Cargando…

Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis

INTRODUCTION: There is an urgent need to develop therapeutic options for biofilm-producing Staphylococcus aureus (S. aureus). Therefore, the renewed interest in essential oils (EOs), especially carvacrol, linalool and eugenol, has attracted the attention of our research group. METHODS: Multidrug res...

Descripción completa

Detalles Bibliográficos
Autores principales: Abd El-Hamid, Marwa I., El-Tarabili, Reham M., Bahnass, Mosa M., Alshahrani, Mohammed Abdulrahman, Saif, Ahmed, Alwutayd, Khairiah Mubarak, Safhi, Fatmah Ahmed, Mansour, Abdallah Tageldein, Alblwi, Noaf Abdullah N., Ghoneim, Mohammed M., Elmaaty, Ayman Abo, Al-harthi, Helal F., Bendary, Mahmoud M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542579/
https://www.ncbi.nlm.nih.gov/pubmed/37790910
http://dx.doi.org/10.3389/fcimb.2023.1265027
_version_ 1785114121051570176
author Abd El-Hamid, Marwa I.
El-Tarabili, Reham M.
Bahnass, Mosa M.
Alshahrani, Mohammed Abdulrahman
Saif, Ahmed
Alwutayd, Khairiah Mubarak
Safhi, Fatmah Ahmed
Mansour, Abdallah Tageldein
Alblwi, Noaf Abdullah N.
Ghoneim, Mohammed M.
Elmaaty, Ayman Abo
Al-harthi, Helal F.
Bendary, Mahmoud M.
author_facet Abd El-Hamid, Marwa I.
El-Tarabili, Reham M.
Bahnass, Mosa M.
Alshahrani, Mohammed Abdulrahman
Saif, Ahmed
Alwutayd, Khairiah Mubarak
Safhi, Fatmah Ahmed
Mansour, Abdallah Tageldein
Alblwi, Noaf Abdullah N.
Ghoneim, Mohammed M.
Elmaaty, Ayman Abo
Al-harthi, Helal F.
Bendary, Mahmoud M.
author_sort Abd El-Hamid, Marwa I.
collection PubMed
description INTRODUCTION: There is an urgent need to develop therapeutic options for biofilm-producing Staphylococcus aureus (S. aureus). Therefore, the renewed interest in essential oils (EOs), especially carvacrol, linalool and eugenol, has attracted the attention of our research group. METHODS: Multidrug resistance and multivirulence profiles in addition to biofilm production of S. aureus strains isolated from cows with mastitis were evaluated using both phenotypic and genotypic methods. The antimicrobial and antibiofilm activities of EOs were tested using both in vitro and molecular docking studies. Moreover, the interactions between commonly used antibiotics and the tested EOs were detected using the checkerboard method. RESULTS: We found that all our isolates (n= 37) were biofilm methicillin resistant S. aureus (MRSA) producers and 40.5% were vancomycin resistant S. aureus (VRSA). Unfortunately, 73 and 43.2% of the recovered MRSA isolates showed multidrug resistant (MDR) and multivirulence patterns, respectively. The antimicrobial activities of the tested EOs matched with the phenotypic evaluation of the antibiofilm activities and molecular docking studies. Linalool showed the highest antimicrobial and antibiofilm activities, followed by carvacrol and eugenol EOs. Fortunately, synergistic interactions between the investigated EOs and methicillin or vancomycin were detected with fractional inhibitory concentration index (FICI) values ≤ 0.5. Moreover, the antimicrobial resistance patterns of 13 isolates changed to sensitive phenotypes after treatment with any of the investigated EOs. Treatment failure of bovine mastitis with resistant S. aureus can be avoided by combining the investigated EOs with available antimicrobial drugs. CONCLUSION: We hope that our findings can be translated into a formulation of new pharmaceutical dosage forms against biofilm-producing S. aureus pathogens.
format Online
Article
Text
id pubmed-10542579
