Effect of Using Ethanol Extract of Artocarpus Heterophyllus Leaves and Olea Europea Fruit Oil Combination on Facial Skin

BACKGROUND: Artocarpus heterophyllus (A. heterophyllus) leaves and Olea europea (OE) fruit oil are natural sources that have been traditionally used for health and skin care purpose. OBJECTIVE: To assess the potential synergistic effect of combining ethanol extract of A. heterophyllus leaves (AHLE) and OE fruit oil in the formulation of clay masks, specifically in terms of their effect on facial skin. METHODS: AHLE was obtained by the maceration method, while OE was purchased commercially. Total phenol and flavonoid content were calculated and a DPPH assay was conducted to evaluate the antioxidant properties. Furthermore, the four formulas prepared were F1 (AHLE 5%), F2 (OE 10%), F3 (AHLE 5% + OE 10%), and F4 (AHLE 2.5% + OE 5%). Adult women received weekly facial treatments with the formulated mask for one month. The effect of these treatments was evaluated based on several skin parameters, including moisture, oiliness, texture, collagen levels, pigmentation, sensitivity, and the presence of wrinkles. Furthermore, the data obtained were analyzed using the Wilcoxon sign-ranked test. RESULTS: AHLE contained total phenolic, flavonoid, and antioxidant activity higher than OE. Clay masks in all formulations showed homogeneity and do not contain coarse grain. After four weeks of treatment, the efficacy of the formulations demonstrated a significant effect. F1 exhibited a reduction in wrinkles by 36.27%, while F3 improved oily skin by 21.39%, enhanced skin texture by 44.32%, reduced pigmentation by 30.30%, and decreased skin sensitivity by 49.18%. Furthermore, F4 demonstrated an increase in skin moisture levels by 27.89% and a boost in collagen production by 32.00%. CONCLUSION: The combination of AHLE and OE at 5% and 10% demonstrated superior effectiveness compared to their individual use.