Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis

The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe s...

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Detalles Bibliográficos
Autores principales: Bafna, Akanksha, Lau, Petrina, Banks, Gareth, Nolan, Patrick M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539961/
https://www.ncbi.nlm.nih.gov/pubmed/37756154
http://dx.doi.org/10.1016/j.xpro.2023.102618
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author Bafna, Akanksha
Lau, Petrina
Banks, Gareth
Nolan, Patrick M.
author_facet Bafna, Akanksha
Lau, Petrina
Banks, Gareth
Nolan, Patrick M.
author_sort Bafna, Akanksha
collection PubMed
description The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies.
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spelling pubmed-105399612023-09-30 Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis Bafna, Akanksha Lau, Petrina Banks, Gareth Nolan, Patrick M. STAR Protoc Protocol The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies. Elsevier 2023-09-26 /pmc/articles/PMC10539961/ /pubmed/37756154 http://dx.doi.org/10.1016/j.xpro.2023.102618 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Bafna, Akanksha
Lau, Petrina
Banks, Gareth
Nolan, Patrick M.
Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
title Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
title_full Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
title_fullStr Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
title_full_unstemmed Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
title_short Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
title_sort harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539961/
https://www.ncbi.nlm.nih.gov/pubmed/37756154
http://dx.doi.org/10.1016/j.xpro.2023.102618
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