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Distribution of carp edema virus in organs of infected juvenile common carp

INTRODUCTION: The disease caused by carp edema virus (CEV) manifests with lethargy as a primary sign; this observation in koi in Japan gained the disease the name koi sleepy disease (KSD). In the years following the discovery of the virus in Japan, KSD cases have been noted in the UK in koi and comm...

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Autores principales: Matras, Marek, Stachnik, Magdalena, Borzym, Ewa, Maj-Paluch, Joanna, Reichert, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541666/
https://www.ncbi.nlm.nih.gov/pubmed/37786850
http://dx.doi.org/10.2478/jvetres-2023-0049
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author Matras, Marek
Stachnik, Magdalena
Borzym, Ewa
Maj-Paluch, Joanna
Reichert, Michał
author_facet Matras, Marek
Stachnik, Magdalena
Borzym, Ewa
Maj-Paluch, Joanna
Reichert, Michał
author_sort Matras, Marek
collection PubMed
description INTRODUCTION: The disease caused by carp edema virus (CEV) manifests with lethargy as a primary sign; this observation in koi in Japan gained the disease the name koi sleepy disease (KSD). In the years following the discovery of the virus in Japan, KSD cases have been noted in the UK in koi and common carp. Conducting research in order to expand knowledge of the processes of distribution of CEV in infected fish organs will be helpful for eradication and diagnostic purposes. MATERIAL AND METHODS: Carp edema virus–affected fish with clinical signs of KSD were experimentally cohabited with common carp fry (30 fish). Three fish were euthanised by bath in a 0.5 g L(−1) tricaine solution at one week intervals (7, 14, 21 and 28 days post cohabitation). Tissue samples from the brain, gills, spleen, kidney, intestines and skin were collected, and the total DNA was extracted and tested by real-time PCR. RESULTS: By the seventh day post infection, CEV DNA was most often found in the skin, gills and brain and less frequently in the kidney and intestines. In many of the common carp fry, CEV DNA could typically be found in several organs of each individual fish, although it was only found in one sample of spleen tissue. CONCLUSION: In this experimental study the pathogenesis of the CEV infection process was shown, the high infectivity of CEV was confirmed and the best organs were determined for sampling in CEV-infection experimentation. The real-time PCR method used in our cohabitation experiments was shown to be useful at the clinical and asymptomatic stage of virus infection.
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spelling pubmed-105416662023-10-02 Distribution of carp edema virus in organs of infected juvenile common carp Matras, Marek Stachnik, Magdalena Borzym, Ewa Maj-Paluch, Joanna Reichert, Michał J Vet Res Article INTRODUCTION: The disease caused by carp edema virus (CEV) manifests with lethargy as a primary sign; this observation in koi in Japan gained the disease the name koi sleepy disease (KSD). In the years following the discovery of the virus in Japan, KSD cases have been noted in the UK in koi and common carp. Conducting research in order to expand knowledge of the processes of distribution of CEV in infected fish organs will be helpful for eradication and diagnostic purposes. MATERIAL AND METHODS: Carp edema virus–affected fish with clinical signs of KSD were experimentally cohabited with common carp fry (30 fish). Three fish were euthanised by bath in a 0.5 g L(−1) tricaine solution at one week intervals (7, 14, 21 and 28 days post cohabitation). Tissue samples from the brain, gills, spleen, kidney, intestines and skin were collected, and the total DNA was extracted and tested by real-time PCR. RESULTS: By the seventh day post infection, CEV DNA was most often found in the skin, gills and brain and less frequently in the kidney and intestines. In many of the common carp fry, CEV DNA could typically be found in several organs of each individual fish, although it was only found in one sample of spleen tissue. CONCLUSION: In this experimental study the pathogenesis of the CEV infection process was shown, the high infectivity of CEV was confirmed and the best organs were determined for sampling in CEV-infection experimentation. The real-time PCR method used in our cohabitation experiments was shown to be useful at the clinical and asymptomatic stage of virus infection. Sciendo 2023-09-20 /pmc/articles/PMC10541666/ /pubmed/37786850 http://dx.doi.org/10.2478/jvetres-2023-0049 Text en © 2023 Marek Matras et al., published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
spellingShingle Article
Matras, Marek
Stachnik, Magdalena
Borzym, Ewa
Maj-Paluch, Joanna
Reichert, Michał
Distribution of carp edema virus in organs of infected juvenile common carp
title Distribution of carp edema virus in organs of infected juvenile common carp
title_full Distribution of carp edema virus in organs of infected juvenile common carp
title_fullStr Distribution of carp edema virus in organs of infected juvenile common carp
title_full_unstemmed Distribution of carp edema virus in organs of infected juvenile common carp
title_short Distribution of carp edema virus in organs of infected juvenile common carp
title_sort distribution of carp edema virus in organs of infected juvenile common carp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541666/
https://www.ncbi.nlm.nih.gov/pubmed/37786850
http://dx.doi.org/10.2478/jvetres-2023-0049
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