Cargando…
Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2
INTRODUCTION: Dengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539573/ https://www.ncbi.nlm.nih.gov/pubmed/37779723 http://dx.doi.org/10.3389/fmicb.2023.1260812 |
_version_ | 1785113529268830208 |
---|---|
author | Sarma, Devojit Kumar Rathod, Lokendra Mishra, Sweta Das, Deepanker Agarwal, Ankita Sharma, Gaurav Singh, Tanim Arpit Kumawat, Manoj Singh, Samradhi Verma, Vinod Kumar, Manoj Shubham, Swasti Tiwari, Rajnarayan R. Prakash, Anil |
author_facet | Sarma, Devojit Kumar Rathod, Lokendra Mishra, Sweta Das, Deepanker Agarwal, Ankita Sharma, Gaurav Singh, Tanim Arpit Kumawat, Manoj Singh, Samradhi Verma, Vinod Kumar, Manoj Shubham, Swasti Tiwari, Rajnarayan R. Prakash, Anil |
author_sort | Sarma, Devojit Kumar |
collection | PubMed |
description | INTRODUCTION: Dengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases. OBJECTIVES: The present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected Aedes mosquitoes in Bhopal, Central India. METHODS: Aedes mosquitoes were collected from 29 localities within Bhopal city during October 2020 to September 2022. DENV infection was assessed in the individual head and thorax regions of Aedes mosquitoes using reverse transcriptase PCR. Positive samples were sequenced, and the circulating serotypes and genotypes were determined using phylogenetic analysis. RESULTS: DENV RNA was detected in 7 Aedes aegypti and 1 Aedes albopictus, with infection rates of 0.59 and 0.14%, respectively. Phylogenetic analysis revealed all the isolates belonged to DENV serotype 2 and distinctly clustered with the non-Indian lineage (cosmopolitan genotype 4a), which was not recorded from the study area earlier. The time to most common recent ancestor (TMRCA) of these sequences was 7.4 years old, with the highest posterior density (HPD) of 3.5–12.2 years, indicating that this new lineage emerged during the year 2014. This is the first report on the DENV incrimination in both Ae. aegypti and Ae. albopictus mosquitoes collected from Bhopal, Central India. CONCLUSION: The observed emergence of the non-Indian lineage of DENV-2 in Bhopal, which again is a first report from the area, coincides with the gradual increase in DENV cases in Bhopal since 2014. This study emphasizes the importance of DENV surveillance and risk assessment in this strategically important part of the country to decipher its outbreak and severe disease-causing potential. |
format | Online Article Text |
id | pubmed-10539573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105395732023-09-30 Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 Sarma, Devojit Kumar Rathod, Lokendra Mishra, Sweta Das, Deepanker Agarwal, Ankita Sharma, Gaurav Singh, Tanim Arpit Kumawat, Manoj Singh, Samradhi Verma, Vinod Kumar, Manoj Shubham, Swasti Tiwari, Rajnarayan R. Prakash, Anil Front Microbiol Microbiology INTRODUCTION: Dengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases. OBJECTIVES: The present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected Aedes mosquitoes in Bhopal, Central India. METHODS: Aedes mosquitoes were collected from 29 localities within Bhopal city during October 2020 to September 2022. DENV infection was assessed in the individual head and thorax regions of Aedes mosquitoes using reverse transcriptase PCR. Positive samples were sequenced, and the circulating serotypes and genotypes were determined using phylogenetic analysis. RESULTS: DENV RNA was detected in 7 Aedes aegypti and 1 Aedes albopictus, with infection rates of 0.59 and 0.14%, respectively. Phylogenetic analysis revealed all the isolates belonged to DENV serotype 2 and distinctly clustered with the non-Indian lineage (cosmopolitan genotype 4a), which was not recorded from the study area earlier. The time to most common recent ancestor (TMRCA) of these sequences was 7.4 years old, with the highest posterior density (HPD) of 3.5–12.2 years, indicating that this new lineage emerged during the year 2014. This is the first report on the DENV incrimination in both Ae. aegypti and Ae. albopictus mosquitoes collected from Bhopal, Central India. CONCLUSION: The observed emergence of the non-Indian lineage of DENV-2 in Bhopal, which again is a first report from the area, coincides with the gradual increase in DENV cases in Bhopal since 2014. This study emphasizes the importance of DENV surveillance and risk assessment in this strategically important part of the country to decipher its outbreak and severe disease-causing potential. Frontiers Media S.A. 2023-09-14 /pmc/articles/PMC10539573/ /pubmed/37779723 http://dx.doi.org/10.3389/fmicb.2023.1260812 Text en Copyright © 2023 Sarma, Rathod, Mishra, Das, Agarwal, Sharma, Singh, Kumawat, Singh, Verma, Kumar, Shubham, Tiwari and Prakash. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Sarma, Devojit Kumar Rathod, Lokendra Mishra, Sweta Das, Deepanker Agarwal, Ankita Sharma, Gaurav Singh, Tanim Arpit Kumawat, Manoj Singh, Samradhi Verma, Vinod Kumar, Manoj Shubham, Swasti Tiwari, Rajnarayan R. Prakash, Anil Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
title | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
title_full | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
title_fullStr | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
title_full_unstemmed | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
title_short | Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2 |
title_sort | molecular surveillance of dengue virus in field-collected aedes mosquitoes from bhopal, central india: evidence of circulation of a new lineage of serotype 2 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539573/ https://www.ncbi.nlm.nih.gov/pubmed/37779723 http://dx.doi.org/10.3389/fmicb.2023.1260812 |
work_keys_str_mv | AT sarmadevojitkumar molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT rathodlokendra molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT mishrasweta molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT dasdeepanker molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT agarwalankita molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT sharmagaurav molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT singhtanimarpit molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT kumawatmanoj molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT singhsamradhi molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT vermavinod molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT kumarmanoj molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT shubhamswasti molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT tiwarirajnarayanr molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 AT prakashanil molecularsurveillanceofdenguevirusinfieldcollectedaedesmosquitoesfrombhopalcentralindiaevidenceofcirculationofanewlineageofserotype2 |