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A dual-binding magnetic immunoassay to predict spontaneous preterm birth

Complications posed by preterm birth (delivery before 37 weeks of pregnancy) are a leading cause of newborn morbidity and mortality. The previous discovery and validation of an algorithm that includes maternal serum protein biomarkers, sex hormone-binding globulin (SHBG), and insulin-like growth fac...

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Autores principales: Sveiven, Michael, Gassman, Andrew, Rosenberg, Joshua, Chan, Matthew, Boniface, Jay, O’Donoghue, Anthony J., Laurent, Louise C., Hall, Drew A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542577/
https://www.ncbi.nlm.nih.gov/pubmed/37790251
http://dx.doi.org/10.3389/fbioe.2023.1256267
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author Sveiven, Michael
Gassman, Andrew
Rosenberg, Joshua
Chan, Matthew
Boniface, Jay
O’Donoghue, Anthony J.
Laurent, Louise C.
Hall, Drew A.
author_facet Sveiven, Michael
Gassman, Andrew
Rosenberg, Joshua
Chan, Matthew
Boniface, Jay
O’Donoghue, Anthony J.
Laurent, Louise C.
Hall, Drew A.
author_sort Sveiven, Michael
collection PubMed
description Complications posed by preterm birth (delivery before 37 weeks of pregnancy) are a leading cause of newborn morbidity and mortality. The previous discovery and validation of an algorithm that includes maternal serum protein biomarkers, sex hormone-binding globulin (SHBG), and insulin-like growth factor-binding protein 4 (IBP4), with clinical factors to predict preterm birth represents an opportunity for the development of a widely accessible point-of-care assay to guide clinical management. Toward this end, we developed SHBG and IBP4 quantification assays for maternal serum using giant magnetoresistive (GMR) sensors and a self-normalizing dual-binding magnetic immunoassay. The assays have a picomolar limit of detections (LOD) with a relatively broad dynamic range that covers the physiological level of the analytes as they change throughout gestation. Measurement of serum from pregnant donors using the GMR assays was highly concordant with those obtained using a clinical mass spectrometry (MS)-based assay for the same protein markers. The MS assay requires capitally intense equipment and highly trained operators with a few days turnaround time, whereas the GMR assays can be performed in minutes on small, inexpensive instruments with minimal personnel training and microfluidic automation. The potential for high sensitivity, accuracy, and speed of the GMR assays, along with low equipment and personnel requirements, make them good candidates for developing point-of-care tests. Rapid turnaround risk assessment for preterm birth would enable patient testing and counseling at the same clinic visit, thereby increasing the timeliness of recommended interventions.
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spelling pubmed-105425772023-10-03 A dual-binding magnetic immunoassay to predict spontaneous preterm birth Sveiven, Michael Gassman, Andrew Rosenberg, Joshua Chan, Matthew Boniface, Jay O’Donoghue, Anthony J. Laurent, Louise C. Hall, Drew A. Front Bioeng Biotechnol Bioengineering and Biotechnology Complications posed by preterm birth (delivery before 37 weeks of pregnancy) are a leading cause of newborn morbidity and mortality. The previous discovery and validation of an algorithm that includes maternal serum protein biomarkers, sex hormone-binding globulin (SHBG), and insulin-like growth factor-binding protein 4 (IBP4), with clinical factors to predict preterm birth represents an opportunity for the development of a widely accessible point-of-care assay to guide clinical management. Toward this end, we developed SHBG and IBP4 quantification assays for maternal serum using giant magnetoresistive (GMR) sensors and a self-normalizing dual-binding magnetic immunoassay. The assays have a picomolar limit of detections (LOD) with a relatively broad dynamic range that covers the physiological level of the analytes as they change throughout gestation. Measurement of serum from pregnant donors using the GMR assays was highly concordant with those obtained using a clinical mass spectrometry (MS)-based assay for the same protein markers. The MS assay requires capitally intense equipment and highly trained operators with a few days turnaround time, whereas the GMR assays can be performed in minutes on small, inexpensive instruments with minimal personnel training and microfluidic automation. The potential for high sensitivity, accuracy, and speed of the GMR assays, along with low equipment and personnel requirements, make them good candidates for developing point-of-care tests. Rapid turnaround risk assessment for preterm birth would enable patient testing and counseling at the same clinic visit, thereby increasing the timeliness of recommended interventions. Frontiers Media S.A. 2023-09-13 /pmc/articles/PMC10542577/ /pubmed/37790251 http://dx.doi.org/10.3389/fbioe.2023.1256267 Text en Copyright © 2023 Sveiven, Gassman, Rosenberg, Chan, Boniface, O’Donoghue, Laurent and Hall. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Sveiven, Michael
Gassman, Andrew
Rosenberg, Joshua
Chan, Matthew
Boniface, Jay
O’Donoghue, Anthony J.
Laurent, Louise C.
Hall, Drew A.
A dual-binding magnetic immunoassay to predict spontaneous preterm birth
title A dual-binding magnetic immunoassay to predict spontaneous preterm birth
title_full A dual-binding magnetic immunoassay to predict spontaneous preterm birth
title_fullStr A dual-binding magnetic immunoassay to predict spontaneous preterm birth
title_full_unstemmed A dual-binding magnetic immunoassay to predict spontaneous preterm birth
title_short A dual-binding magnetic immunoassay to predict spontaneous preterm birth
title_sort dual-binding magnetic immunoassay to predict spontaneous preterm birth
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542577/
https://www.ncbi.nlm.nih.gov/pubmed/37790251
http://dx.doi.org/10.3389/fbioe.2023.1256267
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