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Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue

Studies have documented the high occurrence of several tumors, including female breast cancer, in populations occupationally exposed to pesticides worldwide. It is believed that in addition to direct DNA damage, other molecular alterations that indicate genomic instability are associated, such as ep...

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Autores principales: da Silva, Ruan Gabriel Soares, Ferreira, Mariane Okamoto, Komori, Isabella Mitsu Suo, Oliveira, Henrique Rodrigues Menezes, Machado, Murilo Galvani, Orrutea, Julia Fernandes Gois, Alves, Fernanda Mara, dos Santos Jaques, Hellen, da Silva, Janaína Carla, de Souza, Janoário Athanazio, Rech, Daniel, Panis, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538633/
https://www.ncbi.nlm.nih.gov/pubmed/37780419
http://dx.doi.org/10.3389/fpubh.2023.1229422
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author da Silva, Ruan Gabriel Soares
Ferreira, Mariane Okamoto
Komori, Isabella Mitsu Suo
Oliveira, Henrique Rodrigues Menezes
Machado, Murilo Galvani
Orrutea, Julia Fernandes Gois
Alves, Fernanda Mara
dos Santos Jaques, Hellen
da Silva, Janaína Carla
de Souza, Janoário Athanazio
Rech, Daniel
Panis, Carolina
author_facet da Silva, Ruan Gabriel Soares
Ferreira, Mariane Okamoto
Komori, Isabella Mitsu Suo
Oliveira, Henrique Rodrigues Menezes
Machado, Murilo Galvani
Orrutea, Julia Fernandes Gois
Alves, Fernanda Mara
dos Santos Jaques, Hellen
da Silva, Janaína Carla
de Souza, Janoário Athanazio
Rech, Daniel
Panis, Carolina
author_sort da Silva, Ruan Gabriel Soares
collection PubMed
description Studies have documented the high occurrence of several tumors, including female breast cancer, in populations occupationally exposed to pesticides worldwide. It is believed that in addition to direct DNA damage, other molecular alterations that indicate genomic instability are associated, such as epigenetic modifications and the production of inflammation mediators. The present study characterized the profile of inflammatory changes in the breast tissue of women without cancer occupationally exposed to pesticides. In samples of normal breast tissue collected during biopsy and evaluated as negative for cancer by a pathologist, oxidative stress levels were assessed as inflammatory markers through measurements of lipoperoxides and total antioxidant capacity of the sample (TRAP) by high-sensitivity chemiluminescence, as well as levels of nitric oxide (NOx) metabolites. The levels of inflammation-modulating transcription factors PPAR-γ (peroxisome proliferator-activated receptor gamma) and NF-κB (nuclear factor kappa B) were also quantified, in addition to the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin 12 (IL-12). The levels of lipoperoxides, TRAP, and NOx were significantly lower in the exposed group. On the other hand, PPAR-γ levels were increased in the breast tissue of exposed women, with no variation in NF-κB. There was also a rise of TNF-α in exposed women samples without significant variations in IL-12 levels. These findings suggest an inflammatory signature of the breast tissue associated with pesticide exposure, which may trigger mechanisms related to mutations and breast carcinogenesis.
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spelling pubmed-105386332023-09-29 Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue da Silva, Ruan Gabriel Soares Ferreira, Mariane Okamoto Komori, Isabella Mitsu Suo Oliveira, Henrique Rodrigues Menezes Machado, Murilo Galvani Orrutea, Julia Fernandes Gois Alves, Fernanda Mara dos Santos Jaques, Hellen da Silva, Janaína Carla de Souza, Janoário Athanazio Rech, Daniel Panis, Carolina Front Public Health Public Health Studies have documented the high occurrence of several tumors, including female breast cancer, in populations occupationally exposed to pesticides worldwide. It is believed that in addition to direct DNA damage, other molecular alterations that indicate genomic instability are associated, such as epigenetic modifications and the production of inflammation mediators. The present study characterized the profile of inflammatory changes in the breast tissue of women without cancer occupationally exposed to pesticides. In samples of normal breast tissue collected during biopsy and evaluated as negative for cancer by a pathologist, oxidative stress levels were assessed as inflammatory markers through measurements of lipoperoxides and total antioxidant capacity of the sample (TRAP) by high-sensitivity chemiluminescence, as well as levels of nitric oxide (NOx) metabolites. The levels of inflammation-modulating transcription factors PPAR-γ (peroxisome proliferator-activated receptor gamma) and NF-κB (nuclear factor kappa B) were also quantified, in addition to the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin 12 (IL-12). The levels of lipoperoxides, TRAP, and NOx were significantly lower in the exposed group. On the other hand, PPAR-γ levels were increased in the breast tissue of exposed women, with no variation in NF-κB. There was also a rise of TNF-α in exposed women samples without significant variations in IL-12 levels. These findings suggest an inflammatory signature of the breast tissue associated with pesticide exposure, which may trigger mechanisms related to mutations and breast carcinogenesis. Frontiers Media S.A. 2023-09-14 /pmc/articles/PMC10538633/ /pubmed/37780419 http://dx.doi.org/10.3389/fpubh.2023.1229422 Text en Copyright © 2023 da Silva, Ferreira, Komori, Oliveira, Machado, Orrutea, Alves, dos Santos Jaques, da Silva, de Souza, Rech and Panis. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
da Silva, Ruan Gabriel Soares
Ferreira, Mariane Okamoto
Komori, Isabella Mitsu Suo
Oliveira, Henrique Rodrigues Menezes
Machado, Murilo Galvani
Orrutea, Julia Fernandes Gois
Alves, Fernanda Mara
dos Santos Jaques, Hellen
da Silva, Janaína Carla
de Souza, Janoário Athanazio
Rech, Daniel
Panis, Carolina
Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
title Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
title_full Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
title_fullStr Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
title_full_unstemmed Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
title_short Brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
title_sort brief research report pesticide occupational exposure leads to significant inflammatory changes in normal mammary breast tissue
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538633/
https://www.ncbi.nlm.nih.gov/pubmed/37780419
http://dx.doi.org/10.3389/fpubh.2023.1229422
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