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Demonstration of in vivo engineered tandem duplications of varying sizes using CRISPR and recombinases in Drosophila melanogaster

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Tandem gene duplicates are important parts of eukaryotic genome structure, yet the phenotypic effects of new tandem duplications are not well-understood, in part owing to a lack of techniques to build and modify them. We introduce a method, Recombinase-Mediated Tandem Duplication, to engineer specif...

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Detalles Bibliográficos
Autores principales:	Loehlin, David W, McClain, Georgia L, Xu, Manting, Kedia, Ria, Root, Elise
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Investigation
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542505/ https://www.ncbi.nlm.nih.gov/pubmed/37462278 http://dx.doi.org/10.1093/g3journal/jkad155

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