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Using PrISMa to reveal the interactome of the human claudins family

Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass s...

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Detalles Bibliográficos
Autores principales: Perez-Hernandez, Daniel, Suarez-Artiles, Lorena, Jones, Mattson S.O., Dittmar, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542633/
https://www.ncbi.nlm.nih.gov/pubmed/37756153
http://dx.doi.org/10.1016/j.xpro.2023.102549