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Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity

The benefits of exercise involve skeletal muscle redox state alterations of nicotinamide adenine dinucleotide (NAD) and flavin adenine dinucleotide (FAD). We determined the fiber-specific effects of acute exercise on the skeletal muscle redox state in healthy adults. Muscle biopsies were obtained fr...

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Autores principales: Shadiow, James, Miranda, Edwin R., Perkins, Ryan K., Mazo, Corey E., Lin, Zhen, Lewis, Kendell N., Mey, Jacob T., Solomon, Thomas P. J., Haus, Jacob M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538995/
https://www.ncbi.nlm.nih.gov/pubmed/37471216
http://dx.doi.org/10.1152/japplphysiol.00662.2022
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author Shadiow, James
Miranda, Edwin R.
Perkins, Ryan K.
Mazo, Corey E.
Lin, Zhen
Lewis, Kendell N.
Mey, Jacob T.
Solomon, Thomas P. J.
Haus, Jacob M.
author_facet Shadiow, James
Miranda, Edwin R.
Perkins, Ryan K.
Mazo, Corey E.
Lin, Zhen
Lewis, Kendell N.
Mey, Jacob T.
Solomon, Thomas P. J.
Haus, Jacob M.
author_sort Shadiow, James
collection PubMed
description The benefits of exercise involve skeletal muscle redox state alterations of nicotinamide adenine dinucleotide (NAD) and flavin adenine dinucleotide (FAD). We determined the fiber-specific effects of acute exercise on the skeletal muscle redox state in healthy adults. Muscle biopsies were obtained from 19 participants (11 M, 8 F; 26 ± 4 yr) at baseline (fasted) and 30 min and 3 h after treadmill exercise at 80% maximal oxygen consumption (V̇o(2max)). Muscle samples were probed for autofluorescence of NADH (excitation at 340–360 nm) and oxidized flavoproteins (Fp; excitation at 440–470 nm) and subsequently, fiber typed to quantify the redox signatures of individual muscle fibers. Redox state was calculated as the oxidation-to-reduction redox ratio: Fp/(Fp + NADH). At baseline, pair-wise comparisons revealed that the redox ratio of myosin heavy chain (MHC) I fibers was 7.2% higher than MHC IIa (P = 0.023, 95% CI: 5.2, 9.2%) and the redox ratio of MHC IIa was 8.0% higher than MHC IIx (P = 0.035, 95% CI: 6.8, 9.2%). MHC I fibers also displayed greater NADH intensity than MHC IIx (P = 0.007) and greater Fp intensity than both MHC IIa (P = 0.019) and MHC IIx (P < 0.0001). Fp intensities increased in all fiber types (main effect, P = 0.039) but redox ratios did not change (main effect, P = 0.483) 30 min after exercise. The change in redox ratio was positively correlated with capillary density in MHC I (rho = 0.762, P = 0.037), MHC IIa fibers (rho = 0.881, P = 0.007), and modestly in MHC IIx fibers (rho = 0. 771, P = 0.103). These findings support the use of redox autofluorescence to interrogate skeletal muscle metabolism. NEW & NOTEWORTHY This study is the first to use autofluorescent imaging to describe differential redox states within human skeletal muscle fiber types with exercise. Our findings highlight an easy and efficacious technique for assessing skeletal muscle redox in humans.
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spelling pubmed-105389952023-09-29 Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity Shadiow, James Miranda, Edwin R. Perkins, Ryan K. Mazo, Corey E. Lin, Zhen Lewis, Kendell N. Mey, Jacob T. Solomon, Thomas P. J. Haus, Jacob M. J Appl Physiol (1985) Research Article The benefits of exercise involve skeletal muscle redox state alterations of nicotinamide adenine dinucleotide (NAD) and flavin adenine dinucleotide (FAD). We determined the fiber-specific effects of acute exercise on the skeletal muscle redox state in healthy adults. Muscle biopsies were obtained from 19 participants (11 M, 8 F; 26 ± 4 yr) at baseline (fasted) and 30 min and 3 h after treadmill exercise at 80% maximal oxygen consumption (V̇o(2max)). Muscle samples were probed for autofluorescence of NADH (excitation at 340–360 nm) and oxidized flavoproteins (Fp; excitation at 440–470 nm) and subsequently, fiber typed to quantify the redox signatures of individual muscle fibers. Redox state was calculated as the oxidation-to-reduction redox ratio: Fp/(Fp + NADH). At baseline, pair-wise comparisons revealed that the redox ratio of myosin heavy chain (MHC) I fibers was 7.2% higher than MHC IIa (P = 0.023, 95% CI: 5.2, 9.2%) and the redox ratio of MHC IIa was 8.0% higher than MHC IIx (P = 0.035, 95% CI: 6.8, 9.2%). MHC I fibers also displayed greater NADH intensity than MHC IIx (P = 0.007) and greater Fp intensity than both MHC IIa (P = 0.019) and MHC IIx (P < 0.0001). Fp intensities increased in all fiber types (main effect, P = 0.039) but redox ratios did not change (main effect, P = 0.483) 30 min after exercise. The change in redox ratio was positively correlated with capillary density in MHC I (rho = 0.762, P = 0.037), MHC IIa fibers (rho = 0.881, P = 0.007), and modestly in MHC IIx fibers (rho = 0. 771, P = 0.103). These findings support the use of redox autofluorescence to interrogate skeletal muscle metabolism. NEW & NOTEWORTHY This study is the first to use autofluorescent imaging to describe differential redox states within human skeletal muscle fiber types with exercise. Our findings highlight an easy and efficacious technique for assessing skeletal muscle redox in humans. American Physiological Society 2023-09-01 2023-07-20 /pmc/articles/PMC10538995/ /pubmed/37471216 http://dx.doi.org/10.1152/japplphysiol.00662.2022 Text en Copyright © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Licensed under Creative Commons Attribution CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/) . Published by the American Physiological Society.
spellingShingle Research Article
Shadiow, James
Miranda, Edwin R.
Perkins, Ryan K.
Mazo, Corey E.
Lin, Zhen
Lewis, Kendell N.
Mey, Jacob T.
Solomon, Thomas P. J.
Haus, Jacob M.
Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
title Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
title_full Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
title_fullStr Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
title_full_unstemmed Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
title_short Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
title_sort exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538995/
https://www.ncbi.nlm.nih.gov/pubmed/37471216
http://dx.doi.org/10.1152/japplphysiol.00662.2022
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