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The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles

RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi con...

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Autores principales: Dallastella, Marianna, de Oliveira, Willian Klassen, Rodrigues, Marcio L., Goldenberg, Samuel, Alves, Lysangela R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539620/
https://www.ncbi.nlm.nih.gov/pubmed/37780856
http://dx.doi.org/10.3389/fcimb.2023.1247329
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author Dallastella, Marianna
de Oliveira, Willian Klassen
Rodrigues, Marcio L.
Goldenberg, Samuel
Alves, Lysangela R.
author_facet Dallastella, Marianna
de Oliveira, Willian Klassen
Rodrigues, Marcio L.
Goldenberg, Samuel
Alves, Lysangela R.
author_sort Dallastella, Marianna
collection PubMed
description RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs. Proteins with canonical RNA-binding domains interact with proteins and RNA, such as the RNA Recognition Motif (RRM), Zinc finger, and hnRNP K-homology (KH) domains. Polyadenylate-binding protein (PABP) plays a critical role in the regulation of gene expression by binding the poly(A) tail of messenger RNA (mRNA) and facilitating its translation, stability, and localization, making it a key factor in post-transcriptional control of gene expression. The presence of proteins related to the RNA life cycle in EVs from different fungal species suggests a conserved mechanism of EV cargo packing. Various models have been proposed for selecting RNA molecules for release into EVs. Still, the actual loading processes are unknown, and further molecular characterization of these proteins may provide insight into the mechanism of RNA sorting into EVs. This work reviews the current knowledge of RBPs and proteins related to RNA metabolism in EVs derived from distinct fungi species, and presents an analysis of proteomic datasets through GO term and orthology analysis, Our investigation identified orthologous proteins in fungal EVs on different fungal species.
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spelling pubmed-105396202023-09-30 The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles Dallastella, Marianna de Oliveira, Willian Klassen Rodrigues, Marcio L. Goldenberg, Samuel Alves, Lysangela R. Front Cell Infect Microbiol Cellular and Infection Microbiology RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs. Proteins with canonical RNA-binding domains interact with proteins and RNA, such as the RNA Recognition Motif (RRM), Zinc finger, and hnRNP K-homology (KH) domains. Polyadenylate-binding protein (PABP) plays a critical role in the regulation of gene expression by binding the poly(A) tail of messenger RNA (mRNA) and facilitating its translation, stability, and localization, making it a key factor in post-transcriptional control of gene expression. The presence of proteins related to the RNA life cycle in EVs from different fungal species suggests a conserved mechanism of EV cargo packing. Various models have been proposed for selecting RNA molecules for release into EVs. Still, the actual loading processes are unknown, and further molecular characterization of these proteins may provide insight into the mechanism of RNA sorting into EVs. This work reviews the current knowledge of RBPs and proteins related to RNA metabolism in EVs derived from distinct fungi species, and presents an analysis of proteomic datasets through GO term and orthology analysis, Our investigation identified orthologous proteins in fungal EVs on different fungal species. Frontiers Media S.A. 2023-09-14 /pmc/articles/PMC10539620/ /pubmed/37780856 http://dx.doi.org/10.3389/fcimb.2023.1247329 Text en Copyright © 2023 Dallastella, Oliveira, Rodrigues, Goldenberg and Alves https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Dallastella, Marianna
de Oliveira, Willian Klassen
Rodrigues, Marcio L.
Goldenberg, Samuel
Alves, Lysangela R.
The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
title The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
title_full The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
title_fullStr The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
title_full_unstemmed The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
title_short The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
title_sort characterization of rna-binding proteins and rna metabolism-related proteins in fungal extracellular vesicles
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539620/
https://www.ncbi.nlm.nih.gov/pubmed/37780856
http://dx.doi.org/10.3389/fcimb.2023.1247329
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