(129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer

The availability of a benchtop nuclear magnetic resonance (NMR) spectrometer, of low cost and easily transportable, can allow detection of low quantities of biosensors, provided that hyperpolarized species are used. Here we show that the micromolar threshold can easily be reached by employing laser-...

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Autores principales: Chighine, Kévin, Léonce, Estelle, Boutin, Céline, Desvaux, Hervé, Berthault, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Copernicus GmbH 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539730/
https://www.ncbi.nlm.nih.gov/pubmed/37904767
http://dx.doi.org/10.5194/mr-2-409-2021
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author Chighine, Kévin
Léonce, Estelle
Boutin, Céline
Desvaux, Hervé
Berthault, Patrick
author_facet Chighine, Kévin
Léonce, Estelle
Boutin, Céline
Desvaux, Hervé
Berthault, Patrick
author_sort Chighine, Kévin
collection PubMed
description The availability of a benchtop nuclear magnetic resonance (NMR) spectrometer, of low cost and easily transportable, can allow detection of low quantities of biosensors, provided that hyperpolarized species are used. Here we show that the micromolar threshold can easily be reached by employing laser-polarized xenon and cage molecules reversibly hosting it. Indirect detection of caged xenon is made via chemical exchange, using ultra-fast Z spectroscopy based on spatio-temporal encoding. On this non-dedicated low-field spectrometer, several ideas are proposed to improve the signal.
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spelling pubmed-105397302023-10-30 (129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer Chighine, Kévin Léonce, Estelle Boutin, Céline Desvaux, Hervé Berthault, Patrick Magn Reson (Gott) Research Article The availability of a benchtop nuclear magnetic resonance (NMR) spectrometer, of low cost and easily transportable, can allow detection of low quantities of biosensors, provided that hyperpolarized species are used. Here we show that the micromolar threshold can easily be reached by employing laser-polarized xenon and cage molecules reversibly hosting it. Indirect detection of caged xenon is made via chemical exchange, using ultra-fast Z spectroscopy based on spatio-temporal encoding. On this non-dedicated low-field spectrometer, several ideas are proposed to improve the signal. Copernicus GmbH 2021-06-11 /pmc/articles/PMC10539730/ /pubmed/37904767 http://dx.doi.org/10.5194/mr-2-409-2021 Text en Copyright: © 2021 Kévin Chighine et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/
spellingShingle Research Article
Chighine, Kévin
Léonce, Estelle
Boutin, Céline
Desvaux, Hervé
Berthault, Patrick
(129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer
title (129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer
title_full (129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer
title_fullStr (129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer
title_full_unstemmed (129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer
title_short (129)Xe ultra-fast Z spectroscopy enables micromolar detection of biosensors on a 1 T benchtop spectrometer
title_sort (129)xe ultra-fast z spectroscopy enables micromolar detection of biosensors on a 1 t benchtop spectrometer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539730/
https://www.ncbi.nlm.nih.gov/pubmed/37904767
http://dx.doi.org/10.5194/mr-2-409-2021
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