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The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals

Hydractinia is a colonial marine hydroid that exhibits remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the gen...

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Autores principales: Schnitzler, Christine E., Chang, E. Sally, Waletich, Justin, Quiroga-Artigas, Gonzalo, Wong, Wai Yee, Nguyen, Anh-Dao, Barreira, Sofia N., Doonan, Liam, Gonzalez, Paul, Koren, Sergey, Gahan, James M., Sanders, Steven M., Bradshaw, Brian, DuBuc, Timothy Q., Febrimarsa, de Jong, Danielle, Nawrocki, Eric P., Larson, Alexandra, Klasfeld, Samantha, Gornik, Sebastian G., Moreland, R. Travis, Wolfsberg, Tyra G., Phillippy, Adam M., Mullikin, James C., Simakov, Oleg, Cartwright, Paulyn, Nicotra, Matthew, Frank, Uri, Baxevanis, Andreas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541594/
https://www.ncbi.nlm.nih.gov/pubmed/37786714
http://dx.doi.org/10.1101/2023.08.25.554815
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author Schnitzler, Christine E.
Chang, E. Sally
Waletich, Justin
Quiroga-Artigas, Gonzalo
Wong, Wai Yee
Nguyen, Anh-Dao
Barreira, Sofia N.
Doonan, Liam
Gonzalez, Paul
Koren, Sergey
Gahan, James M.
Sanders, Steven M.
Bradshaw, Brian
DuBuc, Timothy Q.
Febrimarsa
de Jong, Danielle
Nawrocki, Eric P.
Larson, Alexandra
Klasfeld, Samantha
Gornik, Sebastian G.
Moreland, R. Travis
Wolfsberg, Tyra G.
Phillippy, Adam M.
Mullikin, James C.
Simakov, Oleg
Cartwright, Paulyn
Nicotra, Matthew
Frank, Uri
Baxevanis, Andreas D.
author_facet Schnitzler, Christine E.
Chang, E. Sally
Waletich, Justin
Quiroga-Artigas, Gonzalo
Wong, Wai Yee
Nguyen, Anh-Dao
Barreira, Sofia N.
Doonan, Liam
Gonzalez, Paul
Koren, Sergey
Gahan, James M.
Sanders, Steven M.
Bradshaw, Brian
DuBuc, Timothy Q.
Febrimarsa
de Jong, Danielle
Nawrocki, Eric P.
Larson, Alexandra
Klasfeld, Samantha
Gornik, Sebastian G.
Moreland, R. Travis
Wolfsberg, Tyra G.
Phillippy, Adam M.
Mullikin, James C.
Simakov, Oleg
Cartwright, Paulyn
Nicotra, Matthew
Frank, Uri
Baxevanis, Andreas D.
author_sort Schnitzler, Christine E.
collection PubMed
description Hydractinia is a colonial marine hydroid that exhibits remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractinia species, H. symbiolongicarpus and H. echinata, placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpus and identified cell type markers for all major cell types, including key i-cell markers. Orthology analyses based on the markers revealed that Hydractinia’s i-cells are highly enriched in genes that are widely shared amongst animals, a striking finding given that Hydractinia has a higher proportion of phylum-specific genes than any of the other 41 animals in our orthology analysis. These results indicate that Hydractinia’s stem cells and early progenitor cells may use a toolkit shared with all animals, making it a promising model organism for future exploration of stem cell biology and regenerative medicine. The genomic and transcriptomic resources for Hydractinia presented here will enable further studies of their regenerative capacity, colonial morphology, and ability to distinguish self from non-self.
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spelling pubmed-105415942023-10-02 The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals Schnitzler, Christine E. Chang, E. Sally Waletich, Justin Quiroga-Artigas, Gonzalo Wong, Wai Yee Nguyen, Anh-Dao Barreira, Sofia N. Doonan, Liam Gonzalez, Paul Koren, Sergey Gahan, James M. Sanders, Steven M. Bradshaw, Brian DuBuc, Timothy Q. Febrimarsa de Jong, Danielle Nawrocki, Eric P. Larson, Alexandra Klasfeld, Samantha Gornik, Sebastian G. Moreland, R. Travis Wolfsberg, Tyra G. Phillippy, Adam M. Mullikin, James C. Simakov, Oleg Cartwright, Paulyn Nicotra, Matthew Frank, Uri Baxevanis, Andreas D. bioRxiv Article Hydractinia is a colonial marine hydroid that exhibits remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractinia species, H. symbiolongicarpus and H. echinata, placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpus and identified cell type markers for all major cell types, including key i-cell markers. Orthology analyses based on the markers revealed that Hydractinia’s i-cells are highly enriched in genes that are widely shared amongst animals, a striking finding given that Hydractinia has a higher proportion of phylum-specific genes than any of the other 41 animals in our orthology analysis. These results indicate that Hydractinia’s stem cells and early progenitor cells may use a toolkit shared with all animals, making it a promising model organism for future exploration of stem cell biology and regenerative medicine. The genomic and transcriptomic resources for Hydractinia presented here will enable further studies of their regenerative capacity, colonial morphology, and ability to distinguish self from non-self. Cold Spring Harbor Laboratory 2023-08-27 /pmc/articles/PMC10541594/ /pubmed/37786714 http://dx.doi.org/10.1101/2023.08.25.554815 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Schnitzler, Christine E.
Chang, E. Sally
Waletich, Justin
Quiroga-Artigas, Gonzalo
Wong, Wai Yee
Nguyen, Anh-Dao
Barreira, Sofia N.
Doonan, Liam
Gonzalez, Paul
Koren, Sergey
Gahan, James M.
Sanders, Steven M.
Bradshaw, Brian
DuBuc, Timothy Q.
Febrimarsa
de Jong, Danielle
Nawrocki, Eric P.
Larson, Alexandra
Klasfeld, Samantha
Gornik, Sebastian G.
Moreland, R. Travis
Wolfsberg, Tyra G.
Phillippy, Adam M.
Mullikin, James C.
Simakov, Oleg
Cartwright, Paulyn
Nicotra, Matthew
Frank, Uri
Baxevanis, Andreas D.
The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
title The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
title_full The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
title_fullStr The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
title_full_unstemmed The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
title_short The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
title_sort genome of the colonial hydroid hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541594/
https://www.ncbi.nlm.nih.gov/pubmed/37786714
http://dx.doi.org/10.1101/2023.08.25.554815
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