Cargando…

Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris

PURPOSE: This study aimed to investigate the genetic diversity of Wolbachia in field-caught bed bug species in Paris areas. METHODS: The bed bug specimens were captured from various infested localities in Paris and surrounding cities. They belonged to diverse life stages, including egg, nymph, and a...

Descripción completa

Detalles Bibliográficos
Autores principales: Chebbah, Dahlia, Hamarsheh, Omar, Sereno, Denis, Elissa, Nohal, Brun, Sophie, Jan, Julie, Izri, Arezki, Akhoundi, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538740/
https://www.ncbi.nlm.nih.gov/pubmed/37768955
http://dx.doi.org/10.1371/journal.pone.0292229
_version_ 1785113365268398080
author Chebbah, Dahlia
Hamarsheh, Omar
Sereno, Denis
Elissa, Nohal
Brun, Sophie
Jan, Julie
Izri, Arezki
Akhoundi, Mohammad
author_facet Chebbah, Dahlia
Hamarsheh, Omar
Sereno, Denis
Elissa, Nohal
Brun, Sophie
Jan, Julie
Izri, Arezki
Akhoundi, Mohammad
author_sort Chebbah, Dahlia
collection PubMed
description PURPOSE: This study aimed to investigate the genetic diversity of Wolbachia in field-caught bed bug species in Paris areas. METHODS: The bed bug specimens were captured from various infested localities in Paris and surrounding cities. They belonged to diverse life stages, including egg, nymph, and adult. They were then identified using morphological and molecular approaches. Furthermore, Wolbachia was detected, and its genetic diversity was investigated by conventional PCR of 16S-rRNA and Wolbachia surface protein (wsp) genes. RESULTS: A total of 256 bed bug specimens belonging to various life stages [adult (183 specimens), nymph (48), and egg (25)] were captured from seven private apartments, five social apartments, three houses, two immigrant residences, and one retirement home situated in 10 districts of Paris and 8 surrounding cities. They were identified as Cimex lectularius (237 specimens) and C. hemipterus (19) using morphological and molecular approaches. The presence and diversity of Wolbachia were ascertained by targeting 16S-rRNA and wsp genes. Based on molecular analysis, 182 and 148 out of 256 processed specimens were positive by amplifying 16S-rRNA and wsp fragments, respectively. The inferred phylogenetic analysis with 16S-rRNA and wsp sequences displayed monophyletic Wolbachia strains clustering each one in three populations. The median-joining network, including the Wolbachia 16S-rRNA and wsp sequences of C. lectularius and C. hemipterous specimens, indicated a significant genetic differentiation among these populations in Paris areas which was consent with Neighbor-Joining analyses. A phylogenetic analysis of our heterogenic Wolbachia sequences with those reported from other arthropod species confirmed their belonging to supergroup F. Moreover, no difference between Wolbachia sequences from eggs, nymphs, and adults belonging to the same clade and between Wolbachia sequences of C. lectularius and C. hemipterus were observed after sequence alignment. Furthermore, no significant correlation was found between multiple geographical locations (or accomodation type) where bed bugs were collected and the genetic diversity of Wolbachia. CONCLUSIONS: We highlight a significant heterogeneity within Wolbachia symbionts detected in C. lectularius and C. hemipterus. No correlation between Wolbachia species and bed bug species (C. lectularius versus C. hemipterus), physiological stages (egg, nymph, and adult), and sampling location was recorded in this study.
format Online
Article
Text
id pubmed-10538740
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-105387402023-09-29 Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris Chebbah, Dahlia Hamarsheh, Omar Sereno, Denis Elissa, Nohal Brun, Sophie Jan, Julie Izri, Arezki Akhoundi, Mohammad PLoS One Research Article PURPOSE: This study aimed to investigate the genetic diversity of Wolbachia in field-caught bed bug species in Paris areas. METHODS: The bed bug specimens were captured from various infested localities in Paris and surrounding cities. They belonged to diverse life stages, including egg, nymph, and adult. They were then identified using morphological and molecular approaches. Furthermore, Wolbachia was detected, and its genetic diversity was investigated by conventional PCR of 16S-rRNA and Wolbachia surface protein (wsp) genes. RESULTS: A total of 256 bed bug specimens belonging to various life stages [adult (183 specimens), nymph (48), and egg (25)] were captured from seven private apartments, five social apartments, three houses, two immigrant residences, and one retirement home situated in 10 districts of Paris and 8 surrounding cities. They were identified as Cimex lectularius (237 specimens) and C. hemipterus (19) using morphological and molecular approaches. The presence and diversity of Wolbachia were ascertained by targeting 16S-rRNA and wsp genes. Based on molecular analysis, 182 and 148 out of 256 processed specimens were positive by amplifying 16S-rRNA and wsp fragments, respectively. The inferred phylogenetic analysis with 16S-rRNA and wsp sequences displayed monophyletic Wolbachia strains clustering each one in three populations. The median-joining network, including the Wolbachia 16S-rRNA and wsp sequences of C. lectularius and C. hemipterous specimens, indicated a significant genetic differentiation among these populations in Paris areas which was consent with Neighbor-Joining analyses. A phylogenetic analysis of our heterogenic Wolbachia sequences with those reported from other arthropod species confirmed their belonging to supergroup F. Moreover, no difference between Wolbachia sequences from eggs, nymphs, and adults belonging to the same clade and between Wolbachia sequences of C. lectularius and C. hemipterus were observed after sequence alignment. Furthermore, no significant correlation was found between multiple geographical locations (or accomodation type) where bed bugs were collected and the genetic diversity of Wolbachia. CONCLUSIONS: We highlight a significant heterogeneity within Wolbachia symbionts detected in C. lectularius and C. hemipterus. No correlation between Wolbachia species and bed bug species (C. lectularius versus C. hemipterus), physiological stages (egg, nymph, and adult), and sampling location was recorded in this study. Public Library of Science 2023-09-28 /pmc/articles/PMC10538740/ /pubmed/37768955 http://dx.doi.org/10.1371/journal.pone.0292229 Text en © 2023 Chebbah et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chebbah, Dahlia
Hamarsheh, Omar
Sereno, Denis
Elissa, Nohal
Brun, Sophie
Jan, Julie
Izri, Arezki
Akhoundi, Mohammad
Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris
title Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris
title_full Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris
title_fullStr Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris
title_full_unstemmed Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris
title_short Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris
title_sort molecular characterization and genetic diversity of wolbachia endosymbionts in bed bugs (hemiptera; cimicidae) collected in paris
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538740/
https://www.ncbi.nlm.nih.gov/pubmed/37768955
http://dx.doi.org/10.1371/journal.pone.0292229
work_keys_str_mv AT chebbahdahlia molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT hamarshehomar molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT serenodenis molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT elissanohal molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT brunsophie molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT janjulie molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT izriarezki molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis
AT akhoundimohammad molecularcharacterizationandgeneticdiversityofwolbachiaendosymbiontsinbedbugshemipteracimicidaecollectedinparis