Model-directed generation of CRISPR-Cas13a guide RNAs designs artificial sequences that improve nucleic acid detection

Generating maximally-fit biological sequences has the potential to transform CRISPR guide RNA design as it has other areas of biomedicine. Here, we introduce model-directed exploration algorithms (MEAs) for designing maximally-fit, artificial CRISPR-Cas13a guides—with multiple mismatches to any natu...

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Detalles Bibliográficos
Autores principales: Mantena, Sreekar, Pillai, Priya P., Petros, Brittany A., Welch, Nicole L., Myhrvold, Cameron, Sabeti, Pardis C., Metsky, Hayden C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541601/
https://www.ncbi.nlm.nih.gov/pubmed/37786711
http://dx.doi.org/10.1101/2023.09.20.557569

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