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pH-Responsive Elastin-Like Polypeptide Designer Condensates
[Image: see text] Biomolecular condensates are macromolecular complexes formed by liquid–liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10540133/ https://www.ncbi.nlm.nih.gov/pubmed/37707425 http://dx.doi.org/10.1021/acsami.3c11314 |
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author | de Haas, Robbert J. Ganar, Ketan A. Deshpande, Siddharth de Vries, Renko |
author_facet | de Haas, Robbert J. Ganar, Ketan A. Deshpande, Siddharth de Vries, Renko |
author_sort | de Haas, Robbert J. |
collection | PubMed |
description | [Image: see text] Biomolecular condensates are macromolecular complexes formed by liquid–liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that are able to mimic spatiotemporal control over the biochemical reactions. Here, we design and test a family of condensate-forming, pH-responsive elastin-like polypeptides (ELPs) that form condensates above critical pH values ranging between 4 and 7, for temperatures between 20 and at 37 °C. We show that the condensation occurs rapidly, in sharp pH intervals (ΔpH < 0.3). For eventual applications in engineering synthetic cell compartments, we demonstrate that multiple types of pH-responsive ELPs can form mixed condensates inside micron-sized vesicles. When genetically fused with enzymes, receptors, and signaling molecules, these pH-responsive ELPs could be potentially used as pH-switchable functional condensates for spatially controlling biochemistry in engineered synthetic cells. |
format | Online Article Text |
id | pubmed-10540133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105401332023-09-30 pH-Responsive Elastin-Like Polypeptide Designer Condensates de Haas, Robbert J. Ganar, Ketan A. Deshpande, Siddharth de Vries, Renko ACS Appl Mater Interfaces [Image: see text] Biomolecular condensates are macromolecular complexes formed by liquid–liquid phase separation. They regulate key biological functions by reversibly compartmentalizing molecules in cells, in a stimulus-dependent manner. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that are able to mimic spatiotemporal control over the biochemical reactions. Here, we design and test a family of condensate-forming, pH-responsive elastin-like polypeptides (ELPs) that form condensates above critical pH values ranging between 4 and 7, for temperatures between 20 and at 37 °C. We show that the condensation occurs rapidly, in sharp pH intervals (ΔpH < 0.3). For eventual applications in engineering synthetic cell compartments, we demonstrate that multiple types of pH-responsive ELPs can form mixed condensates inside micron-sized vesicles. When genetically fused with enzymes, receptors, and signaling molecules, these pH-responsive ELPs could be potentially used as pH-switchable functional condensates for spatially controlling biochemistry in engineered synthetic cells. American Chemical Society 2023-09-14 /pmc/articles/PMC10540133/ /pubmed/37707425 http://dx.doi.org/10.1021/acsami.3c11314 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | de Haas, Robbert J. Ganar, Ketan A. Deshpande, Siddharth de Vries, Renko pH-Responsive Elastin-Like Polypeptide Designer Condensates |
title | pH-Responsive
Elastin-Like Polypeptide Designer Condensates |
title_full | pH-Responsive
Elastin-Like Polypeptide Designer Condensates |
title_fullStr | pH-Responsive
Elastin-Like Polypeptide Designer Condensates |
title_full_unstemmed | pH-Responsive
Elastin-Like Polypeptide Designer Condensates |
title_short | pH-Responsive
Elastin-Like Polypeptide Designer Condensates |
title_sort | ph-responsive
elastin-like polypeptide designer condensates |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10540133/ https://www.ncbi.nlm.nih.gov/pubmed/37707425 http://dx.doi.org/10.1021/acsami.3c11314 |
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