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Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance...

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Autores principales: Li, Hongna, Hua, Lei, Zhao, Shuqing, Hao, Ming, Song, Rui, Pang, Shuyong, Liu, Yanna, Chen, Hong, Zhang, Wenjun, Shen, Tao, Gou, Jin-Ying, Mao, Hailiang, Wang, Guiping, Hao, Xiaohua, Li, Jian, Song, Baoxing, Lan, Caixia, Li, Zaifeng, Deng, Xing Wang, Dubcovsky, Jorge, Wang, Xiaodong, Chen, Shisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539295/
https://www.ncbi.nlm.nih.gov/pubmed/37770474
http://dx.doi.org/10.1038/s41467-023-41833-2
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author Li, Hongna
Hua, Lei
Zhao, Shuqing
Hao, Ming
Song, Rui
Pang, Shuyong
Liu, Yanna
Chen, Hong
Zhang, Wenjun
Shen, Tao
Gou, Jin-Ying
Mao, Hailiang
Wang, Guiping
Hao, Xiaohua
Li, Jian
Song, Baoxing
Lan, Caixia
Li, Zaifeng
Deng, Xing Wang
Dubcovsky, Jorge
Wang, Xiaodong
Chen, Shisheng
author_facet Li, Hongna
Hua, Lei
Zhao, Shuqing
Hao, Ming
Song, Rui
Pang, Shuyong
Liu, Yanna
Chen, Hong
Zhang, Wenjun
Shen, Tao
Gou, Jin-Ying
Mao, Hailiang
Wang, Guiping
Hao, Xiaohua
Li, Jian
Song, Baoxing
Lan, Caixia
Li, Zaifeng
Deng, Xing Wang
Dubcovsky, Jorge
Wang, Xiaodong
Chen, Shisheng
author_sort Li, Hongna
collection PubMed
description Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides. Lr47 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. Lr47 introgression lines with no or reduced linkage drag are generated using the Pairing homoeologous1 mutation, and a diagnostic molecular marker for Lr47 is developed. The coiled-coil domain of the Lr47 protein is unable to induce cell death, nor does it have self-protein interaction. The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.
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spelling pubmed-105392952023-09-30 Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides Li, Hongna Hua, Lei Zhao, Shuqing Hao, Ming Song, Rui Pang, Shuyong Liu, Yanna Chen, Hong Zhang, Wenjun Shen, Tao Gou, Jin-Ying Mao, Hailiang Wang, Guiping Hao, Xiaohua Li, Jian Song, Baoxing Lan, Caixia Li, Zaifeng Deng, Xing Wang Dubcovsky, Jorge Wang, Xiaodong Chen, Shisheng Nat Commun Article Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides. Lr47 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. Lr47 introgression lines with no or reduced linkage drag are generated using the Pairing homoeologous1 mutation, and a diagnostic molecular marker for Lr47 is developed. The coiled-coil domain of the Lr47 protein is unable to induce cell death, nor does it have self-protein interaction. The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease. Nature Publishing Group UK 2023-09-28 /pmc/articles/PMC10539295/ /pubmed/37770474 http://dx.doi.org/10.1038/s41467-023-41833-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Hongna
Hua, Lei
Zhao, Shuqing
Hao, Ming
Song, Rui
Pang, Shuyong
Liu, Yanna
Chen, Hong
Zhang, Wenjun
Shen, Tao
Gou, Jin-Ying
Mao, Hailiang
Wang, Guiping
Hao, Xiaohua
Li, Jian
Song, Baoxing
Lan, Caixia
Li, Zaifeng
Deng, Xing Wang
Dubcovsky, Jorge
Wang, Xiaodong
Chen, Shisheng
Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
title Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
title_full Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
title_fullStr Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
title_full_unstemmed Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
title_short Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
title_sort cloning of the wheat leaf rust resistance gene lr47 introgressed from aegilops speltoides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539295/
https://www.ncbi.nlm.nih.gov/pubmed/37770474
http://dx.doi.org/10.1038/s41467-023-41833-2
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