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Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform

Developing effective strategies for the flexible control of fluid is vital for microfluidic electrochemical biosensing. In this study, a gigahertz (GHz) acoustic streaming (AS) based sonoelectrochemical system was developed to realize an on-chip surface modification and sensitive hydrogen peroxide (...

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Autores principales: Zhu, Feng, Liu, Zeyu, Wu, Xiaoyu, Xu, Die, Li, Quanning, Chen, Xuejiao, Pang, Wei, Duan, Xuexin, Wang, Yanyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543187/
https://www.ncbi.nlm.nih.gov/pubmed/37769590
http://dx.doi.org/10.1016/j.ultsonch.2023.106618
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author Zhu, Feng
Liu, Zeyu
Wu, Xiaoyu
Xu, Die
Li, Quanning
Chen, Xuejiao
Pang, Wei
Duan, Xuexin
Wang, Yanyan
author_facet Zhu, Feng
Liu, Zeyu
Wu, Xiaoyu
Xu, Die
Li, Quanning
Chen, Xuejiao
Pang, Wei
Duan, Xuexin
Wang, Yanyan
author_sort Zhu, Feng
collection PubMed
description Developing effective strategies for the flexible control of fluid is vital for microfluidic electrochemical biosensing. In this study, a gigahertz (GHz) acoustic streaming (AS) based sonoelectrochemical system was developed to realize an on-chip surface modification and sensitive hydrogen peroxide (H(2)O(2)) detection from living cells. The flexible and controlled fluid surrounding the electrochemical chip was optimized theoretically and applied in the sonoelectrochemical deposition of Au nanoparticles (AuNPs) first. Under the steady and fast flow stimulus of AS, AuNPs could be synthesized with a smaller and evener size distribution than the normal condition, allowing AuNPs to show an excellent peroxidase-like activity. Moreover, the AS also accelerated the mass transport of target molecules and improved the catalytic rate, leading to the enhancement of H(2)O(2) detection, with an extremely low detection limit of 32 nM and a high sensitivity of 4.34 μA/ (mM·mm(2)). Finally, this system was successfully applied in tracking H(2)O(2) release from different cell lines to distinguish the cancer cells from normal cells. This study innovatively integrated the surface modification and molecules detection process on a chip, and also proposed a simple but sensitive platform for microfluidic biosensing application.
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spelling pubmed-105431872023-10-03 Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform Zhu, Feng Liu, Zeyu Wu, Xiaoyu Xu, Die Li, Quanning Chen, Xuejiao Pang, Wei Duan, Xuexin Wang, Yanyan Ultrason Sonochem Original Research Article Developing effective strategies for the flexible control of fluid is vital for microfluidic electrochemical biosensing. In this study, a gigahertz (GHz) acoustic streaming (AS) based sonoelectrochemical system was developed to realize an on-chip surface modification and sensitive hydrogen peroxide (H(2)O(2)) detection from living cells. The flexible and controlled fluid surrounding the electrochemical chip was optimized theoretically and applied in the sonoelectrochemical deposition of Au nanoparticles (AuNPs) first. Under the steady and fast flow stimulus of AS, AuNPs could be synthesized with a smaller and evener size distribution than the normal condition, allowing AuNPs to show an excellent peroxidase-like activity. Moreover, the AS also accelerated the mass transport of target molecules and improved the catalytic rate, leading to the enhancement of H(2)O(2) detection, with an extremely low detection limit of 32 nM and a high sensitivity of 4.34 μA/ (mM·mm(2)). Finally, this system was successfully applied in tracking H(2)O(2) release from different cell lines to distinguish the cancer cells from normal cells. This study innovatively integrated the surface modification and molecules detection process on a chip, and also proposed a simple but sensitive platform for microfluidic biosensing application. Elsevier 2023-09-23 /pmc/articles/PMC10543187/ /pubmed/37769590 http://dx.doi.org/10.1016/j.ultsonch.2023.106618 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Zhu, Feng
Liu, Zeyu
Wu, Xiaoyu
Xu, Die
Li, Quanning
Chen, Xuejiao
Pang, Wei
Duan, Xuexin
Wang, Yanyan
Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
title Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
title_full Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
title_fullStr Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
title_full_unstemmed Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
title_short Enhanced on-Chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
title_sort enhanced on-chip modification and intracellular hydrogen peroxide detection via gigahertz acoustic streaming microfluidic platform
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543187/
https://www.ncbi.nlm.nih.gov/pubmed/37769590
http://dx.doi.org/10.1016/j.ultsonch.2023.106618
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