Cargando…
Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro
Influenza H7N9 virus causes human infections with about 40% case fatality rate. The severe cases usually present with pneumonia; however, some present with central nervous system complications. Pneumonia syndrome is attributed to the cytokine storm after infection with H7N9, but the pathogenic mecha...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541704/ https://www.ncbi.nlm.nih.gov/pubmed/37773164 http://dx.doi.org/10.1186/s12985-023-02163-3 |
_version_ | 1785113953986150400 |
---|---|
author | Lei, Yuxuan Sun, Ying Wu, Weihua Liu, Hui Wang, Xin Shu, Yuelong Fang, Shisong |
author_facet | Lei, Yuxuan Sun, Ying Wu, Weihua Liu, Hui Wang, Xin Shu, Yuelong Fang, Shisong |
author_sort | Lei, Yuxuan |
collection | PubMed |
description | Influenza H7N9 virus causes human infections with about 40% case fatality rate. The severe cases usually present with pneumonia; however, some present with central nervous system complications. Pneumonia syndrome is attributed to the cytokine storm after infection with H7N9, but the pathogenic mechanism of central nervous system complications has not been clarified. This study used immortalized human brain microvascular endothelial cells hCMEC/D3 to simulate the blood-brain barrier. It demonstrated that H7N9 virus could infect brain microvascular endothelial cells and compromise the blood-brain barrier integrity and permeability by down-regulating the expression of cell junction-related proteins, including claudin-5, occludin, and vascular endothelial (VE)-cadherin. These results suggested that H7N9 could infect the blood-brain barrier in vitro and affect its functions, which could be a potential mechanism for the pathogenesis of H7N9 viral encephalopathy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02163-3. |
format | Online Article Text |
id | pubmed-10541704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105417042023-10-02 Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro Lei, Yuxuan Sun, Ying Wu, Weihua Liu, Hui Wang, Xin Shu, Yuelong Fang, Shisong Virol J Research Influenza H7N9 virus causes human infections with about 40% case fatality rate. The severe cases usually present with pneumonia; however, some present with central nervous system complications. Pneumonia syndrome is attributed to the cytokine storm after infection with H7N9, but the pathogenic mechanism of central nervous system complications has not been clarified. This study used immortalized human brain microvascular endothelial cells hCMEC/D3 to simulate the blood-brain barrier. It demonstrated that H7N9 virus could infect brain microvascular endothelial cells and compromise the blood-brain barrier integrity and permeability by down-regulating the expression of cell junction-related proteins, including claudin-5, occludin, and vascular endothelial (VE)-cadherin. These results suggested that H7N9 could infect the blood-brain barrier in vitro and affect its functions, which could be a potential mechanism for the pathogenesis of H7N9 viral encephalopathy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02163-3. BioMed Central 2023-09-29 /pmc/articles/PMC10541704/ /pubmed/37773164 http://dx.doi.org/10.1186/s12985-023-02163-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Lei, Yuxuan Sun, Ying Wu, Weihua Liu, Hui Wang, Xin Shu, Yuelong Fang, Shisong Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
title | Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
title_full | Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
title_fullStr | Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
title_full_unstemmed | Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
title_short | Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
title_sort | influenza h7n9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541704/ https://www.ncbi.nlm.nih.gov/pubmed/37773164 http://dx.doi.org/10.1186/s12985-023-02163-3 |
work_keys_str_mv | AT leiyuxuan influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro AT sunying influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro AT wuweihua influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro AT liuhui influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro AT wangxin influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro AT shuyuelong influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro AT fangshisong influenzah7n9virusdisruptsthemonolayerhumanbrainmicrovascularendothelialcellsbarrierinvitro |