Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis

The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe s...

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Detalles Bibliográficos
Autores principales: Bafna, Akanksha, Lau, Petrina, Banks, Gareth, Nolan, Patrick M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539961/
https://www.ncbi.nlm.nih.gov/pubmed/37756154
http://dx.doi.org/10.1016/j.xpro.2023.102618
Descripción
Sumario:The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies.

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