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Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets
The objective of the study was to assess the comparative effects of Vernonia amygdalina leaf meal (VALM) and zinc (Zn) on the serum proinflammatory and anti-inflammatory cytokines as well as DNA damage of cocks-fed aflatoxin B(1) (AFB(1)) contaminated diets. A total of 250 sexually mature Isa White...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541856/ https://www.ncbi.nlm.nih.gov/pubmed/37786424 http://dx.doi.org/10.1093/tas/txad113 |
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author | Olarotimi, Olumuyiwa J Gbore, Francis A Oloruntola, Olugbenga D Jimoh, Olatunji A |
author_facet | Olarotimi, Olumuyiwa J Gbore, Francis A Oloruntola, Olugbenga D Jimoh, Olatunji A |
author_sort | Olarotimi, Olumuyiwa J |
collection | PubMed |
description | The objective of the study was to assess the comparative effects of Vernonia amygdalina leaf meal (VALM) and zinc (Zn) on the serum proinflammatory and anti-inflammatory cytokines as well as DNA damage of cocks-fed aflatoxin B(1) (AFB(1)) contaminated diets. A total of 250 sexually mature Isa White cocks of 24 weeks old were randomly distributed into five groups (treatments) with each containing 50 birds, which was replicated five times with 10 birds per replicate. Cocks in group A were fed basal diet only, group B was fed basal diet contaminated with 1 mg AFB(1)/kg diet, group C received diet B (basal + 1 mg/kg AFB(1)) with 50 mg/kg Zn, group D was fed diet B with 2.5 g/kg VALM, and group E received diet B with 5.0 g/kg VALM, respectively. Feed and water were supplied ad libitum with fresh feed added to the feed troughs at 6:00 a.m. and 6:00 p.m., respectively. While serum tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), 8-hydroxy-2ʹ-deoxyguanosine (8-OHdG), and nuclear factor kappa B (NF-κB) were significantly (P < 0.05) elevated among the cocks on diet B, significant (P < 0.05) reductions were recorded among cocks on diets C, D, and E. Conversely, birds in group B had significant (P < 0.05) depression in serum interleukin 4 (IL-4) and interleukin 10 (IL-10) while improvements (P < 0.05) were recorded among cocks in groups C, D, and E, respectively. Therefore, the inclusion of VALM offset the adverse physiological effects of AFB(1) observed among group B birds. The effects were comparable with the results presented by the cocksfed diet containing Zn. |
format | Online Article Text |
id | pubmed-10541856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105418562023-10-02 Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets Olarotimi, Olumuyiwa J Gbore, Francis A Oloruntola, Olugbenga D Jimoh, Olatunji A Transl Anim Sci Animal Health and Well Being The objective of the study was to assess the comparative effects of Vernonia amygdalina leaf meal (VALM) and zinc (Zn) on the serum proinflammatory and anti-inflammatory cytokines as well as DNA damage of cocks-fed aflatoxin B(1) (AFB(1)) contaminated diets. A total of 250 sexually mature Isa White cocks of 24 weeks old were randomly distributed into five groups (treatments) with each containing 50 birds, which was replicated five times with 10 birds per replicate. Cocks in group A were fed basal diet only, group B was fed basal diet contaminated with 1 mg AFB(1)/kg diet, group C received diet B (basal + 1 mg/kg AFB(1)) with 50 mg/kg Zn, group D was fed diet B with 2.5 g/kg VALM, and group E received diet B with 5.0 g/kg VALM, respectively. Feed and water were supplied ad libitum with fresh feed added to the feed troughs at 6:00 a.m. and 6:00 p.m., respectively. While serum tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), 8-hydroxy-2ʹ-deoxyguanosine (8-OHdG), and nuclear factor kappa B (NF-κB) were significantly (P < 0.05) elevated among the cocks on diet B, significant (P < 0.05) reductions were recorded among cocks on diets C, D, and E. Conversely, birds in group B had significant (P < 0.05) depression in serum interleukin 4 (IL-4) and interleukin 10 (IL-10) while improvements (P < 0.05) were recorded among cocks in groups C, D, and E, respectively. Therefore, the inclusion of VALM offset the adverse physiological effects of AFB(1) observed among group B birds. The effects were comparable with the results presented by the cocksfed diet containing Zn. Oxford University Press 2023-09-16 /pmc/articles/PMC10541856/ /pubmed/37786424 http://dx.doi.org/10.1093/tas/txad113 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Health and Well Being Olarotimi, Olumuyiwa J Gbore, Francis A Oloruntola, Olugbenga D Jimoh, Olatunji A Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets |
title | Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets |
title_full | Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets |
title_fullStr | Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets |
title_full_unstemmed | Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets |
title_short | Serum inflammation and oxidative DNA damage amelioration in cocks-fed supplemental Vernonia amygdalina and zinc in aflatoxin B(1) contaminated diets |
title_sort | serum inflammation and oxidative dna damage amelioration in cocks-fed supplemental vernonia amygdalina and zinc in aflatoxin b(1) contaminated diets |
topic | Animal Health and Well Being |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541856/ https://www.ncbi.nlm.nih.gov/pubmed/37786424 http://dx.doi.org/10.1093/tas/txad113 |
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