Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA

Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific ge...

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Detalles Bibliográficos
Autores principales: Matsui, Hidenori, Suzuki, Masato, Aoki, Sae, Shibayama, Keigo, Tokunaga, Kengo, Suzuki, Hidekazu, Mabe, Katsuhiro, Kenri, Tsuyoshi, Rimbara, Emiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542635/
https://www.ncbi.nlm.nih.gov/pubmed/37756155
http://dx.doi.org/10.1016/j.xpro.2023.102556
Descripción
Sumario:Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific gene. We then define procedures for inoculating biopsies onto primary agar plates and transferring colonies to secondary agar plates. Finally, we detail whole-genome sequencing of bacteria and assess H. suis infection in sera with ELISA. For complete details on the use and execution of these protocols, please refer to Matsui et al.(1)

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