Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA

Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific ge...

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Autores principales: Matsui, Hidenori, Suzuki, Masato, Aoki, Sae, Shibayama, Keigo, Tokunaga, Kengo, Suzuki, Hidekazu, Mabe, Katsuhiro, Kenri, Tsuyoshi, Rimbara, Emiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542635/
https://www.ncbi.nlm.nih.gov/pubmed/37756155
http://dx.doi.org/10.1016/j.xpro.2023.102556
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author Matsui, Hidenori
Suzuki, Masato
Aoki, Sae
Shibayama, Keigo
Tokunaga, Kengo
Suzuki, Hidekazu
Mabe, Katsuhiro
Kenri, Tsuyoshi
Rimbara, Emiko
author_facet Matsui, Hidenori
Suzuki, Masato
Aoki, Sae
Shibayama, Keigo
Tokunaga, Kengo
Suzuki, Hidekazu
Mabe, Katsuhiro
Kenri, Tsuyoshi
Rimbara, Emiko
author_sort Matsui, Hidenori
collection PubMed
description Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific gene. We then define procedures for inoculating biopsies onto primary agar plates and transferring colonies to secondary agar plates. Finally, we detail whole-genome sequencing of bacteria and assess H. suis infection in sera with ELISA. For complete details on the use and execution of these protocols, please refer to Matsui et al.(1)
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spelling pubmed-105426352023-10-03 Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA Matsui, Hidenori Suzuki, Masato Aoki, Sae Shibayama, Keigo Tokunaga, Kengo Suzuki, Hidekazu Mabe, Katsuhiro Kenri, Tsuyoshi Rimbara, Emiko STAR Protoc Protocol Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific gene. We then define procedures for inoculating biopsies onto primary agar plates and transferring colonies to secondary agar plates. Finally, we detail whole-genome sequencing of bacteria and assess H. suis infection in sera with ELISA. For complete details on the use and execution of these protocols, please refer to Matsui et al.(1) Elsevier 2023-09-26 /pmc/articles/PMC10542635/ /pubmed/37756155 http://dx.doi.org/10.1016/j.xpro.2023.102556 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Matsui, Hidenori
Suzuki, Masato
Aoki, Sae
Shibayama, Keigo
Tokunaga, Kengo
Suzuki, Hidekazu
Mabe, Katsuhiro
Kenri, Tsuyoshi
Rimbara, Emiko
Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
title Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
title_full Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
title_fullStr Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
title_full_unstemmed Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
title_short Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
title_sort protocol for detecting helicobacter suis infection in gastric biopsies and serum by pcr and elisa
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542635/
https://www.ncbi.nlm.nih.gov/pubmed/37756155
http://dx.doi.org/10.1016/j.xpro.2023.102556
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