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105425792023-10-03 Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis Abd El-Hamid, Marwa I. El-Tarabili, Reham M. Bahnass, Mosa M. Alshahrani, Mohammed Abdulrahman Saif, Ahmed Alwutayd, Khairiah Mubarak Safhi, Fatmah Ahmed Mansour, Abdallah Tageldein Alblwi, Noaf Abdullah N. Ghoneim, Mohammed M. Elmaaty, Ayman Abo Al-harthi, Helal F. Bendary, Mahmoud M. Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: There is an urgent need to develop therapeutic options for biofilm-producing Staphylococcus aureus (S. aureus). Therefore, the renewed interest in essential oils (EOs), especially carvacrol, linalool and eugenol, has attracted the attention of our research group. METHODS: Multidrug resistance and multivirulence profiles in addition to biofilm production of S. aureus strains isolated from cows with mastitis were evaluated using both phenotypic and genotypic methods. The antimicrobial and antibiofilm activities of EOs were tested using both in vitro and molecular docking studies. Moreover, the interactions between commonly used antibiotics and the tested EOs were detected using the checkerboard method. RESULTS: We found that all our isolates (n= 37) were biofilm methicillin resistant S. aureus (MRSA) producers and 40.5% were vancomycin resistant S. aureus (VRSA). Unfortunately, 73 and 43.2% of the recovered MRSA isolates showed multidrug resistant (MDR) and multivirulence patterns, respectively. The antimicrobial activities of the tested EOs matched with the phenotypic evaluation of the antibiofilm activities and molecular docking studies. Linalool showed the highest antimicrobial and antibiofilm activities, followed by carvacrol and eugenol EOs. Fortunately, synergistic interactions between the investigated EOs and methicillin or vancomycin were detected with fractional inhibitory concentration index (FICI) values ≤ 0.5. Moreover, the antimicrobial resistance patterns of 13 isolates changed to sensitive phenotypes after treatment with any of the investigated EOs. Treatment failure of bovine mastitis with resistant S. aureus can be avoided by combining the investigated EOs with available antimicrobial drugs. CONCLUSION: We hope that our findings can be translated into a formulation of new pharmaceutical dosage forms against biofilm-producing S. aureus pathogens. Frontiers Media S.A. 2023-09-15 /pmc/articles/PMC10542579/ /pubmed/37790910 http://dx.doi.org/10.3389/fcimb.2023.1265027 Text en Copyright © 2023 Abd El-Hamid, El-Tarabili, Bahnass, Alshahrani, Saif, Alwutayd, Safhi, Mansour, Alblwi, Ghoneim, Elmaaty, Al-harthi and Bendary https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Abd El-Hamid, Marwa I.
El-Tarabili, Reham M.
Bahnass, Mosa M.
Alshahrani, Mohammed Abdulrahman
Saif, Ahmed
Alwutayd, Khairiah Mubarak
Safhi, Fatmah Ahmed
Mansour, Abdallah Tageldein
Alblwi, Noaf Abdullah N.
Ghoneim, Mohammed M.
Elmaaty, Ayman Abo
Al-harthi, Helal F.
Bendary, Mahmoud M.
Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis
title Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis
title_full Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis
title_fullStr Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis
title_full_unstemmed Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis
title_short Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis
title_sort partnering essential oils with antibiotics: proven therapies against bovine staphylococcus aureus mastitis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542579/
https://www.ncbi.nlm.nih.gov/pubmed/37790910
http://dx.doi.org/10.3389/fcimb.2023.1265027
work_keys_str_mv AT abdelhamidmarwai partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT eltarabilirehamm partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT bahnassmosam partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT alshahranimohammedabdulrahman partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT saifahmed partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT alwutaydkhairiahmubarak partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT safhifatmahahmed partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT mansourabdallahtageldein partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT alblwinoafabdullahn partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT ghoneimmohammedm partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT elmaatyaymanabo partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT alharthihelalf partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis
AT bendarymahmoudm partneringessentialoilswithantibioticsproventherapiesagainstbovinestaphylococcusaureusmastitis