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pH-Responsive Elastin-Like Polypeptide Designer Condensates

[Image: see text] Biomolecular condensates are macromolecular complexes formed by liquid–liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial...

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Autores principales: de Haas, Robbert J., Ganar, Ketan A., Deshpande, Siddharth, de Vries, Renko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10540133/
https://www.ncbi.nlm.nih.gov/pubmed/37707425
http://dx.doi.org/10.1021/acsami.3c11314
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author de Haas, Robbert J.
Ganar, Ketan A.
Deshpande, Siddharth
de Vries, Renko
author_facet de Haas, Robbert J.
Ganar, Ketan A.
Deshpande, Siddharth
de Vries, Renko
author_sort de Haas, Robbert J.
collection PubMed
description [Image: see text] Biomolecular condensates are macromolecular complexes formed by liquid–liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that are able to mimic spatiotemporal control over the biochemical reactions. Here, we design and test a family of condensate-forming, pH-responsive elastin-like polypeptides (ELPs) that form condensates above critical pH values ranging between 4 and 7, for temperatures between 20 and at 37 °C. We show that the condensation occurs rapidly, in sharp pH intervals (ΔpH < 0.3). For eventual applications in engineering synthetic cell compartments, we demonstrate that multiple types of pH-responsive ELPs can form mixed condensates inside micron-sized vesicles. When genetically fused with enzymes, receptors, and signaling molecules, these pH-responsive ELPs could be potentially used as pH-switchable functional condensates for spatially controlling biochemistry in engineered synthetic cells.
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spelling pubmed-105401332023-09-30 pH-Responsive Elastin-Like Polypeptide Designer Condensates de Haas, Robbert J. Ganar, Ketan A. Deshpande, Siddharth de Vries, Renko ACS Appl Mater Interfaces [Image: see text] Biomolecular condensates are macromolecular complexes formed by liquid–liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that are able to mimic spatiotemporal control over the biochemical reactions. Here, we design and test a family of condensate-forming, pH-responsive elastin-like polypeptides (ELPs) that form condensates above critical pH values ranging between 4 and 7, for temperatures between 20 and at 37 °C. We show that the condensation occurs rapidly, in sharp pH intervals (ΔpH < 0.3). For eventual applications in engineering synthetic cell compartments, we demonstrate that multiple types of pH-responsive ELPs can form mixed condensates inside micron-sized vesicles. When genetically fused with enzymes, receptors, and signaling molecules, these pH-responsive ELPs could be potentially used as pH-switchable functional condensates for spatially controlling biochemistry in engineered synthetic cells. American Chemical Society 2023-09-14 /pmc/articles/PMC10540133/ /pubmed/37707425 http://dx.doi.org/10.1021/acsami.3c11314 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle de Haas, Robbert J.
Ganar, Ketan A.
Deshpande, Siddharth
de Vries, Renko
pH-Responsive Elastin-Like Polypeptide Designer Condensates
title pH-Responsive Elastin-Like Polypeptide Designer Condensates
title_full pH-Responsive Elastin-Like Polypeptide Designer Condensates
title_fullStr pH-Responsive Elastin-Like Polypeptide Designer Condensates
title_full_unstemmed pH-Responsive Elastin-Like Polypeptide Designer Condensates
title_short pH-Responsive Elastin-Like Polypeptide Designer Condensates
title_sort ph-responsive elastin-like polypeptide designer condensates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10540133/
https://www.ncbi.nlm.nih.gov/pubmed/37707425
http://dx.doi.org/10.1021/acsami.3c11314
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