Detection of P. malariae using a new rapid isothermal amplification lateral flow assay

P. malariae is found worldwide and causes chronic parasitism in its human hosts. We developed a P. malariae (Pm) diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection. Using 18S rRNA plasmid DNA, the assay demonstrates a detection li...

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Detalles Bibliográficos
Autores principales: Assefa, Ashenafi, Wamae, Kevin K., Hennelly, Chris M., Ngasala, Billy, Muller, Meredith, Kalonji, Albert, Phanzu, Fernandine, Cunningham, Clark H., Lin, Jessica T., Parr, Jonathan B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543045/
https://www.ncbi.nlm.nih.gov/pubmed/37790493
http://dx.doi.org/10.1101/2023.02.26.23286371
Descripción
Sumario:P. malariae is found worldwide and causes chronic parasitism in its human hosts. We developed a P. malariae (Pm) diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection. Using 18S rRNA plasmid DNA, the assay demonstrates a detection limit of 10 copies /μL (~1.7 genome equivalents) and 100% analytical specificity. Testing in field samples showed 95% clinical sensitivity and 88% specificity compared to qPCR. Total assay time was 35 minutes. Combined with simplified DNA extraction methods, the assay has potential for future field-deployable point-of-care use to detect a parasite species that remains largely undiagnosed.

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