Cargando…

Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect

Tetrastigma hemsleyanum (Tetrastigma hemsleyanum Diels et Gilg) is a valuable traditional Chinese medicine with various applications. In this study, we aimed to optimize the extraction process for the total extraction yield of five flavonoid components, namely kaempferol, quercetin, rutin, kaempfero...

Descripción completa

Detalles Bibliográficos
Autores principales: Shu, Jianhao, Zhao, Yali, Zhou, Yehui, Lin, Feifei, Song, Jingmei, Li, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539637/
https://www.ncbi.nlm.nih.gov/pubmed/37780783
http://dx.doi.org/10.1016/j.heliyon.2023.e20200
_version_ 1785113544503590912
author Shu, Jianhao
Zhao, Yali
Zhou, Yehui
Lin, Feifei
Song, Jingmei
Li, Xiaohong
author_facet Shu, Jianhao
Zhao, Yali
Zhou, Yehui
Lin, Feifei
Song, Jingmei
Li, Xiaohong
author_sort Shu, Jianhao
collection PubMed
description Tetrastigma hemsleyanum (Tetrastigma hemsleyanum Diels et Gilg) is a valuable traditional Chinese medicine with various applications. In this study, we aimed to optimize the extraction process for the total extraction yield of five flavonoid components, namely kaempferol, quercetin, rutin, kaempferol-3-O-rutinoside, and astragalin from the Tetrastigma hemsleyanum root (THR), and explore its potential molecular mechanisms in treating oxidative diseases as well as antioxidant activity. To achieve these objectives, we employed the genetic algorithm-back propagation neural network (GA-BPNN), the Box-Behnken design (BBD) with 4-factors and 3-levels to establish the optimal ethanol extraction process for the total extraction yield of the 5 components. Using public databases, the “component core targets-disease core target genes” networks were built, as well as molecular docking. Furthermore, DPPH was used to examine the antioxidant activity of the extracts obtained from THR under the optimal extraction process. The experimental value of the total extraction yield of the 5 components achieved a maximum of 788.12 mg/kg when the ethanol concentration was 73%, the solid-liquid ratio was 26 g/mL, and the ultrasonic duration was 30 min, and the ultrasonic temperature was 76 °C. When docked with protein molecules such as 6Y8I, quercetin, and other components received moderate to high scores. When the total concentration of the 5 components was 3.033 μg/mL, the DPPH radical scavenging rate was 89.81%. Compared with the BBD method, the GA-BPNN method is more efficient and reliable for optimizing the extraction process of active ingredients in THR because of its good data-fitting ability.
format Online
Article
Text
id pubmed-10539637
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-105396372023-09-30 Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect Shu, Jianhao Zhao, Yali Zhou, Yehui Lin, Feifei Song, Jingmei Li, Xiaohong Heliyon Research Article Tetrastigma hemsleyanum (Tetrastigma hemsleyanum Diels et Gilg) is a valuable traditional Chinese medicine with various applications. In this study, we aimed to optimize the extraction process for the total extraction yield of five flavonoid components, namely kaempferol, quercetin, rutin, kaempferol-3-O-rutinoside, and astragalin from the Tetrastigma hemsleyanum root (THR), and explore its potential molecular mechanisms in treating oxidative diseases as well as antioxidant activity. To achieve these objectives, we employed the genetic algorithm-back propagation neural network (GA-BPNN), the Box-Behnken design (BBD) with 4-factors and 3-levels to establish the optimal ethanol extraction process for the total extraction yield of the 5 components. Using public databases, the “component core targets-disease core target genes” networks were built, as well as molecular docking. Furthermore, DPPH was used to examine the antioxidant activity of the extracts obtained from THR under the optimal extraction process. The experimental value of the total extraction yield of the 5 components achieved a maximum of 788.12 mg/kg when the ethanol concentration was 73%, the solid-liquid ratio was 26 g/mL, and the ultrasonic duration was 30 min, and the ultrasonic temperature was 76 °C. When docked with protein molecules such as 6Y8I, quercetin, and other components received moderate to high scores. When the total concentration of the 5 components was 3.033 μg/mL, the DPPH radical scavenging rate was 89.81%. Compared with the BBD method, the GA-BPNN method is more efficient and reliable for optimizing the extraction process of active ingredients in THR because of its good data-fitting ability. Elsevier 2023-09-22 /pmc/articles/PMC10539637/ /pubmed/37780783 http://dx.doi.org/10.1016/j.heliyon.2023.e20200 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Shu, Jianhao
Zhao, Yali
Zhou, Yehui
Lin, Feifei
Song, Jingmei
Li, Xiaohong
Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect
title Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect
title_full Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect
title_fullStr Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect
title_full_unstemmed Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect
title_short Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect
title_sort optimization of tetrastigma hemsleyanum extraction process based on ga-bpnn model and analysis of its antioxidant effect
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539637/
https://www.ncbi.nlm.nih.gov/pubmed/37780783
http://dx.doi.org/10.1016/j.heliyon.2023.e20200
work_keys_str_mv AT shujianhao optimizationoftetrastigmahemsleyanumextractionprocessbasedongabpnnmodelandanalysisofitsantioxidanteffect
AT zhaoyali optimizationoftetrastigmahemsleyanumextractionprocessbasedongabpnnmodelandanalysisofitsantioxidanteffect
AT zhouyehui optimizationoftetrastigmahemsleyanumextractionprocessbasedongabpnnmodelandanalysisofitsantioxidanteffect
AT linfeifei optimizationoftetrastigmahemsleyanumextractionprocessbasedongabpnnmodelandanalysisofitsantioxidanteffect
AT songjingmei optimizationoftetrastigmahemsleyanumextractionprocessbasedongabpnnmodelandanalysisofitsantioxidanteffect
AT lixiaohong optimizationoftetrastigmahemsleyanumextractionprocessbasedongabpnnmodelandanalysisofitsantioxidanteffect