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Engineered transcription-associated Cas9 targeting in eukaryotic cells

DNA targeting Class 2 CRISPR-Cas effector nucleases, including the well-studied Cas9 proteins, evolved protospacer-adjacent motif (PAM) and guide RNA interactions that sequentially license their binding and cleavage activities at protospacer target sites. Both interactions are nucleic acid sequence...

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Detalles Bibliográficos
Autores principales:	Goldberg, Gregory W., Kogenaru, Manjunatha, Keegan, Sarah, Haase, Max A. B., Kagermazova, Larisa, Arias, Mauricio A., Onyebeke, Kenenna, Adams, Samantha, Fenyö, David, Noyes, Marcus B., Boeke, Jef D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541143/ https://www.ncbi.nlm.nih.gov/pubmed/37781609 http://dx.doi.org/10.1101/2023.09.18.558319

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