared to ethanol-based O. basilicum extract. Statistically significant difference found in the effectiveness between both extract and CHX. CONCLUSION: The antibacterial activity was evident in both the extracts of O. basilicum against anaerobic periodontal pathogens. However, it was more pronounced in aqueous extract, but lower compared to CHX.
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spelling pubmed-105385032023-09-29 Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study Khot, Atrey J. Pai Ankola, Anil V. Dodamani, Suneel Sankeshwari, Roopali M. Kumar, Ram Surath Santhosh, Varkey Nadakkavukaran J Indian Soc Periodontol Original Article BACKGROUND AND OBJECTIVE: Periodontitis is a multifactorial disease initiated by periodontal pathogens and progresses further in destruction of periodontium. Hence, the objective of this study was to test the efficacy of Ocimum basilicum seeds extract on periodontal pathogens. MATERIALS AND METHODS: O. basilicum seeds were authenticated from a recognized taxonomist. They were coarsely powdered; ethanol-based extract preparation was done by the Soxhlet method and aqueous-based extract by hot infusion procedure. Extracts so obtained were assessed for minimum inhibitory concentration, minimum bactericidal concentration, zone of inhibition, and time-kill assay of O. basilicum seeds extract on periodontal pathogens, and comparatively evaluated the effectiveness against 0.12% chlorhexidine (CHX) gluconate in triplicates. Kruskal-Wallis Test was employed wherein the statistical significance was set at P ≤ 0.05. RESULTS: The concentration of O. basilicum ethanolic extract against periodontal pathogens was determined to be 10 mg/ml, whereas 4.7 mg/ml of aqueous extract was proven effective against periodontal pathogens. Similarly, aqueous extract of O. basilicum developed a wider zone against periodontal pathogens compared to ethanol-based O. basilicum extract. Statistically significant difference found in the effectiveness between both extract and CHX. CONCLUSION: The antibacterial activity was evident in both the extracts of O. basilicum against anaerobic periodontal pathogens. However, it was more pronounced in aqueous extract, but lower compared to CHX. Wolters Kluwer - Medknow 2023 2023-09-01 /pmc/articles/PMC10538503/ /pubmed/37781327 http://dx.doi.org/10.4103/jisp.jisp_406_22 Text en Copyright: © 2023 Indian Society of Periodontology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Khot, Atrey J. Pai
Ankola, Anil V.
Dodamani, Suneel
Sankeshwari, Roopali M.
Kumar, Ram Surath
Santhosh, Varkey Nadakkavukaran
Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
title Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
title_full Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
title_fullStr Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
title_full_unstemmed Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
title_short Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: An in vitro study
title_sort assessment of potential antimicrobial activity of ocimum basilicum extract and chlorhexidine against socransky’s complex pathogens of oral cavity: an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538503/
https://www.ncbi.nlm.nih.gov/pubmed/37781327
http://dx.doi.org/10.4103/jisp.jisp_406_22
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