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Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities

Water quality parameters influence the abundance of pathogenic bacteria. The genera Aeromonas, Arcobacter, Klebsiella, and Mycobacterium are among the representative pathogenic bacteria identified in wastewater. However, information on the correlations between water quality and the abundance of thes...

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Autores principales: Aoki, Masataka, Takemura, Yasuyuki, Kawakami, Shuji, Yoochatchaval, Wilasinee, Tran P., Thao, Tomioka, Noriko, Ebie, Yoshitaka, Syutsubo, Kazuaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538766/
https://www.ncbi.nlm.nih.gov/pubmed/37768925
http://dx.doi.org/10.1371/journal.pone.0291742
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author Aoki, Masataka
Takemura, Yasuyuki
Kawakami, Shuji
Yoochatchaval, Wilasinee
Tran P., Thao
Tomioka, Noriko
Ebie, Yoshitaka
Syutsubo, Kazuaki
author_facet Aoki, Masataka
Takemura, Yasuyuki
Kawakami, Shuji
Yoochatchaval, Wilasinee
Tran P., Thao
Tomioka, Noriko
Ebie, Yoshitaka
Syutsubo, Kazuaki
author_sort Aoki, Masataka
collection PubMed
description Water quality parameters influence the abundance of pathogenic bacteria. The genera Aeromonas, Arcobacter, Klebsiella, and Mycobacterium are among the representative pathogenic bacteria identified in wastewater. However, information on the correlations between water quality and the abundance of these bacteria, as well as their reduction rate in existing wastewater treatment facilities (WTFs), is lacking. Hence, this study aimed to determine the abundance and reduction rates of these bacterial groups in WTFs. Sixty-eight samples (34 influent and 34 non-disinfected, treated, effluent samples) were collected from nine WTFs in Japan and Thailand. 16S rRNA gene amplicon sequencing analysis revealed the presence of Aeromonas, Arcobacter, and Mycobacterium in all influent wastewater and treated effluent samples. Quantitative real-time polymerase chain reaction (qPCR) was used to quantify the abundance of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex (KpSC), and Mycobacterium. The geometric mean abundances of Aeromonas, Arcobacter, KpSC, and Mycobacterium in the influent wastewater were 1.2 × 10(4)–2.4 × 10(5), 1.0 × 10(5)–4.5 × 10(6), 3.6 × 10(2)–4.3 × 10(4), and 6.9 × 10(3)–5.5 × 10(4) cells mL(−1), respectively, and their average log reduction values were 0.77–2.57, 1.00–3.06, 1.35–3.11, and −0.67–1.57, respectively. Spearman’s rank correlation coefficients indicated significant positive or negative correlations between the abundances of the potentially pathogenic bacterial groups and Escherichia coli as well as water quality parameters, namely, chemical/biochemical oxygen demand, total nitrogen, nitrate-nitrogen, nitrite-nitrogen, ammonium-nitrogen, suspended solids, volatile suspended solids, and oxidation-reduction potential. This study provides valuable information on the development and appropriate management of WTFs to produce safe, hygienic water.
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spelling pubmed-105387662023-09-29 Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities Aoki, Masataka Takemura, Yasuyuki Kawakami, Shuji Yoochatchaval, Wilasinee Tran P., Thao Tomioka, Noriko Ebie, Yoshitaka Syutsubo, Kazuaki PLoS One Research Article Water quality parameters influence the abundance of pathogenic bacteria. The genera Aeromonas, Arcobacter, Klebsiella, and Mycobacterium are among the representative pathogenic bacteria identified in wastewater. However, information on the correlations between water quality and the abundance of these bacteria, as well as their reduction rate in existing wastewater treatment facilities (WTFs), is lacking. Hence, this study aimed to determine the abundance and reduction rates of these bacterial groups in WTFs. Sixty-eight samples (34 influent and 34 non-disinfected, treated, effluent samples) were collected from nine WTFs in Japan and Thailand. 16S rRNA gene amplicon sequencing analysis revealed the presence of Aeromonas, Arcobacter, and Mycobacterium in all influent wastewater and treated effluent samples. Quantitative real-time polymerase chain reaction (qPCR) was used to quantify the abundance of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex (KpSC), and Mycobacterium. The geometric mean abundances of Aeromonas, Arcobacter, KpSC, and Mycobacterium in the influent wastewater were 1.2 × 10(4)–2.4 × 10(5), 1.0 × 10(5)–4.5 × 10(6), 3.6 × 10(2)–4.3 × 10(4), and 6.9 × 10(3)–5.5 × 10(4) cells mL(−1), respectively, and their average log reduction values were 0.77–2.57, 1.00–3.06, 1.35–3.11, and −0.67–1.57, respectively. Spearman’s rank correlation coefficients indicated significant positive or negative correlations between the abundances of the potentially pathogenic bacterial groups and Escherichia coli as well as water quality parameters, namely, chemical/biochemical oxygen demand, total nitrogen, nitrate-nitrogen, nitrite-nitrogen, ammonium-nitrogen, suspended solids, volatile suspended solids, and oxidation-reduction potential. This study provides valuable information on the development and appropriate management of WTFs to produce safe, hygienic water. Public Library of Science 2023-09-28 /pmc/articles/PMC10538766/ /pubmed/37768925 http://dx.doi.org/10.1371/journal.pone.0291742 Text en © 2023 Aoki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Aoki, Masataka
Takemura, Yasuyuki
Kawakami, Shuji
Yoochatchaval, Wilasinee
Tran P., Thao
Tomioka, Noriko
Ebie, Yoshitaka
Syutsubo, Kazuaki
Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities
title Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities
title_full Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities
title_fullStr Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities
title_full_unstemmed Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities
title_short Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities
title_sort quantitative detection and reduction of potentially pathogenic bacterial groups of aeromonas, arcobacter, klebsiella pneumoniae species complex, and mycobacterium in wastewater treatment facilities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538766/
https://www.ncbi.nlm.nih.gov/pubmed/37768925
http://dx.doi.org/10.1371/journal.pone.0291742
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