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Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction

INTRODUCTION: Spodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects. METHODS: Here, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rD...

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Autores principales: Fu, Junrui, Wang, Junhan, Huang, Ximei, Guan, Boyang, Feng, Qili, Deng, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543693/
https://www.ncbi.nlm.nih.gov/pubmed/37789854
http://dx.doi.org/10.3389/fmicb.2023.1237684
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author Fu, Junrui
Wang, Junhan
Huang, Ximei
Guan, Boyang
Feng, Qili
Deng, Huimin
author_facet Fu, Junrui
Wang, Junhan
Huang, Ximei
Guan, Boyang
Feng, Qili
Deng, Huimin
author_sort Fu, Junrui
collection PubMed
description INTRODUCTION: Spodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects. METHODS: Here, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction. RESULTS: Proteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction. DISCUSSION: Our findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota.
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spelling pubmed-105436932023-10-03 Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction Fu, Junrui Wang, Junhan Huang, Ximei Guan, Boyang Feng, Qili Deng, Huimin Front Microbiol Microbiology INTRODUCTION: Spodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects. METHODS: Here, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction. RESULTS: Proteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction. DISCUSSION: Our findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota. Frontiers Media S.A. 2023-09-18 /pmc/articles/PMC10543693/ /pubmed/37789854 http://dx.doi.org/10.3389/fmicb.2023.1237684 Text en Copyright © 2023 Fu, Wang, Huang, Guan, Feng and Deng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fu, Junrui
Wang, Junhan
Huang, Ximei
Guan, Boyang
Feng, Qili
Deng, Huimin
Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
title Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
title_full Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
title_fullStr Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
title_full_unstemmed Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
title_short Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
title_sort composition and diversity of gut microbiota across developmental stages of spodoptera frugiperda and its effect on the reproduction
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543693/
https://www.ncbi.nlm.nih.gov/pubmed/37789854
http://dx.doi.org/10.3389/fmicb.2023.1237684
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