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Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats
INTRODUCTION: Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats. MATERIAL AND METHODS: Twenty-eight adult male albino Wistar rats were assigned to four groups: a nor...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541664/ https://www.ncbi.nlm.nih.gov/pubmed/37786839 http://dx.doi.org/10.2478/jvetres-2023-0039 |
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author | Morgan, Ashraf M. Ogaly, Hanan A. Kamel, Shaimaa Rashad, Maha M. Hassanen, Eman I. Ibrahim, Marwa A. Galal, Mona K. Yassin, Aya M. Dulmani, Sharah A. Al Al-Zahrani, Fatimah A.M. Hussien, Ahmed M. |
author_facet | Morgan, Ashraf M. Ogaly, Hanan A. Kamel, Shaimaa Rashad, Maha M. Hassanen, Eman I. Ibrahim, Marwa A. Galal, Mona K. Yassin, Aya M. Dulmani, Sharah A. Al Al-Zahrani, Fatimah A.M. Hussien, Ahmed M. |
author_sort | Morgan, Ashraf M. |
collection | PubMed |
description | INTRODUCTION: Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats. MATERIAL AND METHODS: Twenty-eight adult male albino Wistar rats were assigned to four groups: a normal control (NC), a PEN group, a NAC group and a PEN+NAC group. Administration of PEN (50 mg/kg body weight (b.w.) every 2 days) and NAC (150 mg/kg b.w., daily) took place via oral gavage for 10 days. RESULTS: Effective amelioration by NAC of PEN-induced liver and kidney dysfunction was indicated by a significant reduction in the circulating liver and kidney markers (aspartate aminotransferase, alanine aminotransferase, urea and creatinine). Attenuation of PEN-induced oxidative stress and lipid peroxidation in liver and kidney tissues was evident in a significant reduction in malondialdehyde and enhanced total antioxidant capacity. Moreover, NAC significantly reduced the histopathological alterations and the expression of tumour necrosis factor α in liver and kidney tissue. Furthermore, NAC maintained the messenger RNA levels of nuclear factor erythroid 2-related factor 2 (Nrf2), haem oxygenase 1, and Kelch-like erythroid cell-derived protein 1 and prevented nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) protein upregulation caused by PEN. CONCLUSION: N-acetyl-1-cysteine protected against PEN-induced hepatorenal oxidative damage and inflammatory response via activation of Nrf2 and inhibition of NF-κB pathways. |
format | Online Article Text |
id | pubmed-10541664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-105416642023-10-02 Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats Morgan, Ashraf M. Ogaly, Hanan A. Kamel, Shaimaa Rashad, Maha M. Hassanen, Eman I. Ibrahim, Marwa A. Galal, Mona K. Yassin, Aya M. Dulmani, Sharah A. Al Al-Zahrani, Fatimah A.M. Hussien, Ahmed M. J Vet Res Article INTRODUCTION: Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats. MATERIAL AND METHODS: Twenty-eight adult male albino Wistar rats were assigned to four groups: a normal control (NC), a PEN group, a NAC group and a PEN+NAC group. Administration of PEN (50 mg/kg body weight (b.w.) every 2 days) and NAC (150 mg/kg b.w., daily) took place via oral gavage for 10 days. RESULTS: Effective amelioration by NAC of PEN-induced liver and kidney dysfunction was indicated by a significant reduction in the circulating liver and kidney markers (aspartate aminotransferase, alanine aminotransferase, urea and creatinine). Attenuation of PEN-induced oxidative stress and lipid peroxidation in liver and kidney tissues was evident in a significant reduction in malondialdehyde and enhanced total antioxidant capacity. Moreover, NAC significantly reduced the histopathological alterations and the expression of tumour necrosis factor α in liver and kidney tissue. Furthermore, NAC maintained the messenger RNA levels of nuclear factor erythroid 2-related factor 2 (Nrf2), haem oxygenase 1, and Kelch-like erythroid cell-derived protein 1 and prevented nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) protein upregulation caused by PEN. CONCLUSION: N-acetyl-1-cysteine protected against PEN-induced hepatorenal oxidative damage and inflammatory response via activation of Nrf2 and inhibition of NF-κB pathways. Sciendo 2023-09-20 /pmc/articles/PMC10541664/ /pubmed/37786839 http://dx.doi.org/10.2478/jvetres-2023-0039 Text en © 2023 Ashraf M. Morgan et al., published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Article Morgan, Ashraf M. Ogaly, Hanan A. Kamel, Shaimaa Rashad, Maha M. Hassanen, Eman I. Ibrahim, Marwa A. Galal, Mona K. Yassin, Aya M. Dulmani, Sharah A. Al Al-Zahrani, Fatimah A.M. Hussien, Ahmed M. Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
title | Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
title_full | Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
title_fullStr | Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
title_full_unstemmed | Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
title_short | Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
title_sort | protective effects of n-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541664/ https://www.ncbi.nlm.nih.gov/pubmed/37786839 http://dx.doi.org/10.2478/jvetres-2023-0039 |
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