Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis

Here, we present a protocol for exploring the effects of PPP1R15A inhibitor, Sephin1, on antitumor immunity of B16F1 subcutaneous tumor in mice. We describe steps for constructing single-cell transcriptome and TCR libraries, sequencing, and using sequencing data for the integration of expression and...

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Detalles Bibliográficos
Autores principales: Wang, Rongjing, Wang, Minghui, Pei, Siyu, Zhang, Yuchao, Guo, Shiwei, Guo, Wei, Wu, Zhenchuan, Wang, Hailong, Li, Yizhe, Zhu, Yufei, Meng, Ling-Hua, Lang, Jingyu, Jin, Gang, Xiao, Yichuan, Hu, Landian, Kong, Xiangyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539956/
https://www.ncbi.nlm.nih.gov/pubmed/37756156
http://dx.doi.org/10.1016/j.xpro.2023.102616
Descripción
Sumario:Here, we present a protocol for exploring the effects of PPP1R15A inhibitor, Sephin1, on antitumor immunity of B16F1 subcutaneous tumor in mice. We describe steps for constructing single-cell transcriptome and TCR libraries, sequencing, and using sequencing data for the integration of expression and TCR data. We then detail procedures for gene differentiation, regulon and cell-cell communication analysis, and validation of single-cell analysis results. For complete details on the use and execution of this protocol, please refer to Wang et al.(1)

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